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Mouse PDGFR beta ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse PDGFR beta in Cell culture media, Cell culture supernatant, Serum, EDTA Plasma samples.

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Images

Sandwich ELISA - Mouse PDGFR beta ELISA Kit (AB253225), expandable thumbnail
  • Sandwich ELISA - Mouse PDGFR beta ELISA Kit (AB253225), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Mouse

Range

109.4 - 7000 pg/mL

Assay time

1h 30m

Sensitivity

= 20.3 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM (By similarity). Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.

Alternative names

What's included?

1 x 96 Tests
Components
10X Mouse PDGFR beta Capture Antibody
1 x 600 µL
10X Mouse PDGFR beta Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent 4BI
1 x 6 mL
Mouse PDGFR beta Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Mouse PDGFR beta ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse PDGFR beta in Cell culture media, Cell culture supernatant, Serum, EDTA Plasma samples.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Mouse

Range

109.4 - 7000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 20.3 pg/mL

Precision

Intra assay

Sample

Serum

n

8

mean

-

SD

-

C.V.

3.3

Inter assay

Sample

Serum

n

3

mean

-

SD

-

C.V.

2.6

Recovery

Sample specific recovery

Sample type

Cell culture supernatant

Average %

= 105

Range

104 - 107 %

Sample type

Serum

Average %

= 93

Range

90 - 95 %

Sample type

EDTA Plasma

Average %

= 112

Range

106 - 116 %

Sample type

Cell culture media

Average %

= 104

Range

103 - 106 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Mouse PDGFR beta ELISA Kit (ab253225) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PDGFR beta protein in cell culture media, cell culture supernatant, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PDGFR beta with 20.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

PDGFR beta (Platelet-derived growth factor receptor beta) is a tyrosine kinase receptor for members of the platelet derived growth factor family, homodimeric PDGFB and PDGFD, and heterodimeric PDGFA/B. Binding plays an essential role in embryonic development, blood vessel development, and cell proliferation, migration, survival, chemotaxis, and differentiation.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PDGFR beta also known as PDGFR-b or PDGFRB is a cell surface tyrosine kinase receptor with a molecular mass of about 180 kDa. It binds the PDGF (platelet-derived growth factor) family of ligands. PDGFR beta is commonly found in various tissues including blood vessels and connective tissues and is highly expressed in cells like pericytes and fibroblasts. The receptor plays a critical role in cell signaling mechanisms involving proliferation chemotaxis and survival.

Biological function summary

The receptor plays an essential role in the regulation of cell growth and development. PDGFR beta undergoes dimerization and autophosphorylation upon ligand binding initiating a series of downstream signaling cascades. This receptor is often part of a complex with other receptor proteins promoting interactions necessary for signal propagation. Its main biological functions include mediating cellular responses to environmental signals that contribute to tissue repair and angiogenesis.

Pathways

PDGFR beta is an important player within the PI3K-Akt and MAPK signaling pathways. It works alongside proteins such as PI3K and Ras to regulate cellular responses related to growth and survival. These pathways facilitate cross-talk with other cellular processes influencing various cellular outcomes. This receptor's activity regulates critical physiological functions by providing signals that maintain cellular homeostasis under various physiological conditions.

Associated diseases and disorders

PDGFR beta has significant implications in the progression of cancer and fibrotic diseases. Its overexpression or mutation can lead to anomalous signaling that contributes to tumorigenesis particularly in connective tissue tumors known as sarcomas. Additionally PDGFR beta's role in promoting fibroblast activity makes it relevant in fibrotic diseases such as pulmonary fibrosis. Abnormal activation of PDGFR beta can interact with related proteins like VEGF receptors enhancing pathogenic responses and contributing to disease severity.

Product promise

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2 product images

  • Sandwich ELISA - Mouse PDGFR beta ELISA Kit (ab253225), expandable thumbnail

    Sandwich ELISA - Mouse PDGFR beta ELISA Kit (ab253225)

    Example of mouse PDGFR beta standard curve in Sample Diluent NS.

    The PDGFR beta standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Mouse PDGFR beta ELISA Kit (ab253225), expandable thumbnail

    Sandwich ELISA - Mouse PDGFR beta ELISA Kit (ab253225)

    Interpolated concentrations of native PDGFR beta in mouse serum, plasma (EDTA), and cell culture supernatant samples.

    The concentrations of PDGFR beta were measured in duplicates, interpolated from the PDGFR beta standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (EDTA) 12.5%, NIH/3T3 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGFR beta concentration was determined to be 28.59 ng/mL in neat serum, 22.71 ng/mL in neat plasma (EDTA), and 0.578 ng/mL in neat NIH/3T3 cell culture supernatant.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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