Mouse Pro-Collagen I alpha 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Pro-Collagen I alpha 1 with a sensitivity of 6.7 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations
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Publications
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- Journal of molecular and cellular cardiology plus 6:1000562023Inhibition of miR-199a-3p in a murine hypertrophic cardiomyopathy (HCM) model attenuates fibrotic remodeling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrina Zalivina,Temo Barwari,Xiaoke Yin,Sarah R Langley,Javier Barallobre-Barreiro,Hiroko Wakimoto,Anna Zampetaki,Manuel Mayr,Metin Avkiran,Seda EminagaPubMed 38143961
- Regenerative therapy 21:584-5952022Orally administrable peptides derived from egg yolk promote skeletal repair and ameliorate degenerative skeletal disorders in mouse models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshiaki Kitaura,Utano Nakamura,Chihiro Awada,Motonori Yamaguchi,Mujo Kim,Yuki Ikeda,Yuki Matsuo,Takeshi Moriishi,Takashi Sawase,Ung-Il Chung,Hironori Hojo,Shinsuke OhbaPubMed 36475024
- Clinical and translational medicine 12:e9822022Loss of MEN1 leads to renal fibrosis and decreases HGF-Adamts5 pathway activity via an epigenetic mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBangming Jin,Jiamei Zhu,Yuxia Zhou,Li Liang,Yunqiao Yang,Lifen Xu,Tuo Zhang,Po Li,Ting Pan,Bing Guo,Tengxiang Chen,Haiyang LiPubMed 35968938
- Nutrients 13:2021The Effects of Butyrate on Induced Metabolic-Associated Fatty Liver Disease in Precision-Cut Liver Slices.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGrietje H Prins,Melany Rios-Morales,Albert Gerding,Dirk-Jan Reijngoud,Peter Olinga,Barbara M BakkerPubMed 34959755
- Toxicological sciences : an official journal of the Society of Toxicology 185:232-2452021Binge Ethanol Exposure in Mice Represses Expression of Genes Involved in Osteoblast Function and Induces Expression of Genes Involved in Osteoclast Differentiation Independently of Endogenous Catalase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandra Denys,Kim B Pedersen,James Watt,Allison R Norman,Michelle L Osborn,Jin-Ran Chen,Cole Maimone,Shana Littleton,Vasilis Vasiliou,Martin J J RonisPubMed 34755883
- International journal of molecular sciences 22:2021The Protective Role of Prolyl Oligopeptidase (POP) Inhibition in Kidney Injury Induced by Renal Ischemia-Reperfusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiovanna Casili,Alessio Ardizzone,Rossella Basilotta,Marika Lanza,Alessia Filippone,Irene Paterniti,Emanuela Esposito,Michela CampoloPubMed 34769337
- Cell reports 35:1090152021Cytoplasmic polyadenylation by TENT5A is required for proper bone formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlga Gewartowska,Goretti Aranaz-Novaliches,Paweł S Krawczyk,Seweryn Mroczek,Monika Kusio-Kobiałka,Bartosz Tarkowski,Frantisek Spoutil,Oldrich Benada,Olga Kofroňová,Piotr Szwedziak,Dominik Cysewski,Jakub Gruchota,Marcin Szpila,Aleksander Chlebowski,Radislav Sedlacek,Jan Prochazka,Andrzej DziembowskiPubMed 33882302
- Journal of cellular physiology 235:2722-27372019Autophagy inhibition via Becn1 downregulation improves the mesenchymal stem cells antifibrotic potential in experimental liver fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHang Yu Wang,Can Li,Wei Hua Liu,Feng Mei Deng,Yan Ma,Li Na Guo,De Hua Kong,Kang An Hu,Qin Liu,Jiang Wu,Jing Sun,Yi Lun LiuPubMed 31508820
- American journal of physiology. Lung cellular and molecular physiology 316:L348-L3572018Molecular characterization of a precision-cut rat lung slice model for the evaluation of antifibrotic drugs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinqiang Huang,Li Li,Ron Ammar,Yan Zhang,Yihe Wang,Kandasamy Ravi,John Thompson,Gabor JaraiPubMed 30489156
- American journal of physiology. Gastrointestinal and liver physiology 316:G15-G242018Molecular characterization of a precision-cut rat liver slice model for the evaluation of antifibrotic compounds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinqiang Huang,Hong Cai,Ron Ammar,Yan Zhang,Yihe Wang,Kandasamy Ravi,John Thompson,Gabor JaraiPubMed 30406699
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Tissue Extracts, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 31.3 - 2000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 6.7 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Alternative names
Cola1, Col1a1, Collagen alpha-1(I) chain, Alpha-1 type I collagen
What's included?
