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AB155459

Mouse PLGF-2 ELISA Kit (PGF)

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Mouse PLGF-2 ELISA Kit (PGF) is a Sandwich (quantitative) ELISA for the measurement of Mouse PLGF-2 (PGF) in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Myeloperoxidase, MPO, Mpo

2 Images
Sandwich ELISA - Mouse PLGF-2 ELISA Kit (PGF) (AB155459)
  • sELISA

Unknown

Sandwich ELISA - Mouse PLGF-2 ELISA Kit (PGF) (AB155459)

Representative standard curve using ab155459 - Assay Diluent B.

Sandwich ELISA - Mouse PLGF-2 ELISA Kit (PGF) (AB155459)
  • sELISA

Unknown

Sandwich ELISA - Mouse PLGF-2 ELISA Kit (PGF) (AB155459)

Representative standard curve using ab155459 - Assay Diluent C.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 1 pg/mL

Range

1.64 - 400 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's PLGF-2 (PGF) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse PLGF-2 (PGF) in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse PLGF-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and PLGF-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse PLGF-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PLGF-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

The standard is affinity purified.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "104 - 116 %", "average": "= 110.3" }, { "sample": "Serum", "range": "87 - 103 %", "average": "= 95.3" }, { "sample": "Plasma", "range": "74 - 107 %", "average": "= 91.02" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155459-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155459-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155459-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"PLGF-2 Microplate (12 x 8 wells)", "number":"AB155459-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155459-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse PLGF-2 Standard (lyophilized)", "number":"AB155459-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155459-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB155459-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse PLGF-2", "number":"AB155459-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB155459-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 11593004). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low density lipoprotein particles and limits vascular damage (By similarity).
See full target information Mpo
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websiteProtocolBooklet
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Product promise

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