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AB209879

Mouse Pin1 ELISA Kit

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Mouse Pin1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Pin1 with a sensitivity of 11.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Peptidyl-prolyl cis-trans isomerase Pin1, PPIase Pin1, Pin1

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Sandwich ELISA - Mouse Pin1 ELISA Kit (AB209879)
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Supplier Data

Sandwich ELISA - Mouse Pin1 ELISA Kit (AB209879)

Example of mouse Pin1 standard curve in 1X Cell Extraction Buffer PTR.

The Pin1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Pin1 ELISA Kit (AB209879)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Pin1 ELISA Kit (AB209879)

Representative image of Mouse Pin1 ELISA Kit (ab209879)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Mouse Pin1 ELISA Kit Capture Antibody

- 10X Mouse Pin1 Detector Antibody

- 10X Mouse Pin1 ELISA Kit Lyophilised Purified Protein (2 vials)

- Mouse Pin1 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Mouse Pin1 ELISA Kit (AB209879)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Pin1 ELISA Kit (AB209879)

This assay detects human and rat PP2A-alpha.

The concentrations of PP2A-alpha in PC12 and HeLa extract were measured in duplicate, interpolated from the mouse PP2A-alpha standard curves and corrected for sample dilution. Undiluted samples are PC12 extract based on a 25 μg/mL extract load and HeLa extract based on a 12.5 μg/mL extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PP2A-alpha concentration was determined to be 1,763 pg/mL in PC12 extract and 763 pg/mL in HeLa extract.

Sandwich ELISA - Mouse Pin1 ELISA Kit (AB209879)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Pin1 ELISA Kit (AB209879)

Interpolated concentrations of native Pin1 in mouse NIH-3T3 extract based on a 35 μg/mL extract load and mouse brain extract based on a 50 μg/mL extract load.

The concentrations of Pin1 were measured in duplicate and interpolated from the Pin1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pin1 concentration was determined to be 906 pg/mL in NIH-3T3 extract and 1,803 pg/mL in mouse brain extract.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 11.2 pg/mL

Range

46.9 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Pin1 ELISA Kit (ab209879) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Pin1 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Pin1 with 11.2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Pin1 is a peptidyl-prolyl cis/trans isomerase, isomerizing specific phosphorylated Ser/Thr-Pro motifs present in some proteins. Pin1 is involved in the regulation of mitosis, and it can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability, and cell transformation. Upregulation of Pin1 is associated with certain cancers, while downregulation of Pin1 has been implicated in Alzheimer’s disease.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "94 - 111 %", "average": "= 105" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab209879-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB209879-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB209879-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB209879-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB209879-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB209879-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB209879-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Pin1 Lyophilized Recombinant Protein", "number":"AB209879-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB209879-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Pin1 Capture Antibody", "number":"AB209879-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB209879-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Pin1 Detector Antibody", "number":"AB209879-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB209879-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pin1 also known as Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 is a small isomerase enzyme with a molecular weight of approximately 18 kDa. This protein is unique because of its specificity for phosphorylated serine/threonine-proline bonds. Pin1 catalyzes the cis-trans isomerization of these bonds with high efficiency. Expression of the Pin1 protein occurs widely across various tissues such as the brain heart and liver. It predominantly functions in the cytoplasm and nucleus where it modulates the activity of its target proteins by inducing conformational changes.
Biological function summary

Pin1 is essential in regulating cell cycle progression and cellular signaling. It influences a variety of cellular processes including cell proliferation transcriptional regulation and apoptosis. By altering the conformation of specific phosphorylated proteins Pin1 ensures the precise control of cell cycle checkpoints impacting the stability of many proteins such as cyclin D1 and p53. Despite not being part of a large protein complex Pin1 interacts with numerous other proteins thereby coordinating complex regulatory networks essential for maintaining normal cell function.

Pathways

Pin1 plays an important role in both the Wnt signaling pathway and the MAPK signaling pathway. In the Wnt pathway Pin1 maintains stability and the accumulation of β-catenin which affects transcription of target genes critical for cell proliferation. Within the MAPK pathway Pin1 regulates the activity of proteins like c-Jun and ERK1/2 which are vital for transmitting extracellular signals to cellular responses. Through these pathways Pin1 helps modulate responses to external stimuli and maintains cellular homeostasis by adjusting protein function dynamically in response to changes in phosphorylation status.

Changes in Pin1 activity have been linked to cancer and Alzheimer's disease. In cancer increased Pin1 expression accelerates the degradation of tumor suppressor proteins like p53 contributing to uncontrolled cell proliferation and reduced apoptosis. In Alzheimer's disease Pin1 dysfunction leads to the accumulation of phosphorylated tau protein which forms neurofibrillary tangles and disrupts normal neuronal function. Through these connections Pin1 represents a potential therapeutic target for developing treatments aimed at restoring balance in these pathways and alleviating disease symptoms.
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Product protocols

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Target data

Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs (PubMed : 29686383). By inducing conformational changes in a subset of phosphorylated proteins, acts as a molecular switch in multiple cellular processes. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK. Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation (By similarity). Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner (PubMed : 17828269). Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation : degradation of FBXW7 leads to subsequent stabilization of JUN (By similarity). May facilitate the ubiquitination and proteasomal degradation of RBBP8/CtIP through CUL3/KLHL15 E3 ubiquitin-protein ligase complex, hence favors DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR) (By similarity). Upon IL33-induced lung inflammation, catalyzes cis-trans isomerization of phosphorylated IRAK3/IRAK-M, inducing IRAK3 stabilization, nuclear translocation and expression of pro-inflammatory genes in dendritic cells (PubMed : 29686383).
See full target information Pin1
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websiteProtocolBooklet
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