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Mouse PAI1 Total ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse PAI1 Total in Mouse, Rabbit in Plasma, Serum samples.

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ELISA - Mouse PAI1 Total ELISA Kit (AB157529), expandable thumbnail
  • ELISA - Mouse PAI1 Total ELISA Kit (AB157529), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Mouse, Rabbit
Range
0.05 - 50 ng/mL
Assay time
1h 50m
Sensitivity
= 0.006 ng/mL

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Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair (By similarity). Involved in cellular and replicative senescence (PubMed:16862142). Plays a role in alveolar type 2 cells senescence in the lung (PubMed:28722352). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (By similarity).

Alternative names

What's included?

1 x 96 Tests
Components
10X Wash Buffer
1 x 50 mL
Anti-mouse PAI1 Primary Antibody
1 x 1 Vial
Anti-mouse PAI1 coated Microtiter Plate
1 x 1 Unit
Anti-rabbit horseradish peroxidase secondary antibody
1 x 10 µL
Mouse PAI1 Standard
1 x 1 Vial
TMB Substrate Solution
1 x 10 mL

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Mouse PAI1 Total ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse PAI1 Total in Mouse, Rabbit in Plasma, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Mouse, Rabbit
Range
0.05 - 50 ng/mL
Assay time
1h 50m
Assay Platform
Microplate
Sensitivity
= 0.006 ng/mL
General recovery
100.8%

Precision

Intra assay

Sample
Sample 1
n
20
mean
0.288 ng/mL
SD
0.023
C.V.
7.9
Sample
Sample 2
n
20
mean
1.64 ng/mL
SD
0.081
C.V.
4.92
Sample
Sample 3
n
20
mean
20.8 ng/mL
SD
1.86
C.V.
8.95

Inter assay

Sample
Sample 1
n
10
mean
0.307 ng/mL
SD
0.04
C.V.
12.9
Sample
Sample 2
n
10
mean
1.46 ng/mL
SD
0.155
C.V.
10.6
Sample
Sample 3
n
10
mean
16.6 ng/mL
SD
2.41
C.V.
14.6

Recovery

Sample specific recovery

Sample type
Plasma
Average %
= 100.8
Range
89.1 - 112 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Abcam's Total PAI1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for is for the quantitative determination of active plasminogen activator inhibitor type 1 in mouse plasma.

Mouse PAI1 present in plasma reacts with the capture antibody coated and dried on a microtiter plate. Free, latent and complexed PAI1 will bind to the plate. Any unbound PAI1 is washed away and an anti-PAI1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the total PAI1 present in the samples, is then reacted with the secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of total PAI1 in the sample.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • ELISA - Mouse PAI1 Total ELISA Kit (ab157529), expandable thumbnail

    ELISA - Mouse PAI1 Total ELISA Kit (ab157529)

    PAI1 measured in biological fluids showing quantity (ng) per mL of tested sample. Samples diluted 3-81 fold.

  • ELISA - Mouse PAI1 Total ELISA Kit (ab157529), expandable thumbnail

    ELISA - Mouse PAI1 Total ELISA Kit (ab157529)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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