AB283880

Mouse PD-L2 ELISA Kit

Recombinant
SimpleStep
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Mouse PD-L2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse PD-L2 with a sensitivity of 1.03 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD273, B7dc, Btdc, Cd273, Pdl2, Pdcd1lg2, Programmed cell death 1 ligand 2, PD-1 ligand 2, PD-L2, PDCD1 ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, B7-DC

2 Images

Sandwich ELISA - Mouse PD-L2 ELISA Kit (AB283880)

sELISA

Supplier Data

Sandwich ELISA - Mouse PD-L2 ELISA Kit (AB283880)

Interpolated concentrations of native PD-L2 in mouse serum, plasma, and urine samples.

The concentrations of PD-L2 were measured in duplicates, interpolated from the PD-L2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 100%, plasma (EDTA) 100%, plasma (heparin) 100% and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L2 concentration was determined to be 699.03 pg/mL in serum, 272.41 pg/mL in plasma (citrate), 310.34 pg/mL in plasma (EDTA), 412.41 pg/mL in plasma (heparin) and 47.99 pg/mL in urine.

sELISA

Supplier Data

Sandwich ELISA - Mouse PD-L2 ELISA Kit (AB283880)

Example of mouse PD-L2 standard curve in Sample Diluent NS.

The PD-L2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 1.03 pg/mL

Range

5.47 - 350 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse PD-L2 ELISA Kit (ab283880) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PD-L2 protein in serum, plasma and urine samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PD-L2 with 1.03 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Mouse PD-L2 is involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" } ]

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Recovery

[ { "sample": "Serum", "range": "95 - 103 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "97 - 106 %", "average": "= 102" }, { "sample": "Urine", "range": "85 - 97 %", "average": "= 92" }, { "sample": "Heparin Plasma", "range": "105 - 113 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "98 - 105 %", "average": "= 102" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PD-L2 also known as PDCD1LG2 or B7-DC is a member of the B7 protein family that plays a role in immune system regulation. This protein has a molecular mass of approximately 25 kDa. PD-L2 is expressed in antigen-presenting cells such as dendritic cells and macrophages. Expression is also located in some subsets of T cells and B cells. The PD-L2 can act as a second ligand for the PD-1 receptor which is important in immune checkpoints.

Biological function summary

PD-L2 offers protection to tissues from autoimmunity by regulating T-cell activation and fostering tolerance. By engaging with its receptor PD-1 PD-L2 forms a complex that modulates the immune response to prevent overactivation. This interaction limits the immune system's potential damage to healthy tissues maintaining proper immune function in diverse environments.

Pathways

PD-L2 participates in the immune checkpoint pathways where it works closely with PD-1 to mediate immune responses. PD-L2 forms an important part of the program death-1 (PD-1) pathway which plays a prominent role in downregulating T-cell activity. It relates to proteins such as PD-L1 which also interacts with PD-1 modulating immune tolerance and response to infections or tumors.

PD-L2 involvement has been significant in cancer immunology and autoimmune conditions. Its interaction with PD-1 relates to the suppression of immune responses in tumors contributing to cancer progression. PD-L2's function as an immune checkpoint ligand associates with autoimmune diseases like rheumatoid arthritis where immune regulation goes awry. Both PD-1 and PD-L2 represent potential targets for therapeutic interventions due to their roles in these conditions.

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Product protocols

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Target data

Involved in the costimulatory signal essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

See full target information Pdcd1lg2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse PD-L2 ELISA Kit

Sandwich ELISA - Mouse PD-L2 ELISA Kit (AB283880)

Sandwich ELISA - Mouse PD-L2 ELISA Kit (AB283880)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Assay Kits

Assay Kits

Secondary Antibodies

Product promise