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AB326886

Mouse PAI1 (SERPINE1) ELISA Kit, Chemiluminescent

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Mouse PAI1 (SERPINE1) ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse PAI1 (SERPINE1) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 78.13 - 10000 pg/ml with a sensitivity of 43.37 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

Mr1, Pai1, Planh1, Serpine1, Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1

5 Images
Sandwich ELISA - Mouse PAI1 (SERPINE1) ELISA Kit, Chemiluminescent (AB326886)
  • sELISA

Lab

Sandwich ELISA - Mouse PAI1 (SERPINE1) ELISA Kit, Chemiluminescent (AB326886)

Dynamic range comparison between the Chemiluminescent Mouse PAI1 SimpleStep ELISA, colorimetric Mouse PAI1 SimpleStep ELISA (ab197752), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased dynamic range.

Sandwich ELISA - Mouse PAI1 (SERPINE1) ELISA Kit, Chemiluminescent (AB326886)
  • sELISA

Lab

Sandwich ELISA - Mouse PAI1 (SERPINE1) ELISA Kit, Chemiluminescent (AB326886)

Standard curve comparison between the Chemiluminescent Mouse PAI1 SimpleStep ELISA and colorimetric Mouse PAI1 SimpleStep ELISA (ab197752) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse PAI1 (SERPINE1) ELISA Kit, Chemiluminescent (AB326886)
  • sELISA

Unknown

Sandwich ELISA - Mouse PAI1 (SERPINE1) ELISA Kit, Chemiluminescent (AB326886)

Interpolated concentrations of native PAI1 in mouse L929 cell culture supernatant samples treated with PMA and PHA for 3 days

Sandwich ELISA - Mouse PAI1 (SERPINE1) ELISA Kit, Chemiluminescent (AB326886)
  • sELISA

Unknown

Sandwich ELISA - Mouse PAI1 (SERPINE1) ELISA Kit, Chemiluminescent (AB326886)

Interpolated concentrations of PAI1 in mouse serum and plasma (citrate).

The concentrations of PAI1 were measured in duplicate and interpolated from the PAI1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAI1 concentration was determined to be 18.98 ng/mL in mouse serum and 7.69 ng/mL in mouse plasma (citrate).

Sandwich ELISA - Mouse PAI1 (SERPINE1) ELISA Kit, Chemiluminescent (AB326886)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PAI1 (SERPINE1) ELISA Kit, Chemiluminescent (AB326886)

Comparison of secreted PAI1 in unstimulated and PMA/PHA-stimulated L929 Cells.

L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. PAI1 was measured in 10-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the PAI1 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed : 17.9 ng/mL unstimulated, 31.1 ng/mL stimulated, and undetectable in media.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, Cell culture media, serum free media

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 43.37 pg/mL

Range

78.13 - 10000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse PAI1 (SERPINE1) ELISA Kit, Chemiluminescent (ab326886) is a rapid single-wash sandwich 55-min ELISA to measure Mouse PAI1 (SERPINE1) in Serum, Citrate plasma, Cell culture media, serum free media with a sensitivity of 43.37 pg/mL.

How the assay works

Mouse PAI1 (SERPINE1) ELISA Kit, Chemiluminescent (ab326886) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse PAI1 (SERPINE1) (ab326886) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Serpine1 antibody [EPR17272-22] - BSA and Aside free (Capture) ab242667
- Anti-Serpine1 antibody [EPR17272-29] - BSA and Aside free (Detector) ab242912
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.7" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 14.2" } ]
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Recovery

[ { "sample": "Serum", "range": "90 - 121 %", "average": "= 121" }, { "sample": "Citrate plasma", "range": "96 - 111 %", "average": "= 111" }, { "sample": "Cell culture media", "range": "72 - 88 %", "average": "= 88" }, { "sample": "serum free media", "range": "104 - 128 %", "average": "= 128" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair (By similarity). Involved in cellular and replicative senescence (PubMed : 16862142). Plays a role in alveolar type 2 cells senescence in the lung (PubMed : 28722352). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (By similarity).
See full target information Serpine1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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