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Mouse Pro-Collagen I alpha 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Pro-Collagen I alpha 1 with a sensitivity of 6.7 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

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Images

ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579), expandable thumbnail
  • Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579), expandable thumbnail
  • Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579), expandable thumbnail
  • Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579), expandable thumbnail
  • Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579), expandable thumbnail

Publications

Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture extracts, Tissue Extracts, Serum
Assay type
Sandwich (quantitative)
Reactive species
Mouse
Range
31.3 - 2000 pg/mL
Assay time
1h 30m
Sensitivity
= 6.7 pg/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Type I collagen is a member of group I collagen (fibrillar forming collagen).

Alternative names

What's included?

1 x 384 Tests
Components
10X Mouse Pro-Collagen I alpha 1 Capture Antibody
1 x 600 µL
10X Mouse Pro-Collagen I alpha 1 Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
384 well CaptSure™ microplates
1 x 1 Unit
50X Cell Extraction Enhancer Solution (ab193971)
6 x 1 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 50 mL
Antibody Diluent 5BR
1 x 6 mL
Mouse Pro-Collagen I alpha 1 Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 250 mL
Stop Solution
2 x 12 mL
TMB Development Solution
2 x 12 mL

Recommended products

Mouse Pro-Collagen I alpha 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Pro-Collagen I alpha 1 with a sensitivity of 6.7 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture extracts, Tissue Extracts, Serum
Assay type
Sandwich (quantitative)
Reactive species
Mouse
Range
31.3 - 2000 pg/mL
Assay time
1h 30m
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 6.7 pg/mL

Precision

Intra assay

Sample
Serum
n
8
C.V.
2.4

Inter assay

Sample
Serum
n
3
C.V.
5.6

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 106
Range
102 - 109 %
Sample type
Citrate plasma
Average %
= 115
Range
113 - 116 %
Sample type
EDTA Plasma
Average %
= 109
Range
102 - 116 %
Sample type
Heparin Plasma
Average %
= 111
Range
104 - 115 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Mouse Pro-Collagen I alpha 1 ELISA Kit ab210579 is a rapid single-wash 90-min ELISA kit to measure Mouse Pro-Collagen I alpha 1 in cell culture extracts, tissue extracts, serum and plasma. This SimpleStep ELISA® sensitivity is 6.7 pg/mL.

How the assay works

Mouse Pro-Collagen I alpha 1 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and recombinant mouse Pro-Collagen I alpha 1 protein in serum, plasma, and cell and tissue extract samples only. This kit is not validated for cell culture supernatants.

Mouse Pro-Collagen I alpha 1 ELISA Kit ab210579 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Mouse Pro-Collagen I alpha 1 ELISA kit ab210579

Mouse Pro-Collagen I alpha 1 ELISA kit ab210579 has been used to study the effects of ethanol on osteoclast differentiation (1) and the effects of therapeutic candidates for skeletal disorders (2).

References:

(1)-Marin J J Ronis et al. 2022, PMID: 34755883,
(2)Shinsuke Ohba et al. 2022, PMID: 36475024

Save your precious samples

Our 384-well Mouse Pro-Collagen I alpha 1 ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Mouse Pro-Collagen I alpha 1 Antibody Pair - BSA and Azide free Mouse Pro-Collagen I alpha 1 Antibody Pair - BSA and Azide free ab241818
- Anti-Procollagen Type I antibody [EPR19075-180] - BSA and Azide free (Capture) Anti-Procollagen Type I antibody [EPR19075-180] - BSA and Azide free (Capture) ab242554
- Anti-Procollagen Type I antibody [EPR19075-139] - BSA and Azide free (Detector) Anti-Procollagen Type I antibody [EPR19075-139] - BSA and Azide free (Detector) ab242802

Related and recommended products

Mouse Pro-Collagen I alpha 1 ELISA kit ab210579 is commonly used to study tissue structure and function. Pro-Collagen I alpha 1 is a component of the extracellular matrix (ECM) and provides structural support to bones.

Other ELISA kits to measure the ECM and its role in tissue function include:

- Mouse Fibronectin ELISA kit Mouse Fibronectin ELISA Kit ab210967
- Human Pro-Collagen I alpha 1 ELISA kit Human Pro-Collagen I alpha 1 ELISA Kit ab210966

ELISA kits to measure bone formation and turnover include:

- Mouse Osteocalcin ELISA kit Mouse Osteocalcin ELISA Kit ab285236
- Human Osteocalcin ELISA kit Human Osteocalcin ELISA Kit ab270202

Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a pro-collagen molecule that is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. Specifically, mouse Pro-Collagen I alpha 1 consists of a signal peptide (amino acids (aa) 1-22), a propeptide (aa 23-151), the mature chain (aa 152-1207), and another propeptide (aa 1208 – 1453). The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

  • ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579), expandable thumbnail

    ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)

  • Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579), expandable thumbnail

    Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)

    Example of mouse Pro-Collagen I alpha 1 standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579), expandable thumbnail

    Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)

    Example of mouse Pro-Collagen I alpha 1 standard curve in Sample Diluent NS.

    The Pro-Collagen I alpha 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579), expandable thumbnail

    Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)

    Example of mouse Pro-Collagen I alpha 1 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579), expandable thumbnail

    Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)

    Example of mouse Pro-Collagen I alpha 1 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.

    The Pro-Collagen I alpha 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579), expandable thumbnail

    Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)

    Interpolated concentrations of native Pro-Collagen I alpha 1 in mouse serum and plasma samples.

    The concentrations of Pro-Collagen I alpha 1 were measured in duplicates, interpolated from the Pro-Collagen I alpha 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:1,200, plasma (citrate) 1:1,200, plasma (EDTA) 1:1,200 and plasma (heparin) 1:1,200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 1,822 ng/mL in serum, 1,128 ng/mL in plasma (citrate), 1,628 ng/mL in plasma (EDTA), and 1,734 ng/mL in plasma (heparin).

  • Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579), expandable thumbnail

    Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)

    Interpolated concentrations of native Pro-Collagen I alpha 1 in mouse NIH 3T3 cell extract based on a 10 μg/mL extract load and mouse skin tissue extract based on a 20 μg/mL extract load.

    The concentrations of Pro-Collagen I alpha 1 were measured in duplicate and interpolated from the Pro-Collagen I alpha 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 1,751 pg/mL in NIH 3T3 cell extract and 851 pg/mL in mouse skin tissue extract.

  • Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579), expandable thumbnail

    Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)

    Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

    Native mouse Pro-Collagen I alpha 1 was measured in serum, plasma, and cell and tissue extract samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS for serum and plasma samples. Sample dilutions are made in Sample Diluent 1X Cell Extraction Buffer PTR for cell and tissue extract samples.

  • Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579), expandable thumbnail

    Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)

    Assay sensitivity.

    The calculated minimal detectable dose (MDD) is determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration. The MDD is dependent on the Sample Diluent buffer used.

  • Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579), expandable thumbnail

    Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)

    Example of mouse Pro-Collagen I alpha 1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579), expandable thumbnail

    Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)

    Example of mouse Pro-Collagen I alpha 1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579), expandable thumbnail

    Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)

    Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:1,200 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

  • Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579), expandable thumbnail

    Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)

    Example of mouse Pro-Collagen I alpha 1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579), expandable thumbnail

    Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)

    Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 5 µg/mL NIH/3T3 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR

  • Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579), expandable thumbnail

    Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)

    Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:1,200 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

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