AB210579

Mouse Pro-Collagen I alpha 1 ELISA Kit

Name: Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579) | Abcam
Brand: Abcam Limited
SKU: AB210579
Price: 835 USD
Availability: InStock
Rating: 5 (1 reviews)

Recombinant
SimpleStep
What is this?

(1 Review)

(21 Publications)

Mouse Pro-Collagen I alpha 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Pro-Collagen I alpha 1 with a sensitivity of 6.7 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

Cola1, Col1a1, Collagen alpha-1(I) chain, Alpha-1 type I collagen

15 Images

ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

ELISA

Supplier Data

ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

sELISA

Lab

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Interpolated concentrations of mouse Pro-Collagen I alpha 1 in serum in 96-well vs. 384-well plates

Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 1,200 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Interpolated concentrations of mouse Pro-Collagen I alpha 1 in NIH/3T3 cell extract in 96-well vs. 384-well plates

Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 5 µg/mL NIH/3T3 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR

sELISA

Lab

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Example of mouse Pro-Collagen I alpha 1 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Example of mouse Pro-Collagen I alpha 1 standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Example of mouse Pro-Collagen I alpha 1 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Example of mouse Pro-Collagen I alpha 1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Interpolated concentrations of native Pro-Collagen I alpha 1 in mouse serum and plasma samples.

The concentrations of Pro-Collagen I alpha 1 were measured in duplicates, interpolated from the Pro-Collagen I alpha 1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 1,200, plasma (citrate) 1 : 1,200, plasma (EDTA) 1 : 1,200 and plasma (heparin) 1 : 1,200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 1,822 ng/mL in serum, 1,128 ng/mL in plasma (citrate), 1,628 ng/mL in plasma (EDTA), and 1,734 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native mouse Pro-Collagen I alpha 1 was measured in serum, plasma, and cell and tissue extract samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS for serum and plasma samples. Sample dilutions are made in Sample Diluent 1X Cell Extraction Buffer PTR for cell and tissue extract samples.

sELISA

Supplier Data

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Example of mouse Pro-Collagen I alpha 1 standard curve in Sample Diluent NS.

The Pro-Collagen I alpha 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Interpolated concentrations of native Pro-Collagen I alpha 1 in mouse NIH 3T3 cell extract based on a 10 μg/mL extract load and mouse skin tissue extract based on a 20 μg/mL extract load.

The concentrations of Pro-Collagen I alpha 1 were measured in duplicate and interpolated from the Pro-Collagen I alpha 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 1,751 pg/mL in NIH 3T3 cell extract and 851 pg/mL in mouse skin tissue extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Assay sensitivity.

The calculated minimal detectable dose (MDD) is determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration. The MDD is dependent on the Sample Diluent buffer used.

sELISA

Lab

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

sELISA

Supplier Data

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Example of mouse Pro-Collagen I alpha 1 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.

The Pro-Collagen I alpha 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Example of mouse Pro-Collagen I alpha 1 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture extracts, Tissue Extracts, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6.7 pg/mL

Range

31.3 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Pro-Collagen I alpha 1 ELISA Kit ab210579 is a rapid single-wash 90-min ELISA kit to measure Mouse Pro-Collagen I alpha 1 in cell culture extracts, tissue extracts, serum and plasma. This SimpleStep ELISA® sensitivity is 6.7 pg/mL.

How the assay works

Mouse Pro-Collagen I alpha 1 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and recombinant mouse Pro-Collagen I alpha 1 protein in serum, plasma, and cell and tissue extract samples only. This kit is not validated for cell culture supernatants.

Mouse Pro-Collagen I alpha 1 ELISA Kit ab210579 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Mouse Pro-Collagen I alpha 1 ELISA kit ab210579

Mouse Pro-Collagen I alpha 1 ELISA kit ab210579 has been used to study the effects of ethanol on osteoclast differentiation ⁽¹⁾ and the effects of therapeutic candidates for skeletal disorders ⁽²⁾.

References:

⁽¹⁾-Marin J J Ronis et al. 2022, PMID: 34755883,
⁽²⁾Shinsuke Ohba et al. 2022, PMID: 36475024

Save your precious samples

Our 384-well Mouse Pro-Collagen I alpha 1 ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Mouse Pro-Collagen I alpha 1 Antibody Pair - BSA and Azide free ab241818
- Anti-Procollagen Type I antibody [EPR19075-180] - BSA and Azide free (Capture) ab242554
- Anti-Procollagen Type I antibody [EPR19075-139] - BSA and Azide free (Detector) ab242802

Related and recommended products

Mouse Pro-Collagen I alpha 1 ELISA kit ab210579 is commonly used to study tissue structure and function. Pro-Collagen I alpha 1 is a component of the extracellular matrix (ECM) and provides structural support to bones.

