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AB215544

Mouse PDK4 ELISA Kit

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(1 Publication)

Mouse PDK4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse PDK4 with a sensitivity of 45 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Pyruvate dehydrogenase kinase isoform 4, Pdk4

5 Images
Sandwich ELISA - Mouse PDK4 ELISA Kit (AB215544)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PDK4 ELISA Kit (AB215544)

Example of mosue PDK4 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse PDK4 ELISA Kit (AB215544)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PDK4 ELISA Kit (AB215544)

Interpolated concentrations of PDK4 in various cell and tissue extracts. The concentrations of PDK4 were measured in three different dilutions in duplicate and interpolated from the PDK4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng PDK4 per mg of extract (mean +/- SD, n=3). PDK4 concentration was determined to be 9.9 ng/mg in C2C12 cell extract, 17.3 ng/mg in mouse skeletal muscle extract, 26.7 ng/mg in mouse heart, and 36.7 ng/mg in rat heart.

Sandwich ELISA - Mouse PDK4 ELISA Kit (AB215544)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PDK4 ELISA Kit (AB215544)

Interpolated concentrations of PDK4 in various species heart tissue extracts.

Other species reactivity was determined by measuring a 300 μg/mL extract load of various species heart tissue extract, and interpolating the protein concentrations from the mouse standard curve.

Sandwich ELISA - Mouse PDK4 ELISA Kit (AB215544)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PDK4 ELISA Kit (AB215544)

Interpolated concentrations of native PDK4 in C2C12 cell extract sample, mouse skeletal muscle tissue extract and mouse and rat heart tissue extracts.

Interpolated concentrations of native PDK4 in C2C12 cell extract sample based on a 500 μg/mL extract load, mouse skeletal muscle tissue extract based on a 500 μg/mL extract load, and mouse and rat heart tissue extract based on a 300 μg/mL extract load. The concentrations of PDK4 were measured in duplicate and interpolated from the PDK4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDK4 concentration was determined to be 4.8 ng/mL in C2C12 extract, 8.4 ng/mL in mouse skeletal muscle extract, 7.8 ng/mL in mouse heart extract, and 10.5 ng/mL in rat heart extract.

Sandwich ELISA - Mouse PDK4 ELISA Kit (AB215544)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PDK4 ELISA Kit (AB215544)

Representative image of Mouse PDK4 ELISA Kit (ab215544)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Mouse PDK4 ELISA Kit Capture Antibody

- 10X Mouse PDK4 Detector Antibody

- 10X Mouse PDK4 ELISA Kit Lyophilised Purified Protein (2 vials)

- Mouse PDK4 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 45 pg/mL

Range

0.234 - 15 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse PDK4 ELISA Kit (ab215544) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PDK4 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PDK4 with 45 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

PDK4 is a mitochondrial kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. PDH phosphorylation inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. PDK4 plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, PDK4 plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, PDK4 mediates cellular responses to glucose levels and to a high-fat diet. PDK4 regulates both fatty acid oxidation and de novo fatty acid biosynthesis. PDK4 plays a role in the generation of reactive oxygen species.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "94 - 103 %", "average": "= 98" }, { "sample": "Mouse muscle extract", "range": "95 - 100 %", "average": "= 98" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab215544-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB215544-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB215544-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB215544-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB215544-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB215544-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB215544-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse PDK4 Lyophilized Recombinant Protein", "number":"AB215544-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB215544-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse PDK4 Capture Antibody", "number":"AB215544-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse PDK4 Detector Antibody", "number":"AB215544-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB215544-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB215544-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDK4 also known as Pyruvate Dehydrogenase Kinase Isozyme 4 plays an essential role in regulating mitochondrial energy metabolism. PDK4 phosphorylates the pyruvate dehydrogenase complex controlling the transformation of pyruvate to acetyl-CoA a critical step in energy production. The protein has a molecular weight of around 49 kDa. PDK4 expression occurs in various tissues with high levels observed in skeletal muscle heart and liver reflecting its role in energy metabolic processes.
Biological function summary

The enzyme functions to inhibit the pyruvate dehydrogenase complex by adding phosphate groups which reduces the conversion of pyruvate to acetyl-CoA and shifts energy production from carbohydrates to fats especially during fasting or prolonged exercise. PDK4 operates as a homodimer and is often part of a larger regulatory system balancing glucose and fatty acid oxidation in response to nutrient availability.

Pathways

PDK4 is integral to the regulation of both glucose metabolism and fatty acid oxidation. The enzyme interacts closely with the insulin signaling pathway where it modulates glucose uptake and utilization based on hormonal signals. Additionally PDK4 is a part of the hypoxia-inducible factor-1 (HIF-1) pathway where it plays a role in cellular response to low oxygen levels interacting with related proteins such as pyruvate dehydrogenase (PDH).

PDK4's activity is linked to metabolic conditions like diabetes and obesity where its altered expression can lead to insulin resistance by affecting glucose utilization. Furthermore PDK4 is associated with cardiovascular diseases through its influence on cardiac energy metabolism and its interaction with other proteins such as PDH contributing to heart dysfunction when deregulated.
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Product protocols

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Target data

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.
See full target information Pdk4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Saudi journal of biological sciences 28:1966-1977 PubMed33732083

2021

Short-term administration of stimulates insulin signaling, suppresses fatty acids metabolism, and increases glucose uptake and utilization in the hearts of healthy rats.

Applications

Unspecified application

Species

Unspecified reactive species

Abdullah S Shatoor,Suliman Al Humayed,Hussain M Almohiy
View all publications
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websiteProtocolBooklet
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