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AB213473

Mouse Progranulin ELISA Kit

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Mouse Progranulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Progranulin with a sensitivity of 7.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Progranulin, PGRN, Acrogranin, Epithelin/granulin precursor, Glycoprotein of 88 Kda, PC cell-derived growth factor, Proepithelin, GP88, Glycoprotein 88, PCDGF, PEPI, Grn

11 Images
ELISA - Mouse Progranulin ELISA Kit (AB213473)
  • ELISA

Supplier Data

ELISA - Mouse Progranulin ELISA Kit (AB213473)
Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)

Example of mouse Progranulin standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)

Interpolated concentrations of native Progranulin in mouse serum and plasma samples.

The concentrations of Progranulin were measured in duplicates, interpolated from the Progranulin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 250, plasma (citrate) 1 : 250, plasma (EDTA) 1 : 250, and plasma (heparin) 1 : 250. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Progranulin concentration was determined to be 1,610.5 ng/mL in serum, 1,149.5 ng/mL in plasma (citrate), 1,253.7 ng/mL in plasma (EDTA) and 1,710.8 ng/mL in plasma (heparin).

Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)

Interpolated concentrations of native Progranulin in mouse J774A.1 cell extract based on a 12.5 μg/mL extract load.

The concentrations of Progranulin were measured in duplicate and interpolated from the Progranulin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Progranulin concentration in J774A.1 cell extract was determined to be 2.9 ng/mL.

Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)

Interpolated concentrations of native Progranulin in mouse J774A.1 and RAW264.7 cultured media samples and spiked Progranulin in RPMI culture media.

The concentrations of Progranulin were measured in duplicates, interpolated from the Progranulin standard curves and corrected for sample dilution. Undiluted samples are as follows : J774A.1 cultured media 1 : 400, RAW264.7 cultured media 6.25%, and RPMI culture media 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Progranulin concentration was determined to be 197.4 ng/mL in J774A.1 cultured media, 38.4 ng/mL in RAW264.7 cultured media, and 82.6 ng/mL in RPMI culture media. J774A.1 samples were cultured in high glucose DMEM culture media with kanamycin and 10% fetal bovine serum for 72 hours and then stimulated with 1.5% PHA plus 10 ng/mL PMA for 72 hours. RAW264.7 samples were cultured in high glucose DMEM culture media with kanamycin and L‑Glutamine for 72 hours and then stimulated with 5 μg/mL of LPS for 48 hours.

Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)

Example of mouse Progranulin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)

Interpolated concentrations of native Progranulin in mouse J774A.1 cultured media samples.

All samples were diluted 1 : 400 and the concentrations of Progranulin were measured in duplicates, interpolated from the Progranulin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Progranulin concentration was determined to be 34.8 ng/mL in unstimulated J774A.1 cultured media and 206.9 ng/mL in stimulated J774A.1 cultured media. J774A.1 samples were cultured in high glucose DMEM culture media with kanamycin and 10% fetal bovine serum for 72 hours (unstimulated samples) and then stimulated with 1.5% PHA plus 10 ng/mL PMA for 72 hours.

Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)
  • sELISA

Lab

Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)

Interpolated concentration of native Progranulin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 6.25% stimulated RAW264.7 supernatant (1 µg/mL LPS for 48 hrs).  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)
  • sELISA

Lab

Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)

Example of mouse Progranulin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)
  • sELISA

Lab

Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)

Interpolated concentration of native Progranulin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 12.5 µg/mL J774A.1 cell extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)
  • sELISA

Lab

Sandwich ELISA - Mouse Progranulin ELISA Kit (AB213473)

Example of mouse Progranulin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Sample types

Cell culture extracts, Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 7.8 pg/mL

Range

93.73 - 6000 pg/mL

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Complementary Products

Assay Kits

AB219524

Mouse Progranulin Matched Antibody Pair Kit

Recombinant

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Primary Antibodies

AB187070

Anti-Progranulin antibody [EPR18539-59]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Progranulin antibody [EPR18539-59] (AB187070)

  • 0
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Progranulin ELISA Kit ab213473 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Progranulin in cell culture extracts, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 7.8 pg/mL.

