JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB100736

Mouse Prolactin ELISA Kit

5

(1 Review)

|

(19 Publications)

Mouse Prolactin ELISA Kit is a sandwich ELISA designed to quantify Mouse Prolactin with a sensitivity of 30 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 27.43 - 20000 pg/mL
- Cited in over 20 publications

View Alternative Names

Prolactin, PRL, Prl

3 Images
Sandwich ELISA - Mouse Prolactin ELISA Kit (AB100736)
  • sELISA

Unknown

Sandwich ELISA - Mouse Prolactin ELISA Kit (AB100736)

Representative standard curve using ab100736

Sandwich ELISA - Mouse Prolactin ELISA Kit (AB100736)
  • sELISA

Lab

Sandwich ELISA - Mouse Prolactin ELISA Kit (AB100736)

Prolactin measured in mouse biological fluids showing quantity (pg) per mL of tested sample. Samples diluted 10-270 fold.

Sandwich ELISA - Mouse Prolactin ELISA Kit (AB100736)
  • sELISA

Unknown

Sandwich ELISA - Mouse Prolactin ELISA Kit (AB100736)

Representative standard curve using ab100736

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 30 pg/mL

Range

27.43 - 20000 pg/mL

Assay Platform

Microplate

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Mouse Prolactin ELISA Kit ab100736 is a sandwich ELISA to measure Mouse Prolactin in serum, plasma, cell culture supernatant, urine with a sensitivity of 30 pg/ml.

How the assay works

This assay employs an antibody specific for mouse Prolactin coated on a 96-well plate. Standards and samples are pipetted into the wells and Prolactin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Prolactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Prolactin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Prolactin ELISA Kit ab100736 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "68 - 87 %", "average": "= 76.85" }, { "sample": "Serum", "range": "73 - 94 %", "average": "= 82.47" }, { "sample": "Plasma", "range": "67 - 85 %", "average": "= 76.78" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100736-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100736-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100736-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100736-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100736-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB100736-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100736-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse Prolactin Standard (lyophilized)", "number":"AB100736-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100736-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse Prolactin", "number":"AB100736-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Prolactin Microplate (12 x 8 wells)", "number":"AB100736-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prolactin also known as PRL is a protein hormone with a molecular mass of approximately 23 kDa. It is primarily produced in the anterior pituitary gland but is also found in other tissues such as the placenta mammary glands and immune cells. Mechanically Prolactin functions as a peptide hormone and exerts its effects by binding to the Prolactin receptor a member of the cytokine receptor superfamily. This binding activates the Jak-STAT signaling pathway which is important for mediating the hormone's effects in target cells.
Biological function summary

The functions of this hormone extend beyond its well-known role in lactation. It helps stimulate milk production in the mammary glands by promoting the proliferation and differentiation of mammary epithelial cells. Additionally Prolactin influences reproductive functions metabolism and immune regulation. It is not part of a larger protein complex but interacts with various proteins to perform its functions. Notably it modulates the immune response contributing to tolerance during pregnancy and affecting the balance between pro-inflammatory and anti-inflammatory cytokines.

Pathways

Prolactin plays important roles in the Jak-STAT and MAPK signaling pathways. In these pathways it shares interactions with related proteins such as Growth Hormone and Prolactin Receptor aiding in cell growth differentiation and metabolism regulation. Prolactin's involvement in these pathways highlights its central role in physiological processes linking various hormonal signals to regulate body functions efficiently.

Prolactin's imbalance can lead to conditions such as hyperprolactinemia and prolactinomas both characterized by excessive levels of this hormone in the blood. These conditions are often related to alterations in the pituitary gland function. Prolactin interacts closely with Dopamine a neurotransmitter that inhibits its secretion influencing both its production and release. Dysregulation of this interaction may contribute to the development of these disorders impacting endocrine health and overall well-being.
style
left-column

Product protocols

style
left-column

Target data

Prolactin acts primarily on the mammary gland by promoting lactation.
See full target information Prl
style
left-column

Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Biology open 12: PubMed37843404

2023

Impact of high-fat diet and exposure to constant light on reproductive competence of female ICR mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kelsey Teeple,Prabha Rajput,Sara Scinto,Jenna Schoonmaker,Corrin Davis,Michayla Dinn,Mackenzie McIntosh,Sairam Krishnamurthy,Karen Plaut,Theresa Casey

Frontiers in physiology 13:886298 PubMed35770190

2022

Impact of Chronic Prenatal Stress on Maternal Neuroendocrine Function and Embryo and Placenta Development During Early-to-Mid-Pregnancy in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Neta Gotlieb,Kathryn Wilsterman,Samantha L Finn,Madison F Browne,Savannah R Bever,Eiko Iwakoshi-Ukena,Kazuyoshi Ukena,George E Bentley,Lance J Kriegsfeld

Brain : a journal of neurology 145:2894-2909 PubMed35325034

2022

Dysregulation of serum prolactin links the hypothalamus with female nociceptors to promote migraine.

