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AB267589

Mouse PDGF AB ELISA Kit

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Mouse PDGF AB ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse PDGF AB in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, Pdgfa

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Sandwich ELISA - Mouse PDGF AB ELISA Kit (AB267589)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PDGF AB ELISA Kit (AB267589)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 26 pg/mL

Range

24.58 - 6000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse PDGF AB ELISA Kit is designed for the quantitative determination of PDGF AB in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for mouse PDGF AB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF AB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse PDGF AB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF AB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit detects mouse and human PDGF AB.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "128 - 141 %", "average": "= 133.5" }, { "sample": "Plasma", "range": "133 - 148 %", "average": "= 139.3" }, { "sample": "Cell culture media", "range": "119 - 134 %", "average": "= 127.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267589-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267589-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267589-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"450X HRP-Streptavidin Concentrate", "number":"AB267589-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267589-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267589-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse PDGF AB Standard (Lyophilized)", "number":"AB267589-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Mouse PDGF AB Detection Antibody", "number":"AB267589-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse PDGF AB coated Microplate (12 x 8 wells)", "number":"AB267589-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDGF AB also known as Platelet-Derived Growth Factor AB is a heterodimeric protein with an approximate mass around 30 kDa. The PDGF AB protein plays an important role in cell proliferation and differentiation by activating its receptor PDGFR. It gets expressed in a variety of cell types including fibroblasts smooth muscle cells and endothelial cells. This growth factor influences cellular behaviors through its interactions with cells in connective tissues where it shows significant levels of expression.
Biological function summary

The PDGF AB protein promotes cellular proliferation and migration particularly in the context of wound healing and development. It forms part of a larger family of PDGF molecules that include homodimers and heterodimers and it requires specific receptor binding to exert its function. As a member of the growth factor family PDGF AB contributes to the signaling pathway that regulates tissue growth and repair processes.

Pathways

PDGF AB fits significantly into the PI3K/AKT and MAPK signaling pathways. These pathways are key in regulating cellular growth and survival and they involve interactions with important proteins like Ras and AKT. The activity of PDGF AB in these pathways affects various downstream molecular processes making it an integral component of cellular signaling networks.

PDGF AB connects to a few pathologies notably atherosclerosis and certain forms of cancer. It often associates with diseases where aberrant cell proliferation and migration occur. For instance in atherosclerosis PDGF AB interacts with proteins such as Integrins and MMPs which contribute to the remodeling of blood vessels. Similarly in oncology the dysregulation of PDGF AB can lead to malignancies with its interaction through growth-promoting pathways.
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Product protocols

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Target data

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB.
See full target information Pdgfa

Additional targets

Pdgfb
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websiteProtocolBooklet
en
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