AB245713

Mouse PTX3 ELISA Kit

Recombinant
SimpleStep
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(3 Publications)

Mouse PTX3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse PTX3 with a sensitivity of 22.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Tsg14, Ptx3, Pentraxin-related protein PTX3, Pentaxin-related protein PTX3, Tumor necrosis factor-inducible gene 14 protein, TSG-14

3 Images

Sandwich ELISA - Mouse PTX3 ELISA Kit (AB245713)

sELISA

Supplier Data

Sandwich ELISA - Mouse PTX3 ELISA Kit (AB245713)

Mouse PTX3 Standard Curve Comparison. Standard Curve comparison between mouse PTX3 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Mouse PTX3 ELISA Kit (AB245713)

Example of mouse PTX3 standard curve in Sample Diluent NS. The PTX3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse PTX3 ELISA Kit (AB245713)

Interpolated concentrations of native PTX3 in mouse serum, plasma, and cell culture supernatant samples. The concentrations of PTX3 were measured in duplicates, interpolated from the PTX3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 12.5%, plasma (EDTA) 12.5%, plasma (heparin) 12.5% and 3T3-L1 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PTX3 concentration was determined to be 36.40 ng/mL in serum, 33.63 ng/mL in plasma (citrate), 50.51 ng/mL in plasma (EDTA), 51.03 ng/mL in plasma (heparin), and 8.37 ng/mL in 3T3-L1 cell culture supernatant.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 22.3 pg/mL

Range

156.25 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse PTX3 ELISA Kit (ab245713) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PTX3 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PTX3 with 22.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "100 - 105 %", "average": "= 102" }, { "sample": "Serum", "range": "95 - 111 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "108 - 120 %", "average": "= 113" }, { "sample": "Heparin Plasma", "range": "105 - 108 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "106 - 110 %", "average": "= 108" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab245713-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB245713-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB245713-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB245713-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB245713-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB245713-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB245713-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse PTX3 Lyophilized Recombinant Protein", "number":"AB245713-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse PTX3 Capture Antibody", "number":"AB245713-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse PTX3 Detector Antibody", "number":"AB245713-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB245713-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pentraxin 3 (PTX3) also known as TSG-14 or TNF-Inducible Gene 14 Protein is an important component of the immune system. Mechanically PTX3 functions as part of the body's defense mechanism against pathogens and tissue damage. It has a molecular weight of approximately 42 kDa and is expressed in a range of tissues such as the immune cells endothelium and epithelial cells. Upon inflammatory signals these cells secrete PTX3 which quickly identifies microbial molecules and apoptotic cells.

Biological function summary

The role of PTX3 extends beyond simple pathogen recognition. It is involved in the regulation of inflammation and tissue homeostasis. As part of the pentraxin superfamily PTX3 plays a role in the formation of complexes with other molecules such as complement components. These interactions modulate the complement cascade an essential part of the innate immune response and influence the resolution of inflammation. PTX3 also interacts with extracellular matrix components stabilizing tissue structure during immune response.

Pathways

PTX3 integrates into vital immune signaling networks. It actively participates in the complement pathway where it regulates the activity of C1q and C3 key complement proteins. Additionally PTX3 connects with the Toll-like receptor (TLR) signaling pathway. This interaction helps modulate the body's response to microbial infections by influencing the activity of TLR4 among other related proteins which are fundamental in recognizing pathogen-associated molecular patterns.

PTX3 is linked to various inflammatory conditions. High levels of PTX3 often indicate a response to cardiovascular diseases such as atherosclerosis. In this context its interactions with C1q highlight its role in modulating disease progression. PTX3 also relates to autoimmune diseases like rheumatoid arthritis where its increased expression correlates with disease severity. In rheumatoid arthritis PTX3 can interact with pro-inflammatory cytokines like TNF-alpha exacerbating the inflammatory process.

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Product protocols

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Target data

Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.

See full target information Ptx3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 19:4657-4671 PubMed37781037

2023

Pgam5-mediated PHB2 dephosphorylation contributes to endotoxemia-induced myocardial dysfunction by inhibiting mitophagy and the mitochondrial unfolded protein response.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Cai,Ziying Li,Zemao Zheng,Zhongzhou Guo,Qian Li,Shuxian Deng,Nengxian Shi,Qing Ou,Hao Zhou,Zhigang Guo,Zhongqing Chen,Hang Zhu

Cancer science 113:4311-4326 PubMed36074525

2022

Pentraxin 3 is an adipose tissue-related serum marker for pancreatic cancer cachexia predicting subsequent muscle mass and visceral fat loss.

Applications

Unspecified application

Species

Unspecified reactive species

Katsuhiko Sato,Hayato Hikita,Minoru Shigekawa,Seiya Kato,Yoichi Sasaki,Kazuma Shinkai,Makoto Fukuoka,Shinnosuke Kudo,Yu Sato,Kenji Fukumoto,Kumiko Shirai,Yuta Myojin,Sadatsugu Sakane,Kazuhiro Murai,Teppei Yoshioka,Akira Nishio,Takahiro Kodama,Ryotaro Sakamori,Tomohide Tatsumi,Tetsuo Takehara

Brain communications 4:fcac235 PubMed36196088

2022

The reduction of astrocytic tau prevents amyloid-β-induced synaptotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo Cisternas,Xavier Taylor,Pablo Martinez,Orlando Maldonado,Nur Jury,Cristian A Lasagna-Reeves

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse PTX3 ELISA Kit

Sandwich ELISA - Mouse PTX3 ELISA Kit (AB245713)

Sandwich ELISA - Mouse PTX3 ELISA Kit (AB245713)

Sandwich ELISA - Mouse PTX3 ELISA Kit (AB245713)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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Primary Antibodies

Primary Antibodies

Product promise