AB210971

Mouse PD1 ELISA Kit

Recombinant
SimpleStep
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(2 Publications)

Mouse PD1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse PD1 with a sensitivity of 21 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD279, Pd1, Pdcd1, Programmed cell death protein 1, Protein PD-1, mPD-1

5 Images

Sandwich ELISA - Mouse PD1 ELISA Kit (AB210971)

sELISA

Supplier Data

Sandwich ELISA - Mouse PD1 ELISA Kit (AB210971)

Comparison of PD1 in unstimulated and PHA/PMA (1.5% and 10ng/mL respectively) stimulated EL-4 supernatant.

EL-4 cells were grown in the absence or presence of PHA/PMA for 48 hours. The concentrations of PD1 were measured in neat supernatant samples in duplicates and interpolated from the PD1 standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean PD1 concentration was determined to be 32 pg/mL in unstimulated, 203 pg/ml in PHA/PMA stimulated supernatants and undetectable in media (not shown).

sELISA

Supplier Data

Sandwich ELISA - Mouse PD1 ELISA Kit (AB210971)

Example of mouse PD1 standard curve 1X Cell Extraction Buffer PTR+Enhancer.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse PD1 ELISA Kit (AB210971)

Interpolated concentrations of native PD1 in PHA/PMA treated Day 2 EL-4 cell culture supernatant samples.

The concentrations of PD1 were measured in duplicates, interpolated from the PD1 standard curves and corrected for sample dilution. Undiluted samples are as follows : EL-4 cell culture supernatant 100%. Dilutions beyond 4 fold were not analyzed due to O.D. values below the O.D. values of the MDD. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD1 concentration was determined to be 195 pg/mL in EL-4 cell culture supernatant samples.

sELISA

Supplier Data

Sandwich ELISA - Mouse PD1 ELISA Kit (AB210971)

Example of mouse PD1 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Mouse PD1 ELISA Kit (AB210971)

Interpolated concentrations of native PD1 in mouse EL-4 cell extract samples based on a 90 μg/mL extract load.

The concentrations of PD1 were measured in duplicate and interpolated from the PD1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD1 concentration was determined to be 4,246 pg/mL in EL-4 cell extract.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Cell culture supernatant

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 21 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse PD1 ELISA Kit (ab210971) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PD1 protein in cell culture extracts, cell culture supernatant, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PD1 with 21 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

PD1 is a single-pass type I cell membrane glycoprotein. It is a receptor for PD-1L and/or PD-L2 ligands. PD1 is involved in the regulation of T-cell function during immunity and tolerance. PD-1 interactions with its ligands PD-L1 and/or PD-L2 provides a negative regulatory signal to CD4 and CD8 T cells that results ultimately in a phenotype termed T cell exhaustion. Possible cell death inducer, in association with other factors.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "EL-4 Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" }, { "reproducibilityType": "Intra", "sample": "EL-4 Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 104 %", "average": "= 100" }, { "sample": "Cell culture extracts", "range": "100 - 115 %", "average": "= 109" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab210971-1x96Tests", "assetComponentDetails": [ { "size":"1 x 500 µL", "name":"Denaturant", "number":"AB210971-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB210971-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB210971-CMP14", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse PD-1 Capture Antibody", "number":"AB210971-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB210971-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse PD-1 Lyophilized Recombinant Protein", "number":"AB210971-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB210971-CMP13", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB210971-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse PD-1 Detector Antibody", "number":"AB210971-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB210971-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB210971-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB210971-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB210971-CMP10", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PD1 also known as Programmed Cell Death Protein 1 or PDCD1 is a transmembrane protein that plays a critical role in regulating immune responses. It has a mass of approximately 55 kDa. PD1 is expressed on the surface of T cells B cells and some myeloid cells. PD1’s expression increases upon activation of these immune cells assisting in maintaining peripheral tolerance. Researchers often use PD1 mouse models and chimeric antibodies to explore the function of PD1 for experimental purposes. Antibodies such as anti-PD1 such as EH12.2H7 help in blocking PD1 interaction to study its role further.

Biological function summary

PD1 serves as an inhibitory receptor acting as a checkpoint in the immune system. It becomes part of an immune-suppressive complex when it binds with its ligands PD-L1 or PD-L2 which are expressed on various cell types including some tumor cells. This interaction suppresses the proliferation of T cells and cytokine production contributing to immune homeostasis. By controlling T cell activity PD1 limits autoimmunity but can also reduce the immune system's capability to attack cancer cells.

Pathways

PD1 functions in the immune checkpoint pathway a critical regulatory circuit in immune regulation. The engagement of PD1 with its ligands initiates a cascade that inhibits the function and proliferation of T cells through downstream SHP-2 phosphatase activity. This pathway frequently involves other regulatory proteins like CTLA-4 and is an important mechanism by which the body modulates immune responses. Related pathways often intersect with those involving T cell receptor signaling and contribute to the overall modulation of immune activity.

PD1 has a significant role in cancer and autoimmune disorders. PD1 expression can allow tumors to evade immune surveillance making PD1 a target for cancer therapies such as anti-PD1 antibodies which aim to block PD1 and restore T cell activity. The interaction of PD1 with cancer-related proteins like PD-L1 facilitates tumor immune evasion. In autoimmune disorders PD1’s regulation of immune balance can become dysregulated leading to persistent immune activation and tissue damage. Understanding PD1 and its interaction with proteins such as PD-L1 helps in developing therapeutic strategies for both cancer and autoimmune conditions.

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Product protocols

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Target data

Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 10485649, PubMed : 11209085, PubMed : 11698646, PubMed : 21300912). Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed : 11015443, PubMed : 11224527, PubMed : 18287011, PubMed : 18641123, PubMed : 22641383). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (PubMed : 22641383). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2 : following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (PubMed : 11698646, PubMed : 22641383). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (By similarity).

See full target information Pdcd1

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Publications (2)

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse PD1 ELISA Kit

Sandwich ELISA - Mouse PD1 ELISA Kit (AB210971)

Sandwich ELISA - Mouse PD1 ELISA Kit (AB210971)

Sandwich ELISA - Mouse PD1 ELISA Kit (AB210971)

Sandwich ELISA - Mouse PD1 ELISA Kit (AB210971)

Sandwich ELISA - Mouse PD1 ELISA Kit (AB210971)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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