AB208984

Mouse PON2 ELISA Kit

Recombinant
SimpleStep
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Mouse PON2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse PON2 with a sensitivity of 41 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Serum paraoxonase/arylesterase 2, PON 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2, A-esterase 2, Pon2

4 Images

Sandwich ELISA - Mouse PON2 ELISA Kit (AB208984)

sELISA

Supplier Data

Sandwich ELISA - Mouse PON2 ELISA Kit (AB208984)

Example of mouse PON2 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse PON2 ELISA Kit (AB208984)

Rat liver extract was diluted 2-fold in 1x Cell Extraction Buffer PTR and assayed for cross-reactivity.

Interpolated values from the mouse PON2 protein standard are graphed. Undiluted Rat liver extract is at 12.5 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse PON2 ELISA Kit (AB208984)

Interpolated concentrations of native mouse PON2 in mouse liver, spleen, and NIH/3T3 cell extract samples.

Undiluted liver, spleen, and NIH/3T3 extract is at 31,62.5, and 25 μg/mL extract, respectively The concentrations of mouse PON2 were measured in duplicate and interpolated from the mouse PON2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse PON2 ELISA Kit (AB208984)

Representative image of Mouse PON2 ELISA Kit (ab208984)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Mouse PON2 ELISA Kit Capture Antibody

- 10X Mouse PON2 Detector Antibody

- 10X Mouse PON2 ELISA Kit Lyophilised Purified Protein (2 vials)

- Mouse PON2 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4B

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 41 pg/mL

Range

78.13 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse PON2 ELISA Kit (ab208984) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PON2 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PON2 with 41 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Serum Paraoxonase (PON2) is a 39-45 kDa, calcium binding, membrane-bound protein, ubiquitously expressed in mouse tissue. PON2 exhibits hydrolytic activity against acylhomoserine lactones, which are important bacterial quorum-sensing mediators, which might suggest a role in the defense response to pathogenic bacteria. Mutations of this gene have been suggested to be associated to vascular disease and with a number of quantitative phenotypes related to diabetes.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Mouse Spleen", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" }, { "reproducibilityType": "Intra", "sample": "Mouse Spleen", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]

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Recovery

[ { "sample": "Cell culture extracts", "range": "93 - 99 %", "average": "= 95" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab208984-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB208984-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB208984-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB208984-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB208984-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB208984-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB208984-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse PON2 Capture Antibody", "number":"AB208984-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse PON2 Lyophilized Recombinant Protein", "number":"AB208984-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BC", "number":"AB208984-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse PON2 Detector Antibody", "number":"AB208984-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB208984-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB208984-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters.

See full target information Pon2

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse PON2 ELISA Kit

Sandwich ELISA - Mouse PON2 ELISA Kit (AB208984)

Sandwich ELISA - Mouse PON2 ELISA Kit (AB208984)

Sandwich ELISA - Mouse PON2 ELISA Kit (AB208984)

Sandwich ELISA - Mouse PON2 ELISA Kit (AB208984)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Complementary Products

Primary Antibodies

Assay Kits

Assay Kits

Assay Kits

Product promise