Mouse PON3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse PON3 with a sensitivity of 5.36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.23 - 15 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 5.36 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Has low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents (By similarity).
Alternative names
Serum paraoxonase/lactonase 3, Pon3
What's included?
Recommended products
Mouse PON3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse PON3 with a sensitivity of 5.36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.23 - 15 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 5.36 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 5 | mean - | SD - | C.V. 4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 3 | mean - | SD - | C.V. 4.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 97 | Range 93 - 101 % |
Sample type Serum | Average % = 110 | Range 108 - 111 % |
Sample type Cell culture extracts | Average % = 102 | Range 101 - 104 % |
Sample type Tissue Extracts | Average % = 112 | Range 108 - 115 % |
Sample type Citrate plasma | Average % = 110 | Range 107 - 112 % |
Sample type EDTA Plasma | Average % = 109 | Range 107 - 111 % |
Sample type Fetal Bovine Serum | Average % = 99 | Range 98 - 99 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse PON3 ELISA Kit (ab211653) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PON3 protein in cell culture extracts, cell culture supernatant, plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PON3 with 5.36 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Serum paraoxonase/lactonase 3 (PON3) is a calcium binding 354 amino acid protein. PON3 possesses hydrolytic catalytic activity against aromatic and 5- or 6- member ring lactones. PON3 is a member of the paraoxonase family consisting of PON1 and PON, which are involved in modulating oxidative stress and possess anti-atherogenic properties. PON3 is a secreted protein that associates with high-density lipoprotein (HDL).
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Sandwich ELISA - Mouse PON3 ELISA Kit (ab211653)
Example of mouse PON3 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse PON3 ELISA Kit (ab211653)
Interpolated concentrations of spiked recombinant PON3 in pooled samples.
Interpolated concentrations of spiked recombinant PON3 in pooled normal mouse serum, plasma (citrate), and cell culture media (RPMI 1640 + 10% fetal bovine serum) samples, and native PON3 in plasma (EDTA) samples. The concentrations of PON3 were measured in duplicate, interpolated from the PON3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 20% and cell culture media 32.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Mouse PON3 ELISA Kit (ab211653)
Interpolated concentrations of native PON3 in RAW 264.7 cell extract and spiked recombinant PON3 in mouse liver extract based on a 1,000 μg/mL extract load.
The concentrations of PON3 were measured in duplicate and interpolated from the PON3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Mouse PON3 ELISA Kit (ab211653)
Native levels of mouse PON3 as measured by SimpleStep ELISA (mean +/- SD).
Dilutions of pooled normal mouse serum and plasma within the linear range were measured in duplicate, their background-subtracted values interpolated off of the mouse PON3 standard curve, and corrected for dilution back to neat. The mean PON3 concentration was determined to be 6.7 ng/mL in serum, 4.5 ng/mL in plasma (citrate), 17.8 ng/mL in plasma (EDTA), and 6 ng/mL in RAW 264.7 cell culture supernatant.
Sandwich ELISA - Mouse PON3 ELISA Kit (ab211653)
Native levels of mouse PON3 as measured by SimpleStep ELISA (mean +/- SD).
Dilutions of cell and tissue extracts within the linear range were measured in duplicate, their background-subtracted values interpolated off of the mouse PON3 standard curve, and corrected for dilution back to a 1000 μg extract load. The mean PON3 concentration was determined to be 71.9 ng/mg in liver extract and 669 ng/mg in RAW 264.7 cell extract.
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