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AB100732

Mouse proMMP9 ELISA Kit

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(5 Publications)

Mouse proMMP9 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse proMMP9 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Clg4b, Mmp9, Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB

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Sandwich ELISA - Mouse proMMP9 ELISA Kit (AB100732)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse proMMP9 ELISA Kit (AB100732)

Representative standard curve using ab100732

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 50 pg/mL

Range

74.07 - 18000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

proMMP9 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100732) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse proMMP9 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse proMMP9 coated on a 96-well plate. Standards and samples are pipetted into the wells and proMMP9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse proMMP9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of proMMP9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "96 - 118 %", "average": "= 109.7" }, { "sample": "Serum", "range": "97 - 132 %", "average": "= 114.4" }, { "sample": "Plasma", "range": "101 - 121 %", "average": "= 112" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100732-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"proMMP9 Microplate (12 x 8 wells)", "number":"AB100732-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100732-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100732-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100732-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse proMMP9 Standard (lyophilized)", "number":"AB100732-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100732-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse proMMP9", "number":"AB100732-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100732-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MMP-9 protein also known as matrix metalloproteinase-9 or gelatinase B functions as an enzyme involved in the breakdown of extracellular matrix proteins. It consists of a zinc ion at its active site and facilitates the degradation of type IV and V collagens fibronectin and elastin. MMP-9 has a molecular weight of approximately 92 kDa and is expressed in a variety of tissues including the liver lungs and immune cells. This enzyme can exist in monomer and dimer forms with its activity regulated by tissue inhibitors of metalloproteinases (TIMPs).
Biological function summary

Matrix metalloproteinase-9 profoundly influences tissue remodeling inflammation and angiogenesis. It does not form part of a larger protein complex but interacts closely with substrates in the extracellular matrix. MMP-9 plays important roles in processes such as embryogenesis reproduction and wound healing through the remodeling of surrounding tissues. The MMP-9 protein is often measured using assays like ELISA or analyzed via techniques such as Western blotting to determine its expression levels and activity in different biological contexts.

Pathways

MMP-9 participates in the matrix metalloproteinase pathway and the NF-kB signaling pathway. These pathways are essential in orchestrating processes such as tissue remodeling and inflammatory responses. MMP-9 acts alongside other matrix metalloproteinases including MMP-2 and is regulated by factors like cytokines growth factors and hormones ensuring the precision of extracellular matrix degradation during physiological processes.

Matrix metalloproteinase-9 has associations with cancer invasion and metastasis as well as chronic inflammatory diseases like rheumatoid arthritis. In cancer upregulated MMP-9 expression aids tumor cells in breaching the basement membrane facilitating metastasis. The disorder-associated pathways often involve interactions with proteins like vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-beta). Understanding MMP-9's role in these conditions can contribute to developing targeted therapies that inhibit its activity potentially mitigating disease progression.
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Product protocols

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Target data

Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (By similarity). Could play a role in bone osteoclastic resorption (PubMed : 8132709). Cleaves KiSS1 at a Gly-|-Leu bond (By similarity). Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed : 23142597, PubMed : 32883094). Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide (By similarity).
See full target information Mmp9
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 12:623256 PubMed34381441

2021

A20 Establishes Negative Feedback With TRAF6/NF-κB and Attenuates Early Brain Injury After Experimental Subarachnoid Hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Ji Deng,QuZhen Deji,WangDui Zhaba,Jia-Qiang Liu,Sheng-Qing Gao,Yan-Ling Han,Meng-Liang Zhou,Chun-Xi Wang

Journal of molecular endocrinology 66:141-155 PubMed33350981

2020

Dysregulated healing responses in diabetic wounds occur in the early stages postinjury.

Applications

Unspecified application

Species

Unspecified reactive species

Kiara Boodhoo,Mare Vlok,David L Tabb,Kathryn H Myburgh,Mari van de Vyver

International journal of molecular medicine 40:1869-1880 PubMed29039441

2017

Rivaroxaban attenuates thrombosis by targeting the NF-κB signaling pathway in a rat model of deep venous thrombus.

Applications

Unspecified application

Species

Unspecified reactive species

Junhao Ma,Xinxi Li,Yang Wang,Zhenwei Yang,Jun Luo

Scientific reports 7:597 PubMed28377603

2017

HMGB1-TLR4-IL23-IL17A axis promotes paraquat-induced acute lung injury by mediating neutrophil infiltration in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Bailing Yan,Feng Chen,Lijun Xu,Jihong Xing,Xuefu Wang

International journal of oral science 7:220-31 PubMed26674424

2015

The effects of interleukin-1β in modulating osteoclast-conditioned medium's influence on gelatinases in chondrocytes through mitogen-activated protein kinases.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xie,Na Fu,Lin-Yi Cai,Tao Gong,Guo Li,Qiang Peng,Xiao-Xiao Cai
View all publications
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