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Mouse Plasminogen Total ELISA Kit (PLG) is a Quantitative ELISA kit for the measurement of Mouse Plasminogen Total (PLG) in Mouse in Urine, Plasma, Tissue Extracts, Cell culture media, Serum samples.
Alternative names=Plasminogen, Plg
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture media, Serum
- Assay type
Quantitative
- Reactive species
Mouse
- Range
1 - 500 ng/mL
- Sensitivity
= 0.38 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
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Mouse Plasminogen Total ELISA Kit (PLG) is a Quantitative ELISA kit for the measurement of Mouse Plasminogen Total (PLG) in Mouse in Urine, Plasma, Tissue Extracts, Cell culture media, Serum samples.
Alternative names=Plasminogen, Plg
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture media, Serum
- Assay type
Quantitative
- Reactive species
Mouse
- Range
1 - 500 ng/mL
- Assay Platform
Microplate
- Sensitivity
= 0.38 ng/mL
- General recovery
99.25%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Spike | n 20 | mean 8.4 ng/mL | SD 0.575 | C.V. 6.85 |
Sample Spike | n 20 | mean 13.2 ng/mL | SD 0.884 | C.V. 6.72 |
Sample Spike | n 20 | mean 150 ng/mL | SD 11.4 | C.V. 7.61 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Spike | n 10 | mean 14 ng/mL | SD 1.33 | C.V. 9.51 |
Sample Spike | n 10 | mean 7.93 ng/mL | SD 0.736 | C.V. 9.29 |
Sample Spike | n 10 | mean 149 ng/mL | SD 8.76 | C.V. 5.87 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Spike | Average % = 99.5 | Range 96 - 103 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Abcam's Plasminogen Total (PLG) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of total plasminogen in mouse plasma, serum, urine, cell culture media, or tissue extracts.
Mouse plasminogen will bind to the capture antibody coated on the microtiter plate. Plasminogen, plasmin, and plasmin in complex with antiplasmin will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-mouse plasminogen primary antibody binds to the plasminogen. Excess antibody is washed away and bound polyclonal antibody is then bound by the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of total plasminogen in the sample.
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