JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB209878

Mouse PP2A-alpha ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse PP2A-alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse PP2A-alpha with a sensitivity of 66.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, Ppp2ca

3 Images
Sandwich ELISA - Mouse PP2A-alpha ELISA Kit (AB209878)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PP2A-alpha ELISA Kit (AB209878)

Interpolated concentrations of native PP2A in mouse NIH-3T3 extract based on a 12 μg/mL extract load and mouse skeletal muscle extract based on a 20 μg/mL extract load.

The concentrations of PP2A-alpha were measured in duplicate and interpolated from the PP2A-alpha standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PP2A-alpha concentration was determined to be 4,273 pg/mL in NIH-3T3 extract and 2,719 pg/mL in mouse skeletal muscle extract.

Sandwich ELISA - Mouse PP2A-alpha ELISA Kit (AB209878)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PP2A-alpha ELISA Kit (AB209878)

Example of mouse PP2A-alpha standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse PP2A-alpha ELISA Kit (AB209878)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PP2A-alpha ELISA Kit (AB209878)

This assay detects human and rat PP2A-alpha.

The concentrations of PP2A-alpha in PC12 and HeLa extract were measured in duplicate, interpolated from the mouse PP2A-alpha standard curves and corrected for sample dilution. Undiluted samples are PC12 and HeLa extract based on a 10 μg/mL extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PP2A-alpha concentration was determined to be 4,956 pg/mL in PC12 extract and 4,395 pg/mL in HeLa extract.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Tissue Homogenate

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 66.2 pg/mL

Range

85.9 - 5500 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Mouse PP2A-alpha ELISA Kit (ab209878) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PP2A-alpha protein in tissue extracts, tissue homogenate, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PP2A-alpha with 66.2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

PP2A-alpha is a highly conserved heterotrimeric phosphatase for microtubule-associated proteins. Additionally, PP2A-alpha targets include proteins in oncogenic signalling cascades, including AKT, MEK and Raf.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.3" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" } ]
style
left-column

Recovery

[ { "sample": "Cell culture extracts", "range": "98 - 106 %", "average": "= 102" }, { "sample": "Tissue Extracts", "range": "92 - 100 %", "average": "= 97" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab209878-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB209878-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB209878-CMP12", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB209878-CMP14", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse PP2A-alpha Capture Antibody", "number":"AB209878-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse PP2A-alpha Lyophilized Recombinant Protein", "number":"AB209878-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB209878-CMP13", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB209878-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB209878-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB209878-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB209878-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB209878-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse PP2A-alpha Detector Antibody", "number":"AB209878-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Catalytic subunit of protein phosphatase 2A (PP2A), a serine/threonine phosphatase involved in the regulation of a wide variety of enzymes, signal transduction pathways, and cellular events. PP2A is the major phosphatase for microtubule-associated proteins (MAPs) (By similarity). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase (By similarity). Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (PubMed : 18084284). Can dephosphorylate p53/TP53 (By similarity). Activates RAF1 by dephosphorylating it at 'Ser-259' (By similarity). Mediates dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint (By similarity). Mediates dephosphorylation of MYC; promoting its ubiquitin-mediated proteolysis : interaction with AMBRA1 enhances interaction between PPP2CA and MYC (PubMed : 25438055). Mediates dephosphorylation of FOXO3; promoting its stabilization : interaction with AMBRA1 enhances interaction between PPP2CA and FOXO3 (By similarity). Catalyzes dephosphorylation of the pyrin domain of NLRP3, promoting assembly of the NLRP3 inflammasome (PubMed : 28465465). Together with RACK1 adapter, mediates dephosphorylation of AKT1 at 'Ser-473', preventing AKT1 activation and AKT-mTOR signaling pathway (PubMed : 26974206, PubMed : 33505023). Dephosphorylation of AKT1 is essential for regulatory T-cells (Treg) homeostasis and stability (PubMed : 33505023). Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (By similarity).
See full target information Ppp2ca
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Proteins & Peptides

AB114424

Recombinant Human PP2A-alpha protein (Tagged)

SDS-PAGE - Recombinant Human PP2A-alpha protein (Tagged) (AB114424)

  • 0
  • 0
Proteins & Peptides

AB268879

Recombinant human PPP2R1A + PP2A alpha protein (Active)

Functional Studies - Recombinant human PPP2R1A + PP2A alpha protein (Active) (AB268879)

  • 0
  • 0
Primary Antibodies

AB32141

Anti-PP2A alpha + beta antibody [Y119]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-PP2A alpha + beta antibody [Y119] (AB32141)

  • 4
  • 5
Reagents, Controls & Accessories

AB203359

Pre-coated 384 well Microplate SimpleStep ELISA®

SimpleStep

ELISA - Pre-coated 384 well Microplate SimpleStep ELISA® (AB203359)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com