Mouse Plasminogen Total ELISA Kit (PLG) is a sandwich ELISA designed to quantify total mouse plasminogen with a sensitivity of 0.38 ng/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 1 - 500 ng/mL
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Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Plasma, Tissue Extracts, Cell culture media, Serum
- Assay type
- Quantitative
- Reactive species
- Mouse
- Range
- 1 - 500 ng/mL
- Sensitivity
- = 0.38 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells (By similarity). Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.
Alternative names
Plasminogen, Plg
What's included?
Recommended products
Mouse Plasminogen Total ELISA Kit (PLG) is a sandwich ELISA designed to quantify total mouse plasminogen with a sensitivity of 0.38 ng/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 1 - 500 ng/mL
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Plasma, Tissue Extracts, Cell culture media, Serum
- Assay type
- Quantitative
- Reactive species
- Mouse
- Range
- 1 - 500 ng/mL
- Assay Platform
- Microplate
- Sensitivity
- = 0.38 ng/mL
- General recovery
- 99.25%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Spike | n 20 | mean 8.4 ng/mL | SD 0.575 | C.V. 6.85 |
Sample Spike | n 20 | mean 13.2 ng/mL | SD 0.884 | C.V. 6.72 |
Sample Spike | n 20 | mean 150 ng/mL | SD 11.4 | C.V. 7.61 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Spike | n 10 | mean 14 ng/mL | SD 1.33 | C.V. 9.51 |
Sample Spike | n 10 | mean 7.93 ng/mL | SD 0.736 | C.V. 9.29 |
Sample Spike | n 10 | mean 149 ng/mL | SD 8.76 | C.V. 5.87 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Spike | Average % = 99.5 | Range 96 - 103 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Abcam's Plasminogen Total (PLG) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of total plasminogen in mouse plasma, serum, urine, cell culture media, or tissue extracts.
Mouse plasminogen will bind to the capture antibody coated on the microtiter plate. Plasminogen, plasmin, and plasmin in complex with antiplasmin will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-mouse plasminogen primary antibody binds to the plasminogen. Excess antibody is washed away and bound polyclonal antibody is then bound by the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of total plasminogen in the sample.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Plasminogen also known as PLG is an important protein with a molecular weight of approximately 92 kDa. Plasminogen is a zymogen which means it is an inactive precursor that requires activation to function. It is produced primarily in the liver and circulates in blood plasma. Its expression occurs mainly in the liver but you can detect it in various tissues including the kidney. Plasminogen binds to tissue surfaces and undergoes transformation into plasmin an active enzyme that plays significant roles in many biological processes.
- Biological function summary
Plasminogen plays a central role in fibrinolysis which is the breakdown of fibrin clots. This process is essential in wound healing and maintaining normal blood flow. Plasminogen once activated to plasmin acts by degrading fibrin a major component of blood clots. The protein forms part of the fibrinolytic system along with other components like tissue plasminogen activator (tPA). Together they ensure that any clots forming within blood vessels are regulated and dissolved as necessary preventing blockages.
- Pathways
Plasminogen is deeply involved in the coagulation and fibrinolytic systems. The conversion of plasminogen to plasmin is a critical step in these pathways requiring activators such as urokinase. Plasminogen has interactions with proteins like alpha-2-antiplasmin and thrombin which help modulate its activity. These interactions ensure a careful balance is maintained between clot formation and dissolution which is vital for normal bodily function.
- Associated diseases and disorders
Plasminogen deficiency is linked with conditions like ligneous conjunctivitis a rare disorder characterized by membranous lesions mainly in the eyes. Reduced plasminogen activity can lead to excessive clot formation contributing to thrombosis risk. Plasminogen's interaction with plasmin and its deficiency can also associate with angioedema a condition that causes sudden swelling in the deeper layers of skin and tissues. Understanding the pathways and the proteins involved in plasminogen's function presents therapeutic potential in managing these disorders.
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2 product images
Sandwich ELISA - Mouse Plasminogen Total ELISA Kit (PLG) (ab198511)
Full range standard curve using ab198511 Plasminogen Total (PLG) Mouse ELISA Kit.
Sandwich ELISA - Mouse Plasminogen Total ELISA Kit (PLG) (ab198511)
Linear range standard curve using ab198511 Plasminogen Total (PLG) Mouse ELISA Kit.
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