Mouse PLGF ELISA Kit

Product details

Mouse PLGF ELISA Kit (ab197748) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PLGF protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PLGF with 0.526 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Placental growth factor (PLGF) is an angiogenic factor that is variably glycosylated and secreted. The PLGF protein is a 55-60 kDa disulfide linked homodimer and it belongs to the small family of PDGF/VEGF-related angiogenic growth factors, all of which contain 8 conserved cysteines. Due to alternative splicing, there are three human PLGF mRNAs encoding human PLGF-1, -2, and -3 isoform precursors. There is a unique 21 aa insert at the C-terminus of the PLGF-2 isoform and this isoform is the heparin binding form of PLGF. In mouse tissues there is currently only one identified PLGF mRNA form encoding the equivalent of human PLGF-2. Mouse PLGF is a 158 aa polypeptide that contains an 18 aa signal sequence and a 140 aa mature segment. Human and rat PLGF share 66% and 91% aa sequence identity, respectively, to mouse PLGF.Homodimeric mouse PLGF binds the tyrosine kinase receptor VEGF R1/Flt-1. This receptor is a 180 kDa type I transmembrane glycoprotein with high affinity for PLGF. In addition to forming homodimers, PLGF also forms heterodimers with VEGF-A isoforms, forming bioactive molecules. When heterodimerized with VEGF, PLGF will activate VEGF R2.A limited number of functions have been directly attributed to PLGF. It is known to induce monocyte activation and to provide a stimulus for monocyte migration. PLGF also upregulates VEGF production in PBMCs and induces tissue factor secretion in both monocytes and HUVECs. Furthermore, PLGF is also reported to serve as a growth and chemotactic factor for endothelial cells. Finally, PLGF is known to be expressed by trophoblasts and may protect against growth factor withdrawal-induced trophoblast apoptosis.The standard is affinity purified.