Mouse PR3 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse PR3 in Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
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- International journal of biological sciences 19:5233-52442023Screening of an FDA-approved compound library identifies apigenin for the treatment of myocardial injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaixia Li,Dong Chen,Xiaoqin Zhang,Mingxian Chen,Yinghao Zhi,Weilu Cui,Shanshan Li,Fan Xu,Ying Tan,Hao Zhou,Xing Chang,Hengwen ChenPubMed 37928261
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 195.31 - 12500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 51.21 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration. May play a role in neutrophil transendothelial migration, probably when associated with CD177.
Alternative names
Myeloblastin, Proteinase 3, PR-3, Prtn3
What's included?
Recommended products
Mouse PR3 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse PR3 in Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 195.31 - 12500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 51.21 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 3.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 97 | Range 96 - 102 % |
Sample type Serum | Average % = 102 | Range 94 - 114 % |
Sample type EDTA Plasma | Average % = 99 | Range 81 - 108 % |
Sample type Tissue Extracts | Average % = 97 | Range 97 - 97 % |
Sample type Heparin Plasma | Average % = 99 | Range 95 - 107 % |
Sample type Citrate plasma | Average % = 90 | Range 100 - 107 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse PR3 ELISA kit (ab277720) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse PR3 protein in mouse serum, plasma, cell and tissue culture supernatant, and cell and tissue culture extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PR3 with 51.21 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mouse PR3 is a polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro). PR3 causes emphysema when administered by tracheal insufflation to hamsters. Mouse PR3 shares 93.5%, 66.9%, 70.7%, and 66.3% sequence homology with rat, human, cow, and monkey, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Proteinase 3 (PR3) also known as PRTN3 is a serine protease with a mass of approximately 29 kDa. It belongs to the human neutrophil elastase (HNE) family and is mainly expressed in neutrophils. Within these immune cells PR3 localizes to azurophilic granules where it participates in early immune responses. The proteolytic activity of PR3 involves cleaving peptide bonds in target proteins contributing to the processing and degradation of various proteins important for immune function.
- Biological function summary
Proteinase 3 serves multiple roles including modulating inflammation and immune responses. PR3 functions as part of a complex with azurophilic granules aiding in microbial defense mechanisms. In addition the protease regulates cytokine activation and deactivation which in turn influences cell signaling related to inflammation. Its enzymatic action ensures that PR3 can manage inflammatory processes by breaking down extracellular matrix components thereby allowing white blood cells to access infection sites.
- Pathways
Proteinase 3 plays an important role within the serine protease and inflammatory pathways. It interacts with other proteins such as neutrophil elastase with which it shares a pathway to modulate immune responses. PR3 also partners with cathepsin G contributing to processes such as pathogen clearance and tissue remodeling providing a coordinated response during infection and injury. These interactions show PR3's involvement in regulating the balance between inflammation and tissue repair.
- Associated diseases and disorders
PR3 is notably associated with Wegener's granulomatosis also known as granulomatosis with polyangiitis. This autoimmune condition results in systemic vasculitis where anti-proteinase 3 antibodies notably characterize the disease. Additionally PR3 plays a part in chronic obstructive pulmonary disease (COPD) where its proteolytic activity contributes to tissue damage in the lungs. In both conditions PR3 interplays with related proteins like myeloperoxidase further demonstrating its importance in processes leading to these disorders.
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4 product images
Sandwich ELISA - Mouse PR3 ELISA Kit (ab277720)
Example of mouse PR3 standard curve in Sample Diluent NSThe PR3 standard curve was prepared as described in Section 10.
Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse PR3 ELISA Kit (ab277720)
Example of mouse PR3 standard curve in 1X Cell Extraction Buffer PTR.
The PR3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse PR3 ELISA Kit (ab277720)
Interpolated concentrations of native PR3 in mouse serum, plasma and cell culture supernatant samples.
The concentrations of PR3 were measured in duplicates, interpolated from the PR3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (citrate) 2.5%, plasma (EDTA) 2.5%, plasma (heparin) 2.5% and lung supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PR3 concentration was determined to be 210.96 ng/mL in serum, 487.99 pg/mL in plasma (citrate) 156.49 pg/mL in plasma (EDTA), 243.00 pg/mL in plasma (heparin), and 11.72 ng/mL in lung supernatant.
Sandwich ELISA - Mouse PR3 ELISA Kit (ab277720)
Interpolated concentrations of native PR3 in mouse spleen extract based on a 20 μg/mL extract load mouse bone marrow extract based on a 1 μg/mL extract load.
The concentrations of PR3 were measured in duplicate and interpolated from the PR3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PR3 concentration was determined to be 8.38 ng/mL in spleen extract and 7.15 ng/mL in bone marrow extract.
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