AB325450

Mouse Prealbumin ELISA Kit, Chemiluminescent

SimpleStep
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Mouse Prealbumin ELISA Kit, Chemiluminescent is a 55-min single-wash Simplestep Ignite^TM ELISA for the rapid quantification of Mouse Prealbumin with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range :110 - 80000 pg/mL with a sensitivity of 44.08 pg/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent Pending

View Alternative Names

Transthyretin, Prealbumin, Ttr

5 Images

Sandwich ELISA - Mouse Prealbumin ELISA Kit, Chemiluminescent (AB325450)

sELISA

Lab

Sandwich ELISA - Mouse Prealbumin ELISA Kit, Chemiluminescent (AB325450)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

sELISA

Lab

Sandwich ELISA - Mouse Prealbumin ELISA Kit, Chemiluminescent (AB325450)

Interpolated concentration of native Prealbumin was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 50 mouse liver tissue supernatant. The interpolated dilution factor corrected values to neat sample are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Mouse Prealbumin ELISA Kit, Chemiluminescent (AB325450)

Interpolated concentration of native Prealbumin was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 25,000 mouse serum, plasma-EDTA, plasma-Citrate, and Plasma-Heparin. The interpolated dilution factor corrected values to neat sample are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Mouse Prealbumin ELISA Kit, Chemiluminescent (AB325450)

Dynamic range comparison between the Chemiluminescent Mouse Prealbumin SimpleStep ELISA, colorimetric Mouse Prealbumin SimpleStep ELISA (ab282297), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

sELISA

Lab

Sandwich ELISA - Mouse Prealbumin ELISA Kit, Chemiluminescent (AB325450)

Standard curve comparison between the Chemiluminescent Mouse Prealbumin SimpleStep ELISA and colorimetric Mouse Prealbumin SimpleStep ELISA (ab282297) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 44.08 pg/mL

Range

110 - 80000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Prealbumin ELISA Kit, Chemiluminescent (ab325450) is a rapid single-wash sandwich 55-min ELISA to measure Mouse Prealbumin in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Culture Supernatant with a sensitivity of 44.08 pg/ml.

How the assay works

Mouse Prealbumin ELISA Kit, Chemiluminescent (ab325450) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

Please refer to our protocol booklet for more details.

Mouse Prealbumin (ab325450) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature

2. Wash

3. Add prepared ChemiHRP development solution - incubate for 2 min

4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-TTR antibody [EPR20971-130] - BSA and Azide free (Capture) ab283848

- Anti-TTR antibody [EPR20971-140] - BSA and Azide free (Detector) ab285122

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.7" } ]

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Recovery

[ { "sample": "Serum", "range": "109 - 118 %", "average": "= 113" }, { "sample": "Citrate plasma", "range": "102 - 113 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "91 - 96 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "93 - 99 %", "average": "= 95" }, { "sample": "Cell culture supernatant", "range": "100 - 110 %", "average": "= 106" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325450-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Mouse Prealbumin Detector Antibody", "number":"AB325450-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325450-CMP05", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325450-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Prealbumin Lyophilized Recombinant Protein", "number":"AB325450-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Prealbumin Capture Antibody", "number":"AB325450-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325450-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325450-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325450-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB325450-CMP04", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325450-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.

See full target information Ttr

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com