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Mouse RANKL ELISA Kit (TNFSF11) is a Sandwich (quantitative) ELISA kit for the measurement of Mouse RANKL (TNFSF11) in Mouse in Plasma, Cell culture supernatant, Serum samples.


Images

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Mouse

Range

2.74 - 2000 pg/mL

Sensitivity

< 4 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (By similarity). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts (PubMed:24039232). During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (PubMed:23395171, PubMed:26644563).

Alternative names

What's included?

1 x 96 Tests
Components
200X HRP-Streptavidin Concentrate
1 x 200 µL
20X Wash Buffer
1 x 25 mL
5X Assay Diluent B
1 x 15 mL
Assay Diluent A
1 x 30 mL
Biotinylated anti-Mouse RANKL
2 x 1 Vial
RANKL Microplate (12 strips x 8 wells)
1 x 1 Unit
Recombinant Mouse RANKL Standard (lyophilized)
2 x 1 Vial
Stop Solution
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

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Mouse RANKL ELISA Kit (TNFSF11) is a Sandwich (quantitative) ELISA kit for the measurement of Mouse RANKL (TNFSF11) in Mouse in Plasma, Cell culture supernatant, Serum samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Mouse

Range

2.74 - 2000 pg/mL

Assay Platform

Microplate

Sensitivity

< 4 pg/mL

General recovery

96%

Recovery

Sample specific recovery

Sample type

Cell culture supernatant

Average %

= 106.5

Range

95 - 115 %

Sample type

Serum

Average %

= 95.05

Range

83 - 104 %

Sample type

Plasma

Average %

= 89.2

Range

74 - 104 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Abcam's RANKL Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse RANKL in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Mouse RANKL coated on a 96- well plate. Standards and samples are pipetted into the wells and RANKL present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse RANKL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RANKL bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Mouse RANKL ELISA Kit (TNFSF11) (ab100749), expandable thumbnail

    Sandwich ELISA - Mouse RANKL ELISA Kit (TNFSF11) (ab100749)

    RANKL measured in biological fluids showing quantity (pg) per mL of tested sample

  • Sandwich ELISA - Mouse RANKL ELISA Kit (TNFSF11) (ab100749), expandable thumbnail

    Sandwich ELISA - Mouse RANKL ELISA Kit (TNFSF11) (ab100749)

    Representative Standard Curve using ab100749

  • Sandwich ELISA - Mouse RANKL ELISA Kit (TNFSF11) (ab100749), expandable thumbnail

    Sandwich ELISA - Mouse RANKL ELISA Kit (TNFSF11) (ab100749)

    Representative Standard Curve using ab100749

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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