Mouse Pro-Collagen I alpha 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Pro-Collagen I alpha 1 with a sensitivity of 6.7 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Type I collagen is a member of group I collagen (fibrillar forming collagen).
Cola1, Col1a1, Collagen alpha-1(I) chain, Alpha-1 type I collagen
Mouse Pro-Collagen I alpha 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Pro-Collagen I alpha 1 with a sensitivity of 6.7 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations
Sample | n | C.V. |
---|---|---|
Sample Serum | n 8 | C.V. 2.4 |
Sample | n | C.V. |
---|---|---|
Sample Serum | n 3 | C.V. 5.6 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 106 | Range 102 - 109 % |
Sample type Citrate plasma | Average % = 115 | Range 113 - 116 % |
Sample type EDTA Plasma | Average % = 109 | Range 102 - 116 % |
Sample type Heparin Plasma | Average % = 111 | Range 104 - 115 % |
Mouse Pro-Collagen I alpha 1 ELISA Kit ab210579 is a rapid single-wash 90-min ELISA kit to measure Mouse Pro-Collagen I alpha 1 in cell culture extracts, tissue extracts, serum and plasma. This SimpleStep ELISA® sensitivity is 6.7 pg/mL.
How the assay works
Mouse Pro-Collagen I alpha 1 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant mouse Pro-Collagen I alpha 1 protein in serum, plasma, and cell and tissue extract samples only. This kit is not validated for cell culture supernatants.
Mouse Pro-Collagen I alpha 1 ELISA Kit ab210579 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Mouse Pro-Collagen I alpha 1 ELISA kit ab210579
Mouse Pro-Collagen I alpha 1 ELISA kit ab210579 has been used to study the effects of ethanol on osteoclast differentiation (1) and the effects of therapeutic candidates for skeletal disorders (2).
References:
(1)-Marin J J Ronis et al. 2022, PMID: 34755883,
(2)Shinsuke Ohba et al. 2022, PMID: 36475024
Save your precious samples
Our 384-well Mouse Pro-Collagen I alpha 1 ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Mouse Pro-Collagen I alpha 1 Antibody Pair - BSA and Azide free Mouse Pro-Collagen I alpha 1 Antibody Pair - BSA and Azide free ab241818
- Anti-Procollagen Type I antibody [EPR19075-180] - BSA and Azide free (Capture) Anti-Procollagen Type I antibody [EPR19075-180] - BSA and Azide free (Capture) ab242554
- Anti-Procollagen Type I antibody [EPR19075-139] - BSA and Azide free (Detector) Anti-Procollagen Type I antibody [EPR19075-139] - BSA and Azide free (Detector) ab242802
Related and recommended products
Mouse Pro-Collagen I alpha 1 ELISA kit ab210579 is commonly used to study tissue structure and function. Pro-Collagen I alpha 1 is a component of the extracellular matrix (ECM) and provides structural support to bones.
Other ELISA kits to measure the ECM and its role in tissue function include:
- Mouse Fibronectin ELISA kit Mouse Fibronectin ELISA Kit ab210967
- Human Pro-Collagen I alpha 1 ELISA kit Human Pro-Collagen I alpha 1 ELISA Kit ab210966
ELISA kits to measure bone formation and turnover include:
- Mouse Osteocalcin ELISA kit Mouse Osteocalcin ELISA Kit ab285236
- Human Osteocalcin ELISA kit Human Osteocalcin ELISA Kit ab270202
Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a pro-collagen molecule that is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. Specifically, mouse Pro-Collagen I alpha 1 consists of a signal peptide (amino acids (aa) 1-22), a propeptide (aa 23-151), the mature chain (aa 152-1207), and another propeptide (aa 1208 – 1453). The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues.
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Example of mouse Pro-Collagen I alpha 1 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Example of mouse Pro-Collagen I alpha 1 standard curve in Sample Diluent NS.
The Pro-Collagen I alpha 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Example of mouse Pro-Collagen I alpha 1 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Example of mouse Pro-Collagen I alpha 1 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.
The Pro-Collagen I alpha 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of native Pro-Collagen I alpha 1 in mouse serum and plasma samples.
The concentrations of Pro-Collagen I alpha 1 were measured in duplicates, interpolated from the Pro-Collagen I alpha 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:1,200, plasma (citrate) 1:1,200, plasma (EDTA) 1:1,200 and plasma (heparin) 1:1,200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 1,822 ng/mL in serum, 1,128 ng/mL in plasma (citrate), 1,628 ng/mL in plasma (EDTA), and 1,734 ng/mL in plasma (heparin).
Interpolated concentrations of native Pro-Collagen I alpha 1 in mouse NIH 3T3 cell extract based on a 10 μg/mL extract load and mouse skin tissue extract based on a 20 μg/mL extract load.
The concentrations of Pro-Collagen I alpha 1 were measured in duplicate and interpolated from the Pro-Collagen I alpha 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 1,751 pg/mL in NIH 3T3 cell extract and 851 pg/mL in mouse skin tissue extract.
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native mouse Pro-Collagen I alpha 1 was measured in serum, plasma, and cell and tissue extract samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS for serum and plasma samples. Sample dilutions are made in Sample Diluent 1X Cell Extraction Buffer PTR for cell and tissue extract samples.
Assay sensitivity.
The calculated minimal detectable dose (MDD) is determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration. The MDD is dependent on the Sample Diluent buffer used.
Example of mouse Pro-Collagen I alpha 1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Example of mouse Pro-Collagen I alpha 1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:1,200 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Example of mouse Pro-Collagen I alpha 1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 5 µg/mL NIH/3T3 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR
Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:1,200 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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