Mouse PLGF-2 ELISA Kit (PGF) is a Sandwich (quantitative) ELISA kit for the measurement of Mouse PLGF-2 (PGF) in Mouse in Plasma, Cell culture supernatant, Serum samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 1.64 - 400 pg/mL
- Sensitivity
- < 1 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed:11593004). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low density lipoprotein particles and limits vascular damage (By similarity).
Alternative names
Myeloperoxidase, MPO, Mpo
What's included?
Recommended products
Mouse PLGF-2 ELISA Kit (PGF) is a Sandwich (quantitative) ELISA kit for the measurement of Mouse PLGF-2 (PGF) in Mouse in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 1.64 - 400 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 1 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 110.3 | Range 104 - 116 % |
Sample type Serum | Average % = 95.3 | Range 87 - 103 % |
Sample type Plasma | Average % = 91.02 | Range 74 - 107 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's PLGF-2 (PGF) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse PLGF-2 (PGF) in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse PLGF-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and PLGF-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse PLGF-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PLGF-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The standard is affinity purified.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Mouse PLGF-2 ELISA Kit (PGF) (ab155459)
Representative standard curve using ab155459 - Assay Diluent C.
Sandwich ELISA - Mouse PLGF-2 ELISA Kit (PGF) (ab155459)
Representative standard curve using ab155459 - Assay Diluent B.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com