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Mouse RANKL ELISA Kit (TNFSF11) is a Sandwich (quantitative) ELISA kit for the measurement of Mouse RANKL (TNFSF11) in Mouse in Plasma, Cell culture supernatant, Serum samples.
Alternative names=Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF, OPGL, RANKL, TRANCE, Rankl, Opgl, Trance, Tnfsf11
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
2.74 - 2000 pg/mL
- Sensitivity
< 4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Mouse RANKL ELISA Kit (TNFSF11) is a Sandwich (quantitative) ELISA kit for the measurement of Mouse RANKL (TNFSF11) in Mouse in Plasma, Cell culture supernatant, Serum samples.
Alternative names=Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF, OPGL, RANKL, TRANCE, Rankl, Opgl, Trance, Tnfsf11
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
2.74 - 2000 pg/mL
- Assay Platform
Microplate
- Sensitivity
< 4 pg/mL
- General recovery
96%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 106.5 | Range 95 - 115 % |
Sample type Serum | Average % = 95.05 | Range 83 - 104 % |
Sample type Plasma | Average % = 89.2 | Range 74 - 104 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Abcam's RANKL Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse RANKL in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse RANKL coated on a 96- well plate. Standards and samples are pipetted into the wells and RANKL present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse RANKL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RANKL bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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