Mouse Procollagen III N-terminal propeptide (PIIINP) ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Procollagen III N-terminal propeptide (PIIINP) with a sensitivity of 11.21 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 11.21 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
Alternative names
Collagen alpha-1(III) chain, Col3a1
What's included?
Recommended products
Mouse Procollagen III N-terminal propeptide (PIIINP) ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Procollagen III N-terminal propeptide (PIIINP) with a sensitivity of 11.21 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 11.21 pg/mL
- General recovery
- 95%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 3.8 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 4.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 95 | Range 93 - 96 % |
Sample type Citrate plasma | Average % = 94 | Range 78 - 105 % |
Sample type EDTA Plasma | Average % = 98 | Range 96 - 101 % |
Sample type Heparin Plasma | Average % = 97 | Range 92 - 101 % |
Sample type Cell culture media | Average % = 92 | Range 83 - 98 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse Procollagen III N-terminal propeptide (PIIINP) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Procollagen III N-terminal propeptide (PIIINP) protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media. Quantitate Mouse Procollagen III N-terminal propeptide (PIIINP) with 11.21 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Procollagen III N-terminal propeptide known as PIIINP serves as an important marker in collagen synthesis processes. PIIINP results from the cleavage of procollagen III and functions as a byproduct of the maturation of collagen molecules. It possesses a molecular weight of approximately 42 kDa. This target is initially expressed within fibroblast cells found in connective tissues and then secreted into the bloodstream. Given its role in collagen synthesis it is frequently measured to assess the turnover and formation of collagen in tissues.
- Biological function summary
This propeptide plays a significant role in the formation of the extracellular matrix. PIIINP is not part of a protein complex but accumulates during the conversion of procollagen into mature collagen fibers which later support tissue structure. Its presence is essential for proper collagen deposition and tissue remodeling. Through its involvement in collagen synthesis PIIINP helps maintain the structural integrity of connective tissues across various organs.
- Pathways
Its role reflects the broader functions of collagen metabolism and tissue remodeling pathways. PIIINP marks the processing pathway of procollagen to collagen which is important for the formation of the extracellular matrix. This pathway sometimes intersects with pathways involving other collagens such as collagen I which is another critical component of connective tissues. Together PIIINP and collagen I contribute to the proper maintenance of the extracellular matrix and the mechanical properties of tissues.
- Associated diseases and disorders
Elevated levels of PIIINP often relate to fibrotic conditions and can serve as a biomarker for diseases like liver fibrosis and systemic sclerosis. In liver fibrosis excessive collagen deposition and remodeling occur affecting liver function. In systemic sclerosis abnormal collagen synthesis and deposition in the skin cause thickening and hardening. PIIINP connection to these conditions often involves collagen-binding proteins which regulate collagen stability and deposition.
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4 product images
Sandwich ELISA - Mouse Procollagen III N-terminal propeptide (PIIINP) ELISA Kit (ab320800)
Interpolated concentrations of mouse Procollagen III N-terminal propeptide in serum in 96-well vs. 384-well plates. Interpolated concentration of native Procollagen III N-terminal propeptide was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 5% serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Mouse Procollagen III N-terminal propeptide (PIIINP) ELISA Kit (ab320800)
Example of mouse Procollagen III N-terminal propeptide standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Example of mouse Procollagen III N-terminal propeptide standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Procollagen III N-terminal propeptide (PIIINP) ELISA Kit (ab320800)
Interpolated concentrations of mouse Procollagen III N-terminal propeptide in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Interpolated concentration of native Procollagen III N-terminal propeptide was measured in duplicate at different sample concentrations. Undiluted samples are 5% serum, 10% plasma (citrate), 5% plasma (EDTA), and 5% plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Mouse Procollagen III N-terminal propeptide (PIIINP) ELISA Kit (ab320800)
Example of mouse Procollagen III N-terminal propeptide standard curve in Sample Diluent NS. Example of mouse Procollagen III N-terminal propeptide standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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