Mouse PAI1 Total ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse PAI1 Total in Mouse, Rabbit in Plasma, Serum samples.
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair (By similarity). Involved in cellular and replicative senescence (PubMed:16862142). Plays a role in alveolar type 2 cells senescence in the lung (PubMed:28722352). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (By similarity).
Mr1, Pai1, Planh1, Serpine1, Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1
Mouse PAI1 Total ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse PAI1 Total in Mouse, Rabbit in Plasma, Serum samples.
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Sample 1 | n 20 | mean 0.288 ng/mL | SD 0.023 | C.V. 7.9 |
Sample Sample 2 | n 20 | mean 1.64 ng/mL | SD 0.081 | C.V. 4.92 |
Sample Sample 3 | n 20 | mean 20.8 ng/mL | SD 1.86 | C.V. 8.95 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Sample 1 | n 10 | mean 0.307 ng/mL | SD 0.04 | C.V. 12.9 |
Sample Sample 2 | n 10 | mean 1.46 ng/mL | SD 0.155 | C.V. 10.6 |
Sample Sample 3 | n 10 | mean 16.6 ng/mL | SD 2.41 | C.V. 14.6 |
Sample type | Average % | Range |
---|---|---|
Sample type Plasma | Average % = 100.8 | Range 89.1 - 112 % |
Abcam's Total PAI1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for is for the quantitative determination of active plasminogen activator inhibitor type 1 in mouse plasma.
Mouse PAI1 present in plasma reacts with the capture antibody coated and dried on a microtiter plate. Free, latent and complexed PAI1 will bind to the plate. Any unbound PAI1 is washed away and an anti-PAI1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the total PAI1 present in the samples, is then reacted with the secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of total PAI1 in the sample.
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PAI1 measured in biological fluids showing quantity (ng) per mL of tested sample. Samples diluted 3-81 fold.
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