Other ELISA kits to measure the ECM and its role in tissue function include:

- Mouse Fibronectin ELISA kit ab210967
- Human Pro-Collagen I alpha 1 ELISA kit ab210966

ELISA kits to measure bone formation and turnover include:

- Mouse Osteocalcin ELISA kit ab285236
- Human Osteocalcin ELISA kit ab270202

Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a pro-collagen molecule that is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. Specifically, mouse Pro-Collagen I alpha 1 consists of a signal peptide (amino acids (aa) 1-22), a propeptide (aa 23-151), the mature chain (aa 152-1207), and another propeptide (aa 1208 – 1453). The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]

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Recovery

[ { "sample": "Serum", "range": "102 - 109 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "113 - 116 %", "average": "= 115" }, { "sample": "EDTA Plasma", "range": "102 - 116 %", "average": "= 109" }, { "sample": "Heparin Plasma", "range": "104 - 115 %", "average": "= 111" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.

Biological function summary

Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.

Pathways

Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.

Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.

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Product protocols

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Target data

Type I collagen is a member of group I collagen (fibrillar forming collagen).

See full target information Col1a1

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 41:33 PubMed39825191

2025

Deciphering SPP1-related macrophage signaling in the pathogenesis of intervertebral disc degeneration.

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Unspecified application

Species

Unspecified reactive species

Xiao-Jun Yu,Peng Zou,Tian-Qi Li,Xiao-Fan Bai,Shan-Xi Wang,Jian-Bin Guan,Yuan-Ting Zhao,Meng-Wei Li,Xiaodong Wang,Ying-Guang Wang,Ding-Jun Hao

Physiological reports 12:e16136 PubMed39501714

2024

Evaluating the antifibrotic potential of naringenin, asiatic acid, and icariin using murine and human precision-cut liver slices.

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Unspecified application

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Ke Luo,Yana Geng,Dorenda Oosterhuis,Vincent E de Meijer,Peter Olinga

Clinical and translational medicine 14:e1800 PubMed39305055

2024

IFIT3 mediates TBK1 phosphorylation to promote activation of pDCs and exacerbate systemic sclerosis in mice.

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Unspecified application

Species

Unspecified reactive species

Xiangyang Huang,Yi Liu,Xia Rong,Yiheng Zhao,Dan Feng,Jun Wang,Wanhong Xing

Experimental & molecular medicine 56:2052-2064 PubMed39261656

2024

IKKε-deficient macrophages impede cardiac repair after myocardial infarction by enhancing the macrophage-myofibroblast transition.

Applications

Unspecified application

Species

Unspecified reactive species

Hyang Hee Cho,Siyeon Rhee,Dong Im Cho,Ju Hee Jun,HyoJung Heo,Su Han Cho,Dohyup Kim,Mingqiang Wang,Bo Gyeong Kang,Soo Ji Yoo,Meeyoung Cho,Soo Yeon Lim,Jae Yeong Cho,In Seok Jeong,Yong Sook Kim,Youngkeun Ahn

Nature communications 15:6540 PubMed39095402

2024

Lipid droplet-associated hydrolase mobilizes stores of liver X receptor sterol ligands and protects against atherosclerosis.

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Unspecified application

Species

Unspecified reactive species

Young-Hwa Goo,Janeesh Plakkal Ayyappan,Francis D Cheeran,Sushant Bangru,Pradip K Saha,Paula Baar,Sabine Schulz,Todd A Lydic,Bernhard Spengler,Andreas H Wagner,Auinash Kalsotra,Vijay K Yechoor,Antoni Paul

JCI insight 9: PubMed38780544

2024

Methionine as a regulator of bone remodeling with fasting.

Applications

Unspecified application

Species

Unspecified reactive species

Tânia Amorim,Naveen Gv Kumar,Natalie L David,William Dion,Trishya Pagadala,Nandini K Doshi,Bokai Zhu,Andrey Parkhitko,Matthew L Steinhauser,Pouneh K Fazeli

Basic research in cardiology 119:243-260 PubMed38329499

2024

Mineralocorticoid receptor promotes cardiac macrophage inflammaging.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Fraccarollo,Robert Geffers,Paolo Galuppo,Johann Bauersachs

Theranostics 14:608-621 PubMed38169629

2024

Inactivation of the NLRP3 inflammasome mediates exosome-based prevention of atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Sandrine Parent,Ramana Vaka,Jennifer St Amant,Saad Kahn,Sophie Van Remortel,Christina Bi,David Courtman,Duncan John Stewart,Darryl Raymond Davis

Journal of molecular and cellular cardiology plus 6:100056 PubMed38143961

2023

Inhibition of miR-199a-3p in a murine hypertrophic cardiomyopathy (HCM) model attenuates fibrotic remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Irina Zalivina,Temo Barwari,Xiaoke Yin,Sarah R Langley,Javier Barallobre-Barreiro,Hiroko Wakimoto,Anna Zampetaki,Manuel Mayr,Metin Avkiran,Seda Eminaga

Regenerative therapy 21:584-595 PubMed36475024

2022

Orally administrable peptides derived from egg yolk promote skeletal repair and ameliorate degenerative skeletal disorders in mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshiaki Kitaura,Utano Nakamura,Chihiro Awada,Motonori Yamaguchi,Mujo Kim,Yuki Ikeda,Yuki Matsuo,Takeshi Moriishi,Takashi Sawase,Ung-Il Chung,Hironori Hojo,Shinsuke Ohba

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Pro-Collagen I alpha 1 ELISA Kit

ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (AB210579)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

Product promise