JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB325629

Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent is a 55 min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse/Rat ACTH (Adrenocorticotropic Hormone) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range : 2.06 - 1500 pg/ml with a sensitivity of 1.35 pg/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent pending
5 Images
Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)
  • sELISA

Lab

Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)
  • sELISA

Lab

Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)

Standard curve comparison between the Chemiluminescent Mouse/Rat ACTH SimpleStep ELISA and colorimetric Mouse/Rat ACTH SimpleStep ELISA (ab263880) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)
  • sELISA

Lab

Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)

Dynamic range comparison between the Chemiluminescent Mouse/Rat ACTH SimpleStep ELISA and colorimetric Mouse/Rat ACTH SimpleStep ELISA (ab263880), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)
  • sELISA

Lab

Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)

Interpolated concentrations of native ACTH in mouse serum and plasma samples. The concentrations of ACTH were measured in duplicates, interpolated from the ACTH standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean ACTH concentration was determined to be 123 pg/mL in serum, 433 pg/mL in plasma (citrate), 240 pg/mL in plasma (heparin) and 328 pg/mL in plasma (EDTA).

Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)
  • sELISA

Lab

Sandwich ELISA - Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (AB325629)

Interpolated concentrations of native ACTH in rat serum and plasma samples. The concentrations of ACTH were measured in duplicates, interpolated from the ACTH standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 15%, plasma (heparin) 15% and plasma (EDTA) 15%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean ACTH concentration was determined to be 1,178 pg/mL in serum, 2,975 pg/mL in plasma (citrate), 1,988 pg/mL in plasma (heparin) and 1,508 pg/mL in plasma (EDTA).

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma

Reacts with

Mouse, Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.35 pg/mL

Range

2.06 - 1500 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (ab325629) is a rapid single-wash sandwich 55 min ELISA to measure Mouse/Rat ACTH (Adrenocorticotropic Hormone) in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma with a sensitivity of 1.35 pg/mL.

How the assay works

Mouse/Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit, Chemiluminescent (ab325629) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse/Rat ACTH (Adrenocorticotropic Hormone) (ab325629) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-ACTH antibody [EPR20361-248] - BSA and Azide free (Capture) ab259687
- Anti-ACTH antibody [EPR20361-235] - BSA and Azide free (Detector) ab259688
style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.2" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.6" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "82 - 92 %", "average": "= 87" }, { "sample": "Citrate plasma", "range": "89 - 107 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "100 - 110 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "113 - 120 %", "average": "= 116" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325629-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Mouse/Rat ACTH (Adrenocorticotropic Hormone) Lyophilized Peptide Protein", "number":"AB325629-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse/Rat ACTH (Adrenocorticotropic Hormone) Detector Antibody", "number":"AB325629-CMP02", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325629-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB325629-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325629-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB325629-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse/Rat ACTH (Adrenocorticotropic Hormone) Capture Antibody", "number":"AB325629-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325629-CMP09", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325629-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325629-CMP10", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

See full target information ACTH
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com