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Mouse RANTES ELISA Kit (CCL5) is a sandwich ELISA designed to quantify Mouse RANTES (CCL5) with a sensitivity of 1 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.82 - 200 pg/mL

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Images

Sandwich ELISA - Mouse RANTES ELISA Kit (CCL5) (AB100739), expandable thumbnail
  • Sandwich ELISA - Mouse RANTES ELISA Kit (CCL5) (AB100739), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture supernatant, Serum
Assay type
Sandwich (quantitative)
Reactive species
Mouse
Range
0.82 - 200 pg/mL
Sensitivity
< 1 pg/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.

Alternative names

What's included?

1 x 96 Tests
Components
200X HRP-Streptavidin Concentrate
1 x 200 µL
20X Wash Buffer
1 x 25 mL
5X Assay Diluent B
1 x 15 mL
Assay Diluent A
1 x 30 mL
Assay Diluent C
1 x 30 mL
Biotinylated anti-Mouse RANTES
2 x 1 Vial
RANTES Microplate (12 x 8 wells)
1 x 1 Unit
Recombinant Mouse RANTES Standard (lyophilized)
2 x 1 Vial
Stop Solution
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Mouse RANTES ELISA Kit (CCL5) is a sandwich ELISA designed to quantify Mouse RANTES (CCL5) with a sensitivity of 1 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.82 - 200 pg/mL

Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture supernatant, Serum
Assay type
Sandwich (quantitative)
Reactive species
Mouse
Range
0.82 - 200 pg/mL
Assay Platform
Microplate
Sensitivity
< 1 pg/mL
General recovery
100%

Recovery

Sample specific recovery

Sample type
Cell culture supernatant
Average %
= 114.7
Range
86 - 122 %
Sample type
Serum
Average %
= 109.9
Range
98 - 119 %
Sample type
Plasma
Average %
= 75.41
Range
68 - 87 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Abcam's RANTES Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse RANTES in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse RANTES coated on a 96-well plate. Standards and samples are pipetted into the wells and RANTES present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse RANTES antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RANTES bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get results in 90 minutes with Mouse RANTES ELISA Kit (ab215537) from our SimpleStep ELISA® range.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Mouse RANTES ELISA Kit (CCL5) (ab100739), expandable thumbnail

    Sandwich ELISA - Mouse RANTES ELISA Kit (CCL5) (ab100739)

    Representative standard curve using ab100739

  • Sandwich ELISA - Mouse RANTES ELISA Kit (CCL5) (ab100739), expandable thumbnail

    Sandwich ELISA - Mouse RANTES ELISA Kit (CCL5) (ab100739)

    Representative standard curve using ab100739

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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