AB224879

Mouse PDGF BB ELISA Kit

Recombinant
SimpleStep
What is this?

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(4 Publications)

Mouse PDGF BB ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse PDGF BB with a sensitivity of 1.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Sis, Pdgfb, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis

7 Images

Sandwich ELISA - Mouse PDGF BB ELISA Kit (AB224879)

sELISA

Lab

Sandwich ELISA - Mouse PDGF BB ELISA Kit (AB224879)

Interpolated concentration of native PDGF BB was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 4% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Mouse PDGF BB ELISA Kit (AB224879)

Example of mouse PDGF BB standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse PDGF BB ELISA Kit (AB224879)

Interpolated concentrations of native PDGF BB in mouse serum and plasma samples.

The concentrations of PDGF BB were measured in duplicates, interpolated from the PDGF BB standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 4%, plasma (citrate) 25%, plasma (heparin) 25% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGF BB concentration was determined to be 9591 pg/mL in neat serum, 976.9 pg/mL in neat plasma (citrate), 555.5 pg/mL in neat plasma (heparin), and 1773 pg/mL in neat plasma (EDTA).

sELISA

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Sandwich ELISA - Mouse PDGF BB ELISA Kit (AB224879)

Mouse PDGF BB Competitor standard curve comparison.

Standard curve comparison between Mouse PDGF BB SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Lab

Sandwich ELISA - Mouse PDGF BB ELISA Kit (AB224879)

Example of mouse PDGF BB standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse PDGF BB ELISA Kit (AB224879)

Other species reactivity was determined by measuring 4% rat serum samples, interpolating the protein concentrations from the mouse standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in mouse serum assayed at the same dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGF BB concentration was determined to be 10,988 pg/mL in neat mouse serum and 1368.2 pg/mL in neat rat serum. Reactivity with rat serum was determined to be 12%.

sELISA

Supplier Data

Sandwich ELISA - Mouse PDGF BB ELISA Kit (AB224879)

Interpolated concentrations of native PDGF BB in mouse LPS stimulated J774A.1 cell culture supernatant (72 hours) samples.

The concentrations of PDGF BB were measured in duplicates, interpolated from the PDGF BB standard curves and corrected for sample dilution. Undiluted samples are as follows : J774A.1 cell culture supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGF BB concentration was determined to be 3882 pg/mL in neat J774A.1 cell culture supernatant.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 1.9 pg/mL

Range

9.375 - 600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse PDGF BB ELISA Kit (ab224879) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PDGF BB protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PDGF BB with 1.9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

PDGF B (Platelet derived growth factor B) plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. As a homodimer it is a potent mitogen for cells of mesenchymal origin and is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. PDGF-BB is essential for normal blood vessel development, for normal development of kidney glomeruli and in wound healing. Signaling is modulated by the formation of heterodimers with PDGF A chain to yield PDGF-AB.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]

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Recovery

[ { "sample": "Serum", "range": "104 - 115 %", "average": "= 111" }, { "sample": "EDTA Plasma", "range": "94 - 111 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "97 - 106 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "104 - 115 %", "average": "= 109" }, { "sample": "Cell culture media", "range": "91 - 93 %", "average": "= 92" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDGF-B also known as platelet-derived growth factor subunit B or PDGF-BB is a critical protein involved in various cellular processes. Composed of 241 amino acids PDGF-B exhibits a molecular mass of approximately 27 kDa. Scientists recognize this protein for its expression primarily in platelets but it also appears in a range of other tissues including smooth muscle cells and fibroblasts. The PDGF-B protein plays a substantial role in the regulation of growth and division inducing pathways important for cellular proliferation.

Biological function summary

PDGF-B influences cellular signaling processes and facilitates interactions that are a part of the PDGF receptor system. PDGF-B can form a homo-dimerization commonly known as the PDGF-BB dimer which is an active form. When PDGF-B binds to its receptor specifically the PDGF receptor β (PDGFR-β) it triggers pathways that control various cellular activities including growth and survival. The interaction of PDGF-B with the receptor is an important step that modulates several downstream signaling pathways.

Pathways

PDGF-B activates significant pathways such as the MAPK/ERK and PI3K/AKT pathways. These pathways play a major role in the regulation of cell proliferation and differentiation. Through these pathways PDGF-B exhibits interactions with proteins like Ras and Akt which are integral in transmitting signals within the cell. These cascades result in the promotion of processes such as angiogenesis and wound healing highlighting the importance of PDGF-B in cellular function.

Researchers have linked PDGF-B to pathologies such as cancer and atherosclerosis. For instance overexpression of PDGF-B can lead to abnormal vascular growth contributing to tumor development in cancers. It also plays a role in the progression of atherosclerosis by promoting the migration and proliferation of smooth muscle cells leading to plaque formation. In these contexts PDGF-B shows interaction with other proteins such as VEGF in tumor angiogenesis and LDL in atherosclerotic developments reinforcing its involvement in these conditions.

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Product protocols

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Target data

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.

See full target information Pdgfb

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation (London, England) 22:11 PubMed40033399

2025

Contribution of brain pericytes to neuroinflammation following repetitive head trauma.

Applications

Unspecified application

Species

Unspecified reactive species

Arianna Cembran,Maxwell Eisenbaum,Daniel Paris,Michael Mullan,Fiona Crawford,Scott Ferguson,Corbin Bachmeier

Open life sciences 18:20220670 PubMed38239497

2024

Fractalkine modulates pulmonary angiogenesis and tube formation by modulating CX3CR1 and growth factors in PVECs.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Liao,Xianwu Yang,Jiejie Yang,Jingjing Xiao,Xuyang Liu,Yingquan Zhuo,Jiafei Yang,Huajian Gu

American journal of physiology. Gastrointestinal and liver physiology 321:G378-G388 PubMed34405729

2021

Nrf2 expression in pancreatic stellate cells promotes progression of cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Tanaka,Shin Hamada,Ryotaro Matsumoto,Keiko Taguchi,Masayuki Yamamoto,Atsushi Masamune

Science advances 6: PubMed33208358

2020

Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqun Li,Lipeng Wang,Biaotong Huang,Yanqiu Gu,Ying Luo,Xin Zhi,Yan Hu,Hao Zhang,Zhengrong Gu,Jin Cui,Liehu Cao,Jiawei Guo,Yajun Wang,Qirong Zhou,Hao Jiang,Chao Fang,Weizong Weng,Xiaofei Chen,Xiao Chen,Jiacan Su

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse PDGF BB ELISA Kit

Sandwich ELISA - Mouse PDGF BB ELISA Kit (AB224879)

Sandwich ELISA - Mouse PDGF BB ELISA Kit (AB224879)

Sandwich ELISA - Mouse PDGF BB ELISA Kit (AB224879)

Sandwich ELISA - Mouse PDGF BB ELISA Kit (AB224879)

Sandwich ELISA - Mouse PDGF BB ELISA Kit (AB224879)

Sandwich ELISA - Mouse PDGF BB ELISA Kit (AB224879)

Sandwich ELISA - Mouse PDGF BB ELISA Kit (AB224879)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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