AB277720

Mouse PR3 ELISA Kit

Recombinant
SimpleStep
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Mouse PR3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse PR3 with a sensitivity of 51.21 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Myeloblastin, Proteinase 3, PR-3, Prtn3

4 Images

Sandwich ELISA - Mouse PR3 ELISA Kit (AB277720)

sELISA

Supplier Data

Sandwich ELISA - Mouse PR3 ELISA Kit (AB277720)

Example of mouse PR3 standard curve in Sample Diluent NS. The PR3 standard curve was prepared as described in Section 10.

Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse PR3 ELISA Kit (AB277720)

Interpolated concentrations of native PR3 in mouse serum, plasma and cell culture supernatant samples.

The concentrations of PR3 were measured in duplicates, interpolated from the PR3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, plasma (citrate) 2.5%, plasma (EDTA) 2.5%, plasma (heparin) 2.5% and lung supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PR3 concentration was determined to be 210.96 ng/mL in serum, 487.99 pg/mL in plasma (citrate) 156.49 pg/mL in plasma (EDTA), 243.00 pg/mL in plasma (heparin), and 11.72 ng/mL in lung supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse PR3 ELISA Kit (AB277720)

Interpolated concentrations of native PR3 in mouse spleen extract based on a 20 μg/mL extract load mouse bone marrow extract based on a 1 μg/mL extract load.

The concentrations of PR3 were measured in duplicate and interpolated from the PR3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PR3 concentration was determined to be 8.38 ng/mL in spleen extract and 7.15 ng/mL in bone marrow extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse PR3 ELISA Kit (AB277720)

Example of mouse PR3 standard curve in 1X Cell Extraction Buffer PTR.

The PR3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 51.21 pg/mL

Range

195.31 - 12500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse PR3 ELISA kit (ab277720) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse PR3 protein in mouse serum, plasma, cell and tissue culture supernatant, and cell and tissue culture extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PR3 with 51.21 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Mouse PR3 is a polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro). PR3 causes emphysema when administered by tracheal insufflation to hamsters. Mouse PR3 shares 93.5%, 66.9%, 70.7%, and 66.3% sequence homology with rat, human, cow, and monkey, respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "96 - 102 %", "average": "= 97" }, { "sample": "Serum", "range": "94 - 114 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "81 - 108 %", "average": "= 99" }, { "sample": "Tissue Extracts", "range": "97 - 97 %", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "95 - 107 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "100 - 107 %", "average": "= 90" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277720-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB277720-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB277720-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse PR3 Detector Antibody", "number":"AB277720-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB277720-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB277720-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB277720-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB277720-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB277720-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB277720-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse PR3 Lyophilized Recombinant Protein", "number":"AB277720-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse PR3 Capture Antibody", "number":"AB277720-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteinase 3 (PR3) also known as PRTN3 is a serine protease with a mass of approximately 29 kDa. It belongs to the human neutrophil elastase (HNE) family and is mainly expressed in neutrophils. Within these immune cells PR3 localizes to azurophilic granules where it participates in early immune responses. The proteolytic activity of PR3 involves cleaving peptide bonds in target proteins contributing to the processing and degradation of various proteins important for immune function.

Biological function summary

Proteinase 3 serves multiple roles including modulating inflammation and immune responses. PR3 functions as part of a complex with azurophilic granules aiding in microbial defense mechanisms. In addition the protease regulates cytokine activation and deactivation which in turn influences cell signaling related to inflammation. Its enzymatic action ensures that PR3 can manage inflammatory processes by breaking down extracellular matrix components thereby allowing white blood cells to access infection sites.

Pathways

Proteinase 3 plays an important role within the serine protease and inflammatory pathways. It interacts with other proteins such as neutrophil elastase with which it shares a pathway to modulate immune responses. PR3 also partners with cathepsin G contributing to processes such as pathogen clearance and tissue remodeling providing a coordinated response during infection and injury. These interactions show PR3's involvement in regulating the balance between inflammation and tissue repair.

PR3 is notably associated with Wegener's granulomatosis also known as granulomatosis with polyangiitis. This autoimmune condition results in systemic vasculitis where anti-proteinase 3 antibodies notably characterize the disease. Additionally PR3 plays a part in chronic obstructive pulmonary disease (COPD) where its proteolytic activity contributes to tissue damage in the lungs. In both conditions PR3 interplays with related proteins like myeloperoxidase further demonstrating its importance in processes leading to these disorders.

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Product protocols

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Target data

Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration. May play a role in neutrophil transendothelial migration, probably when associated with CD177.

See full target information Myeloblastin

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 19:5233-5244 PubMed37928261

2023

Screening of an FDA-approved compound library identifies apigenin for the treatment of myocardial injury.

Applications

Unspecified application

Species

Unspecified reactive species

Haixia Li,Dong Chen,Xiaoqin Zhang,Mingxian Chen,Yinghao Zhi,Weilu Cui,Shanshan Li,Fan Xu,Ying Tan,Hao Zhou,Xing Chang,Hengwen Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse PR3 ELISA Kit

Sandwich ELISA - Mouse PR3 ELISA Kit (AB277720)

Sandwich ELISA - Mouse PR3 ELISA Kit (AB277720)

Sandwich ELISA - Mouse PR3 ELISA Kit (AB277720)

Sandwich ELISA - Mouse PR3 ELISA Kit (AB277720)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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