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AB315297

Mouse PSAP ELISA Kit

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Mouse PSAP ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse PSAP with a sensitivity of 13.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Sgp1, Psap, Prosaposin, Sulfated glycoprotein 1, SGP-1

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Sandwich ELISA - Mouse PSAP ELISA Kit (AB315297)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PSAP ELISA Kit (AB315297)

Example of mouse prosaposin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse PSAP ELISA Kit (AB315297)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PSAP ELISA Kit (AB315297)

Interpolated concentration of native prosaposin was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 1,000 serum, plasma (citrate), plasma (EDTA), and plasma (heparin).  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse PSAP ELISA Kit (AB315297)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse PSAP ELISA Kit (AB315297)

Interpolated concentration of native prosaposin was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 20 NIH/3T3 cell culture supernatant.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 13.4 pg/mL

Range

43.75 - 2800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse PSAP SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PSAP protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Mouse PSAP with 13.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.8" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.1" } ]
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Recovery

[ { "sample": "Serum", "range": "101 - 116 %", "average": "= 108" }, { "sample": "EDTA Plasma", "range": "91 - 102 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "96 - 100 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "88 - 92 %", "average": "= 91" }, { "sample": "Cell culture supernatant", "range": "91 - 103 %", "average": "= 97" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315297-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315297-CMP08", "productcode":"" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB315297-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB315297-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse PSAP Detector Antibody", "number":"AB315297-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse PSAP Lyophilized Recombinant Protein", "number":"AB315297-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB315297-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315297-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315297-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB315297-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse PSAP Capture Antibody", "number":"AB315297-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Prosaposin. Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.. Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.. Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.. Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12).. Saposins are specific low-molecular mass non-enzymatic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases.
See full target information Psap
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websiteProtocolBooklet
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