Mouse PD-1H ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse PD-1H with a sensitivity of 44.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 390.63 - 25000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 44.3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Immunoregulatory receptor which inhibits the T-cell response (PubMed:21383057, PubMed:24743150, PubMed:25267631). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (PubMed:20042595). May stimulate MMP14-mediated MMP2 activation (By similarity).
Alternative names
Dies1, PD-1H, VISTA, Vsir, V-type immunoglobulin domain-containing suppressor of T-cell activation, Platelet receptor Gi24, V-set domain-containing immunoregulatory receptor, V-set immunoregulatory receptor
What's included?
Recommended products
Mouse PD-1H ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse PD-1H with a sensitivity of 44.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 390.63 - 25000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 44.3 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 9 | mean - | SD - | C.V. 2.9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 2.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 120 | Range 113 - 123 % |
Sample type EDTA Plasma | Average % = 117 | Range 108 - 123 % |
Sample type Tissue Extracts | Average % = 111 | Range 103 - 118 % |
Sample type Heparin Plasma | Average % = 109 | Range 105 - 123 % |
Sample type Cell Lysate | Average % = 106 | Range 102 - 110 % |
Sample type Citrate plasma | Average % = 107 | Range 103 - 108 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse PD-1H ELISA Kit (ab231933) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PD-1H protein in cit plasma, edta plasma, hep plasma, serum, tissue extracts, and cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PD-1H with 44.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VISTA also known as V-domain Ig suppressor of T cell activation or Gi24 is a protein significant in immune regulation. It is a type I transmembrane glycoprotein with an apparent mass of approximately 55 kDa. VISTA is expressed on hematopoietic cells specifically within immune system components such as myeloid cells and T cells. Researchers have identified biotinylated chimeric proteins which facilitate the study of VISTA's expression and interactions in various immunological research models.
- Biological function summary
VISTA functions in immune checkpoint regulation by modulating T cell activity. It acts as both a ligand and a receptor influencing immune responses. As part of an immune complex VISTA interacts with other checkpoint proteins to maintain immune homeostasis and prevent autoimmunity. Its role mirrors those of other B7 family members like PD-1 and CTLA-4 which are involved in negative immune regulation.
- Pathways
VISTA integrates into the broader framework of immune signaling pathways. It contributes significantly to the immune checkpoint pathway similar to CTLA-4 and PD-1 which mediate immune cell proliferation and activation. By interacting with these proteins VISTA plays an important role in maintaining self-tolerance and regulating inflammatory responses functioning parallel to other proteins such as CD28 and B7-H5 in the immune checkpoint pathway.
- Associated diseases and disorders
VISTA's improper regulation associates with autoimmune diseases and cancer. Its expression influences the progression of conditions like rheumatoid arthritis where it modulates immune responses and inflammation. Additionally in oncology VISTA acts as an inhibitory molecule; hence targeting it can enhance anti-tumor immunity similar to therapies targeting proteins like PD-1 and PD-L1. Understanding VISTA's intricate connections helps in developing therapies for these diseases.
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3 product images
Sandwich ELISA - Mouse PD-1H ELISA Kit (ab231933)
Example of mouse PD-1H standard curve in 1X Cell Extraction Buffer PTR.
The PD-1H standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse PD-1H ELISA Kit (ab231933)
Interpolated concentrations of native PD-1H in mouse serum and plasma samples.
The concentrations of PD-1H were measured in duplicates, interpolated from the PD-1H standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 100%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-1H concentration in the neat samples was determined to be 19.7 ng/mL in serum, 17.8 ng/mL in plasma (citrate), 15.3 ng/mL in plasma (EDTA), and 31.8 ng/mL in plasma (heparin).
Sandwich ELISA - Mouse PD-1H ELISA Kit (ab231933)
Interpolated concentrations of native PD-1H in mouse spleen extract, lung extract, placenta extract, and RAW 264.7 cell extract.
The concentrations of PD-1H were measured in duplicate and interpolated from the PD-1H standard curve and corrected for sample dilution. Undiluted samples are 250 μg/mL for all extracts. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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