How the assay works

Mouse Progranulin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Progranulin ELISA Kit ab213473 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Mouse Progranulin ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Granulin antibody [EPR18539-39] - BSA and Azide free (Capture) ab242862
- Anti-Granulin antibody [EPR18539-58] - BSA and Azide free (Detector) ab242862

Mouse Progranulin belongs to the granulin family and is 572 amino acids (aa) in length. Additionally, mouse Progranulin is cleaved into individual granulin peptides (Granulin-1 through Granulin-7). Granulins have cytokine-like activity and may also play a role in inflammation, wound repair, and tissue remodelling. They also act as an autocrine growth factor for PC cells. Human and rat Progranulin have 75% and 87% sequence homology with mouse Progranulin, respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "102 - 109 %", "average": "= 107" }, { "sample": "Cell culture media", "range": "93 - 95 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "110 - 119 %", "average": "= 115" }, { "sample": "EDTA Plasma", "range": "114 - 118 %", "average": "= 115" }, { "sample": "Heparin Plasma", "range": "107 - 118 %", "average": "= 111" }, { "sample": "Serum", "range": "115 - 118 %", "average": "= 116" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Granulin also known as progranulin or GRN is a protein with a molecular mass of approximately 68 kDa. This protein is encoded by the GRN gene and is involved in various cellular processes. Granulin is expressed widely within the body particularly in epithelial cells immune cells and neurons. Researchers have developed methods like GRN ELISA and progranulin ELISA to quantitatively measure the levels of granulin in biological samples aiding in further understanding of its role in health and disease.
Biological function summary

Granulin functions as a growth factor involved in tissue repair cell proliferation and inflammation. It does not function as part of a larger protein complex but acts individually in exerting its effects on cells. Granulin influences various aspects of cellular architecture and signaling pathways making it important for maintaining cellular homeostasis. Additionally the granulin protein participates in regulating networks that involve cytokines and growth factors impacting both the innate and adaptive immune responses.

Pathways

Granulin plays significant roles in the MAPK and NF-kB signaling pathways. These pathways critically manage cellular reactions to stress inflammation and other external stimuli. Granulin modulates these processes often interacting with proteins like TNF receptors and interleukins to affect the downstream signaling events. Interactions between granulin and other signaling molecules allow it to integrate into broader networks that oversee various biological responses.

Significant links exist between granulin mutations and frontotemporal dementia (FTD) and Alzheimer's disease. Mutations in the GRN gene often lead to reduced granulin levels contributing to neurodegenerative conditions. These mutations affect the protein's normal function promoting pathological mechanisms associated with neuronal deterioration. Granulin interacts with proteins such as TDP-43 in neurodegenerative diseases where alterations might result in protein aggregation and neurotoxicity. Understanding these connections can lead to potential therapeutic interventions targeting granulin-related pathways.
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Product protocols

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Target data

Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation (PubMed : 12524533, PubMed : 20026663, PubMed : 23041626, PubMed : 27789271, PubMed : 28073925, PubMed : 28453791, PubMed : 28541286, PubMed : 8496151). Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes (PubMed : 27789271, PubMed : 28453791, PubMed : 28541286). Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB (PubMed : 28073925). In addition, functions as a wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures (PubMed : 12524533). Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases (PubMed : 8496151). Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity (PubMed : 20026663, PubMed : 23041626).. Granulin-3. Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8.. Granulin-7. Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity.
See full target information Grn
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Brain : a journal of neurology 140:3215-3232 PubMed29096020

2017

Elevated progranulin contributes to synaptic and learning deficit due to loss of fragile X mental retardation protein.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Zhang,Yu-Jiao Li,Yanyan Guo,Kai-Yin Zheng,Qi Yang,Le Yang,Xin-Shang Wang,Qian Song,Tao Chen,Min Zhuo,Ming-Gao Zhao
View all publications
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Product promise

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