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AB327047

Mouse PCSK9 ELISA Kit, Chemiluminescent

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Mouse PCSK9 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse PCSK9 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 2.29 - 5000 pg/ml with a sensitivity of 1.09 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
4 Images
Sandwich ELISA - Mouse PCSK9 ELISA Kit, Chemiluminescent (AB327047)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PCSK9 ELISA Kit, Chemiluminescent (AB327047)

Interpolated concentrations of native PCSK9 in mouse serum and plasmas and spiked recombinant mouse PCSK9 in cell culture supernatant.

The concentrations of PCSK9 were measured in duplicate interpolated from the PCSK9 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 0.25% plasma (citrate) 0.25% plasma (EDTA) 0.25% plasma (heparin) 0.13% and RAW 264.7 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean PCSK9 concentration was determined to be 353 ng/mL in serum 396 ng/mL in plasma (citrate) 348 ng/mL in plasma (EDTA) and 495 ng/mL in plasma (heparin). Mouse PCSK9 was not detected in unspiked 50% RAW 264.7 cell culture supernatant.

Sandwich ELISA - Mouse PCSK9 ELISA Kit, Chemiluminescent (AB327047)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PCSK9 ELISA Kit, Chemiluminescent (AB327047)

Interpolated concentrations of spiked recombinant PCSK9 in mouse liver extract and RAW 264.7 cell extract based on a 1000 µg/mL extract load.

The concentrations of PCSK9 were measured in duplicate interpolated from the PCSK9 standard curve and corrected for sample dilution. The interpolated dilution-factor-corrected values are plotted (mean +/- SD n=2). The mean native PCSK9 concentration was determined to be 2.36 ng/mg in liver extract and it was undetected in RAW 264.7 cell extract.

Sandwich ELISA - Mouse PCSK9 ELISA Kit, Chemiluminescent (AB327047)
  • sELISA

Lab

Sandwich ELISA - Mouse PCSK9 ELISA Kit, Chemiluminescent (AB327047)

Standard curve comparison between the Chemiluminescent Mouse PCSK9 SimpleStep ELISA and colorimetric Mouse PCSK9 SimpleStep ELISA (colorimetric ab215538) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse PCSK9 ELISA Kit, Chemiluminescent (AB327047)
  • sELISA

Lab

Sandwich ELISA - Mouse PCSK9 ELISA Kit, Chemiluminescent (AB327047)

Dynamic range comparison between the Chemiluminescent Mouse PCSK9 SimpleStep ELISA, colorimetric Mouse PCSK9 SimpleStep ELISA (colorimetric ab215538), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Serum, EDTA Plasma, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.09 pg/mL

Range

2.29 - 5000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse PCSK9 ELISA Kit, Chemiluminescent (ab327047) is a rapid single-wash sandwich 55-min ELISA to measure Mouse PCSK9 in Cell culture supernatant, Serum, EDTA Plasma, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma with a sensitivity of 1.09 pg/mL.

How the assay works

Mouse PCSK9 ELISA Kit, Chemiluminescent (ab327047) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse PCSK9 (ab327047) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Proexpress Convertase 9 ( Pcsk9) antibody [EPR17827-111] - BSA and Aside free (Capture) ab242601
- Anti-Proexpress Convertase 9 ( Pcsk9) antibody [EPR17827-117] - BSA and Aside free (Detector) ab242847

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.4" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "88 - 105 %", "average": "= 98" }, { "sample": "Serum", "range": "102 - 107 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "101 - 102 %", "average": "= 101" }, { "sample": "Cell culture extracts", "range": "128 - 130 %", "average": "= 129" }, { "sample": "Tissue Extracts", "range": "99 - 105 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "106 - 114 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "95 - 103 %", "average": "= 99" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members : low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
See full target information Pcsk9

Alternative Names

Narc1, Pcsk9, Proprotein convertase subtilisin/kexin type 9, Neural apoptosis-regulated convertase 1, Proprotein convertase 9, Subtilisin/kexin-like protease PC9, NARC-1, PC9
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com