Mouse RANKL ELISA Kit (TNFSF11) is a sandwich ELISA designed to quantify Mouse RANKL (TNFSF11) with a sensitivity of 4 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 2.74 - 2000 pg/mL
- Cited in over 20 publications
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Publications
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- Frontiers in endocrinology 14:12075022023Azilsartan inhibits inflammation-triggered bone resorption and osteoclastogenesis via suppression of TNF-α expression in macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiqiu Fan,Hideki Kitaura,Jiayi Ren,Fumitoshi Ohori,Takahiro Noguchi,Aseel Marahleh,Jinghan Ma,Kayoko Kanou,Mariko Miura,Kohei Narita,Angyi Lin,Itaru MizoguchiPubMed 37795376
- iScience 26:1064702023An inducible explant model of osteoclast-osteoprogenitor coordination in exacerbated osteoclastogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJarred M Whitlock,Luis F de Castro,Michael T Collins,Leonid V Chernomordik,Alison M BoycePubMed 37091244
- Bioengineered 13:13632-136422022Plumbagin relieves rheumatoid arthritis through nuclear factor kappa-B (NF-κB) pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChang Shu,Jun Chen,Meiyan Lv,Yiyuan Xi,Jujia Zheng,Xiangwei XuPubMed 35653787
- Drug delivery 29:889-9052022Bone-targeted nanoplatform enables efficient modulation of bone tumor microenvironment for prostate cancer bone metastasis treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangyu Zhang,Qingbin Liu,Tingting Zhang,Pei Gao,Hui Wang,Lu Yao,Jingwen Huang,Shulong JiangPubMed 35285760
- Bone research 10:122022IκB-ζ signaling promotes chondrocyte inflammatory phenotype, senescence, and erosive joint pathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManoj Arra,Gaurav Swarnkar,Yael Alippe,Gabriel Mbalaviele,Yousef Abu-AmerPubMed 35145063
- Nature communications 13:7712022An optogenetic approach for regulating human parathyroid hormone secretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunhui Liu,Lu Zhang,Nan Hu,Jie Shao,Dazhi Yang,Changshun Ruan,Shishu Huang,Liping Wang,William W Lu,Xinzhou Zhang,Fan YangPubMed 35140213
- International journal of molecular sciences 23:2021Intermittent Hypoxic Therapy Inhibits Allogenic Bone-Graft Resorption by Inhibition of Osteoclastogenesis in a Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatasja Leth Bergholt,Ari Demirel,Michael Pedersen,Ming Ding,Tue Wenzel Kragstrup,Thomas Andersen,Bent Winding Deleuran,Casper Bindzus FoldagerPubMed 35008749
- Journal of biomedical science 28:742021Restoration of 5-methoxytryptophan protects against atherosclerotic chondrogenesis and calcification in ApoE mice fed high fat diet.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuan-Lin Lee,Tsai-Lien Liao,Jing-Yiing Wu,Kenneth K Wu,Cheng-Chin KuoPubMed 34749728
- EMBO reports 22:e524812021RANKL from bone marrow adipose lineage cells promotes osteoclast formation and bone loss.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Hu,Xiaoqun Li,Xin Zhi,Wei Cong,Biaotong Huang,Huiwen Chen,Yajun Wang,Yinghua Li,Lipeng Wang,Chao Fang,Jiawei Guo,Ying Liu,Jin Cui,Liehu Cao,Weizong Weng,Qirong Zhou,Sicheng Wang,Xiao Chen,Jiacan SuPubMed 34121311
- JBMR plus 4:e104132020Targeting Bone Cells During Sexual Maturation Reveals Sexually Dimorphic Regulation of Endochondral Ossification.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeather Fairfield et. al.PubMed 33210065
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 2.74 - 2000 pg/mL
- Sensitivity
- < 4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor (PubMed:22437732). Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (By similarity). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts (PubMed:18586671, PubMed:24039232, PubMed:27336669). During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (PubMed:23395171, PubMed:26644563).
Alternative names
CD254, Opgl, Rankl, Trance, Tnfsf11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF, OPGL, RANKL, TRANCE
What's included?
Recommended products
Mouse RANKL ELISA Kit (TNFSF11) is a sandwich ELISA designed to quantify Mouse RANKL (TNFSF11) with a sensitivity of 4 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 2.74 - 2000 pg/mL
- Cited in over 20 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 2.74 - 2000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 4 pg/mL
- General recovery
- 96%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 106.5 | Range 95 - 115 % |
Sample type Serum | Average % = 95.05 | Range 83 - 104 % |
Sample type Plasma | Average % = 89.2 | Range 74 - 104 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's RANKL Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse RANKL in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse RANKL coated on a 96- well plate. Standards and samples are pipetted into the wells and RANKL present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse RANKL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RANKL bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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3 product images
Sandwich ELISA - Mouse RANKL ELISA Kit (TNFSF11) (ab100749)
RANKL measured in biological fluids showing quantity (pg) per mL of tested sample
Sandwich ELISA - Mouse RANKL ELISA Kit (TNFSF11) (ab100749)
Representative Standard Curve using ab100749
Sandwich ELISA - Mouse RANKL ELISA Kit (TNFSF11) (ab100749)
Representative Standard Curve using ab100749
Downloads
Product protocols
- Visit the general protocols
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