Recommended products
Mouse Pro-Collagen I alpha 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Pro-Collagen I alpha 1 with a sensitivity of 6.7 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Tissue Extracts, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 31.3 - 2000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 6.7 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 2.4 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 5.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 106 | Range 102 - 109 % |
Sample type Citrate plasma | Average % = 115 | Range 113 - 116 % |
Sample type EDTA Plasma | Average % = 109 | Range 102 - 116 % |
Sample type Heparin Plasma | Average % = 111 | Range 104 - 115 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse Pro-Collagen I alpha 1 ELISA Kit ab210579 is a rapid single-wash 90-min ELISA kit to measure Mouse Pro-Collagen I alpha 1 in cell culture extracts, tissue extracts, serum and plasma. This SimpleStep ELISA® sensitivity is 6.7 pg/mL.
How the assay works
Mouse Pro-Collagen I alpha 1 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant mouse Pro-Collagen I alpha 1 protein in serum, plasma, and cell and tissue extract samples only. This kit is not validated for cell culture supernatants.
Mouse Pro-Collagen I alpha 1 ELISA Kit ab210579 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Mouse Pro-Collagen I alpha 1 ELISA kit ab210579
Mouse Pro-Collagen I alpha 1 ELISA kit ab210579 has been used to study the effects of ethanol on osteoclast differentiation (1) and the effects of therapeutic candidates for skeletal disorders (2).
References:
(1)-Marin J J Ronis et al. 2022, PMID: 34755883,
(2)Shinsuke Ohba et al. 2022, PMID: 36475024
Save your precious samples
Our 384-well Mouse Pro-Collagen I alpha 1 ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Mouse Pro-Collagen I alpha 1 Antibody Pair - BSA and Azide free Mouse Pro-Collagen I alpha 1 Antibody Pair - BSA and Azide free ab241818
- Anti-Procollagen Type I antibody [EPR19075-180] - BSA and Azide free (Capture) Anti-Procollagen Type I antibody [EPR19075-180] - BSA and Azide free (Capture) ab242554
- Anti-Procollagen Type I antibody [EPR19075-139] - BSA and Azide free (Detector) Anti-Procollagen Type I antibody [EPR19075-139] - BSA and Azide free (Detector) ab242802
Related and recommended products
Mouse Pro-Collagen I alpha 1 ELISA kit ab210579 is commonly used to study tissue structure and function. Pro-Collagen I alpha 1 is a component of the extracellular matrix (ECM) and provides structural support to bones.
Other ELISA kits to measure the ECM and its role in tissue function include:
- Mouse Fibronectin ELISA kit Mouse Fibronectin ELISA Kit ab210967
- Human Pro-Collagen I alpha 1 ELISA kit Human Pro-Collagen I alpha 1 ELISA Kit ab210966
ELISA kits to measure bone formation and turnover include:
- Mouse Osteocalcin ELISA kit Mouse Osteocalcin ELISA Kit ab285236
- Human Osteocalcin ELISA kit Human Osteocalcin ELISA Kit ab270202
Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a pro-collagen molecule that is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. Specifically, mouse Pro-Collagen I alpha 1 consists of a signal peptide (amino acids (aa) 1-22), a propeptide (aa 23-151), the mature chain (aa 152-1207), and another propeptide (aa 1208 – 1453). The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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15 product images
ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
Example of mouse Pro-Collagen I alpha 1 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
Example of mouse Pro-Collagen I alpha 1 standard curve in Sample Diluent NS.
The Pro-Collagen I alpha 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
Example of mouse Pro-Collagen I alpha 1 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
Example of mouse Pro-Collagen I alpha 1 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.
The Pro-Collagen I alpha 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
Interpolated concentrations of native Pro-Collagen I alpha 1 in mouse serum and plasma samples.
The concentrations of Pro-Collagen I alpha 1 were measured in duplicates, interpolated from the Pro-Collagen I alpha 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:1,200, plasma (citrate) 1:1,200, plasma (EDTA) 1:1,200 and plasma (heparin) 1:1,200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 1,822 ng/mL in serum, 1,128 ng/mL in plasma (citrate), 1,628 ng/mL in plasma (EDTA), and 1,734 ng/mL in plasma (heparin).
Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
Interpolated concentrations of native Pro-Collagen I alpha 1 in mouse NIH 3T3 cell extract based on a 10 μg/mL extract load and mouse skin tissue extract based on a 20 μg/mL extract load.
The concentrations of Pro-Collagen I alpha 1 were measured in duplicate and interpolated from the Pro-Collagen I alpha 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 1,751 pg/mL in NIH 3T3 cell extract and 851 pg/mL in mouse skin tissue extract.
Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native mouse Pro-Collagen I alpha 1 was measured in serum, plasma, and cell and tissue extract samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS for serum and plasma samples. Sample dilutions are made in Sample Diluent 1X Cell Extraction Buffer PTR for cell and tissue extract samples.
Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
Assay sensitivity.
The calculated minimal detectable dose (MDD) is determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration. The MDD is dependent on the Sample Diluent buffer used.
Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
Example of mouse Pro-Collagen I alpha 1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
Example of mouse Pro-Collagen I alpha 1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:1,200 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
Example of mouse Pro-Collagen I alpha 1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 5 µg/mL NIH/3T3 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR
Sandwich ELISA - Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:1,200 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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