Mouse RANTES ELISA Kit (CCL5) is a sandwich ELISA designed to quantify Mouse RANTES (CCL5) with a sensitivity of 1 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.82 - 200 pg/mL
Application | Reactivity | Dilution info | Notes |
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Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
Scya5, Ccl5, C-C motif chemokine 5, MuRantes, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES
Mouse RANTES ELISA Kit (CCL5) is a sandwich ELISA designed to quantify Mouse RANTES (CCL5) with a sensitivity of 1 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.82 - 200 pg/mL
Sample type | Average % | Range |
---|---|---|
Sample type Cell culture supernatant | Average % = 114.7 | Range 86 - 122 % |
Sample type Serum | Average % = 109.9 | Range 98 - 119 % |
Sample type Plasma | Average % = 75.41 | Range 68 - 87 % |
Abcam's RANTES Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse RANTES in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse RANTES coated on a 96-well plate. Standards and samples are pipetted into the wells and RANTES present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse RANTES antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RANTES bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Mouse RANTES ELISA Kit (ab215537) from our SimpleStep ELISA® range.
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Representative standard curve using ab100739
Representative standard curve using ab100739
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