Applications

Unspecified application

Species

Unspecified reactive species

Moe Watanabe,Caroline M Kopruszinski,Aubin Moutal,Daigo Ikegami,Rajesh Khanna,Yanxia Chen,Sarah Ross,Kimberly Mackenzie,Jennifer Stratton,David W Dodick,Edita Navratilova,Frank Porreca

Developmental psychobiology 64:e22264 PubMed35312052

2022

Maternal prolactin levels during late pregnancy and nurturing behavior of offspring in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shinnosuke Masuda,Oh Kwan Ee,Taku James Sairenji,Seika Sato,Hiroyuki Yajima,Izuki Amano,Noriyuki Koibuchi,Noriaki Shimokawa

Neuroendocrinology 112:796-806 PubMed34666336

2021

Prolactin Reduces Hippocampal Parvalbumin and GABAA Receptor Expression in Female Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Susana Mellado,Beatriz Moreno-Ruiz,Sara Expósito,Miriam Fernández,Eduardo D Martín

Andrology 9:1652-1661 PubMed33998165

2021

Hyperprolactinemia in a male pituitary androgen receptor knockout mouse is associated with female-like lactotroph development.

Applications

Unspecified application

Species

Unspecified reactive species

Laura O'Hara,Helen C Christian,Paul Le Tissier,Lee B Smith

Cell death & disease 12:588 PubMed34099636

2021

Oxytocin receptor induces mammary tumorigenesis through prolactin/p-STAT5 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Mingjun San,Jing Zhang,Anlan Yang,Wanhua Xie,Yang Chen,Xiaodan Lu,Yuntao Zhang,Mingyue Zhao,Xuechao Feng,Yaowu Zheng

The EMBO journal 40:e104267 PubMed33491217

2021

Activation of the medial preoptic area (MPOA) ameliorates loss of maternal behavior in a Shank2 mouse model for autism.

Applications

Unspecified application

Species

Unspecified reactive species

Stefanie Grabrucker,Jessica Pagano,Johanna Schweizer,Carolina Urrutia-Ruiz,Michael Schön,Kevin Thome,Günter Ehret,Andreas M Grabrucker,Rong Zhang,Bastian Hengerer,Jürgen Bockmann,Chiara Verpelli,Carlo Sala,Tobias M Boeckers

Endocrinology 162: PubMed33248443

2020

MicroRNA-7a2 Regulates Prolactin in Developing Lactotrophs and Prolactinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mary P LaPierre,Svenja Godbersen,Mònica Torres Esteban,Anaïs Nura Schad,Mathias Treier,Umesh Ghoshdastider,Markus Stoffel

Neuroendocrinology 111:805-830 PubMed32645699

2020

Maternal Motivation: Exploring the Roles of Prolactin and Pup Stimuli.

Applications

Unspecified application

Species

Unspecified reactive species

Hugo Salais-López,María Abellán-Álvaro,María Bellés,Enrique Lanuza,Carmen Agustin-Pavon,Fernando Martínez-García
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Alternative Products

Assay Kits

AB214572

Mouse Prolactin/PRL ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (AB214572)

  • 0
  • 0

Complementary Products

Assay Kits

AB199082

Mouse Leptin ELISA Kit

SimpleStep

ELISA - Mouse Leptin ELISA Kit (AB199082)

  • 0
  • 0
Assay Kits

AB263880

Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone)

Recombinant|SimpleStep

ELISA - Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (AB263880)

  • 0
  • 0
Assay Kits

AB260064

Human C-Peptide ELISA Kit

Recombinant|SimpleStep

ELISA - Human C-Peptide ELISA Kit (AB260064)

  • 0
  • 0
Primary Antibodies

AB2772

Anti-Prolactin Receptor/PRL-R antibody [U5]

Flow Cytometry - Anti-Prolactin Receptor/PRL-R antibody [U5] (AB2772)

  • 3
  • 3

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com