Mouse PLTP ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse PLTP in Mouse in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.
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Key facts
- Detection method
Colorimetric
- Sample types
Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
0.78 - 50 ng/mL
- Assay time
3h 30m
- Sensitivity
< 20 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
Phospholipid transfer protein, Lipid transfer protein II, Pltp
What's included?
Recommended products
Mouse PLTP ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse PLTP in Mouse in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.
Alternative names
Phospholipid transfer protein, Lipid transfer protein II, Pltp
Key facts
- Detection method
Colorimetric
- Sample types
Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
0.78 - 50 ng/mL
- Assay time
3h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
< 20 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample 3 | n 16 | mean 22.189 ng/mL | SD 1.02 | C.V. = 4.6 |
Sample Sample 1 | n 16 | mean 1.418 ng/mL | SD 0.087 | C.V. = 6.2 |
Sample Sample 2 | n 16 | mean 4.309 ng/mL | SD 0.284 | C.V. = 6.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample 2 | n 24 | mean 4.212 ng/mL | SD 0.32 | C.V. = 7.6 |
Sample Sample 3 | n 24 | mean 21.284 ng/mL | SD 1.213 | C.V. = 5.7 |
Sample Sample 1 | n 24 | mean 1.296 ng/mL | SD 0.099 | C.V. = 7.7 |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
The Mouse PLTP Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213883) is designed for the quantitative measurement of Mouse PLTP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for PLTP has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: E18- A493) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLTP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PLTP amount of sample captured in plate.
Phospholipid transfer protein (PLTP), also known as lipid transfer protein II is a protein that in humans is encoded by the PLTP gene. This gene is mapped to 20q13.12. The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene.
Supplementary info
- Activity summary
PLTP also known as Phospholipid Transfer Protein is an important component in human lipid metabolism. This protein functions by transferring phospholipids between different types of lipoproteins in plasma. PLTP possesses a molecular mass of about 56 kDa. Its expression occurs mainly in the liver and adipose tissue with additional presence in the brain and lungs. These locales of expression facilitate its key roles in lipid processing and lipoprotein metabolism.
- Biological function summary
PLTP plays a significant role in remodeling high-density lipoproteins (HDL) and regulating lipoprotein particles. PLTP exists as a part of a larger complex involved in these processes interacting closely with other proteins in the lipoprotein maturation and transformation. This activity of PLTP significantly affects the levels and sizes of HDL particles in circulation thereby influencing cholesterol homeostasis within the body.
- Pathways
PLTP acts within the reverse cholesterol transport pathway important for the removal of excess cholesterol from peripheral tissues. The protein works alongside Apolipoprotein A-I (ApoA-I) and Lecithin-Cholesterol Acyltransferase (LCAT) to promote cholesterol efflux and HDL maturation. Another key pathway involving PLTP is the lipid metabolism pathway where it influences lipid levels and impacts the function of other proteins such as Apolipoprotein E (ApoE).
- Associated diseases and disorders
PLTP has associations with atherosclerosis and metabolic syndrome. Variations in PLTP activity or expression can lead to altered HDL levels increasing the risk for heart disease and related cardiovascular conditions. In atherosclerosis PLTP's relationship with ApoA-I becomes especially relevant as this interaction influences lipid transport dynamics and plaque formation. Additionally in metabolic syndrome abnormal PLTP function may contribute to dyslipidemia a common factor in metabolic disorders.
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Sandwich ELISA - Mouse PLTP ELISA Kit (ab213883)
Mouse PLTP ELISA Kit (ab213883) Standard Curve.
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