AB100735

Mouse PF4 ELISA Kit (CXCL4)

Name: Mouse PF4 ELISA Kit (CXCL4) (ab100735) | Abcam
Brand: Abcam Limited
SKU: AB100735
Price: 780 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(2 Publications)

Mouse PF4 ELISA Kit (CXCL4) is a sandwich ELISA designed to quantify Mouse PF4 (CXCL4) with a sensitivity of 6 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 4.12 - 3000 pg/mL

View Alternative Names

Cxcl4, Scyb4, Pf4, Platelet factor 4, PF-4, C-X-C motif chemokine 4

4 Images

Sandwich ELISA - Mouse PF4 ELISA Kit (CXCL4) (AB100735)

sELISA

Unknown

Sandwich ELISA - Mouse PF4 ELISA Kit (CXCL4) (AB100735)

Representative standard curve using ab100735

ELISA

Lab

ELISA - Mouse PF4 ELISA Kit (CXCL4) (AB100735)

PF4 measured in cell supernatanses showing quantity (pg) per mL tested sample

sELISA

Unknown

Sandwich ELISA - Mouse PF4 ELISA Kit (CXCL4) (AB100735)

Representative standard curve using ab100735

ELISA

Lab

ELISA - Mouse PF4 ELISA Kit (CXCL4) (AB100735)

PF4 measured in biofluids showing quantity (microgram) per mL of tested sample

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 6 pg/mL

Range

4.12 - 3000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse PF4 ELISA Kit (CXCL4) ab100735 is a sandwich ELISA to measure Mouse PF4 (CXCL4) in serum, plasma, cell culture supernatant with a sensitivity of 6 pg/ml.

How the assay works

This assay employs an antibody specific for mouse PF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and PF4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse PF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PF4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse PF4 ELISA Kit (CXCL4) ab100735 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "67 - 83 %", "average": "= 73.99" }, { "sample": "Serum", "range": "104 - 127 %", "average": "= 118.2" }, { "sample": "Plasma", "range": "92 - 129 %", "average": "= 109.3" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100735-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100735-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100735-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse PF4 Standard (lyophilized)", "number":"AB100735-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100735-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB100735-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse PF4", "number":"AB100735-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100735-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100735-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"PF4 Microplate (12 x 8 wells)", "number":"AB100735-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Platelet Factor 4 (PF4) also known as CXCL4 is a member of the chemokine (C-X-C motif) ligand family. This small protein has a molecular weight of approximately 7.8 kDa. PF4 is mainly expressed in megakaryocytes and stored in the alpha granules of platelets. During platelet activation PF4 releases into the bloodstream where it interacts with other proteins and cell types. Researchers measure PF4 levels with methods such as PF4 ELISA to study its expression and functions under various physiological and pathological conditions.

Biological function summary

PF4 plays an important role in modulating the immune response and coagulation processes. PF4 is not part of a stable protein complex but can interact dynamically with heparin on the surface of endothelial cells and glycosaminoglycans. It inhibits the action of heparin promoting clot formation and angiogenesis. PF4 also attracts and activates different types of leukocytes and can regulate inflammatory processes influencing how the immune system responds to injury.

Pathways

PF4 significantly interacts with both the coagulation and inflammation pathways. In the coagulation pathway PF4 acts to neutralize heparin-like molecules thereby enhancing thrombosis. It works closely with proteins such as thrombin and fibrinogen contributing to the blood clot cascade. Within the inflammatory pathway PF4's chemokine activity helps direct leukocytes to sites of tissue damage or infection linking it to cytokines and other chemokines that mediate immune responses.

Researchers associate PF4 with conditions such as thrombosis and autoimmune pathologies. In thrombosis the overexpression of PF4 can lead to excessive clot formation pointing to its interaction with proteins like thrombin. PF4 also plays a role in heparin-induced thrombocytopenia (HIT) a disorder where the immune system mistakenly produces antibodies against heparin-PF4 complexes further indicating its direct involvement with immune and coagulation pathways.

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Product protocols

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Target data

Chemokine released during platelet aggregation that plays a role in different biological processes including hematopoiesis, cell proliferation, differentiation, and activation. Acts via different functional receptors including CCR1, CXCR3A or CXCR3B. Upon interaction with CXCR3A receptor, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling. Neutralizes the anticoagulant effect of heparin by binding more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Plays a role in the inhibition of hematopoiesis and in the maintenance of hematopoietic stem cell (HSC) quiescence. Chemotactic for neutrophils and monocytes via CCR1. Inhibits endothelial cell proliferation. In cooperation with toll-like receptor 8/TLR8, induces chromatin remodeling and activates inflammatory gene expression via the TBK1-IRF5 axis. In addition, induces myofibroblast differentiation and collagen synthesis in different precursor cells, including endothelial cells, by stimulating endothelial-to-mesenchymal transition. Interacts with thrombomodulin/THBD to enhance the activation of protein C and thus potentiates its anticoagulant activity.

See full target information Pf4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 5: PubMed32641582

2020

Monocyte upregulation of podoplanin during early sepsis induces complement inhibitor release to protect liver function.

Applications

Unspecified application

Species

Unspecified reactive species

Zhanli Xie,Bojing Shao,Christopher Hoover,Michael McDaniel,Jianhua Song,Miao Jiang,Zhenni Ma,Fei Yang,Jingjing Han,Xia Bai,Changgeng Ruan,Lijun Xia

Blood 122:2723-31 PubMed24004668

2013

β1 integrin-mediated signals are required for platelet granule secretion and hemostasis in mouse.

Applications

Unspecified application

Species

Mouse

Tobias Petzold,Raphael Ruppert,Dharmendra Pandey,Verena Barocke,Hannelore Meyer,Michael Lorenz,Lin Zhang,Wolfgang Siess,Steffen Massberg,Markus Moser

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse PF4 ELISA Kit (CXCL4)

Sandwich ELISA - Mouse PF4 ELISA Kit (CXCL4) (AB100735)

ELISA - Mouse PF4 ELISA Kit (CXCL4) (AB100735)

Sandwich ELISA - Mouse PF4 ELISA Kit (CXCL4) (AB100735)

ELISA - Mouse PF4 ELISA Kit (CXCL4) (AB100735)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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