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AB263880

Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone)

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(31 Publications)

Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) is a single-wash 90-min Simplestep used to quantify Mouse/Rat ACTH (adrenocorticotropic hormone) with a sensitivity of 6 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

Adrenocorticotropic hormone, Adrenocorticotropin, Alpha melanocyte stimulating hormone, Beta LPH, Beta melanocyte stimulating hormone, Beta-endorphin, Corticotropin lipotropin, Corticotropin-like intermediary peptide, Gamma LPH, Lipotropin beta, Lipotropin gamma, Lipotropin, included, MSH, Melanocyte-stimulating hormone, included, Melanotropin alpha, Melanotropin beta, Melanotropin gamma, Melanotropin, included, Met-enkephalin, NPP, Opiomelanocortin prepropeptide, POC, POMC, Pomc-1, Pomc2, Pro ACTH endorphin, Pro opiomelanocortin, Pro-opiomelanocortin-alpha, Proopiomelanocortin preproprotein, Tetracosactide, alpha-MSH, beta-MSH, gamma-MSH

4 Images
ELISA - Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (AB263880)
  • ELISA

Supplier Data

ELISA - Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (AB263880)
Sandwich ELISA - Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (AB263880)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (AB263880)

Interpolated concentrations of native ACTH in mouse and rat serum and plasma samples.

The concentrations of ACTH were measured in duplicates, interpolated from the ACTH standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (edta) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACTH concentration was determined to be 123.02 pg/mL in serum, 433.40 pg/mL in plasma (citrate), 240.48 pg/mL in plasma (heparin) and 328.43 pg/mL in plasma (edta).

Sandwich ELISA - Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (AB263880)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (AB263880)

Interpolated concentrations of native ACTH in rat serum and plasma samples.

The concentrations of ACTH were measured in duplicates, interpolated from the ACTH standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 15%, plasma (heparin) 15% and plasma (edta) 15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACTH concentration was determined to be 1178.40 pg/mL in serum, 2974.54 pg/mL in plasma (citrate), 1988.24 pg/mL in plasma (heparin) and 1508.42 pg/mL in plasma (edta).

Sandwich ELISA - Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (AB263880)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (AB263880)

Example of mouse and rat ACTH standard curve in 1X Cell Extraction Buffer PTR.

The ACTH standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse, Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 6 pg/mL

Range

7.81 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) ab263880 is a rapid single-wash 90-min ELISA kit to measure Mouse/Rat ACTH in serum and plasma. This SimpleStep ELISA® sensitivity is 6 pg/mL.

How the assay works

Mouse/Rat ACTH SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes native mouse and rat ACTH in serum and plasma samples only. As ACTH is a product of POMC, this kit is expected to cross-react in any samples that contain the unprocessed precursor. Cell culture supernatant, cell and tissue extract samples have not been tested with this kit.

Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) ab263880 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Mouse/Rat ACTH ELISA kit ab263880

Mouse/Rat ACTH ELISA kit ab263880 has been used to measure the hypothalamus–pituitary–adrenal axis activation(1)(2)

References:

(1)Hoyoung Kim et al. 2023, PMID: 37653040,
(2)Weiyi Chen et al. 2023, PMID: 37075752

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Mouse/Rat ACTH (adrenocorticotropic hormone) Antibody Pair - BSA and Azide free ab267700

Related and recommended products

Mouse/Rat ELISA kit ab263880 is commonly used to measure the activity of the hypothalamic-pituitary-adrenal axis.

Other ELISA kits to measure related hormones include:

- Cortisol ELISA kit ab108665
- Human Growth Hormone ELISA kit ab190811

ACTH, adrenocorticotropic hormone, is a peptide hormone cleavage product of the POMC protein produced by the pituitary gland. ACTH plays an important role in stress and circadian rhythm signaling. ACTH's primary action is on the adrenal cortex, where its binding induces the secretion of glucocorticoid steroid hormones. The peptide sequence for this hormone is highly evolutionarily conserved amongst mammals. Specifically, for mouse and rat, this sequence shares 100% identity.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]
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Recovery

[ { "sample": "Serum", "range": "82 - 92 %", "average": "= 87" }, { "sample": "EDTA Plasma", "range": "100 - 110 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "113 - 120 %", "average": "= 116" }, { "sample": "Citrate plasma", "range": "89 - 107 %", "average": "= 101" } ]
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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab263880-10x96Tests", "assetComponentDetails": [ { "size":"10 x 2 Vial", "name":"Mouse/Rat ACTH (adrenocorticotropic hormone) Lyophilized Protein", "number":"AB263880-CMP11" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB263880-CMP10" }, { "size":"10 x 600 µL", "name":"10X Mouse/Rat ACTH (adrenocorticotropic hormone) Capture Antibody", "number":"AB263880-CMP07" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB263880-CMP06" }, { "size":"10 x 600 µL", "name":"10X Mouse/Rat ACTH (adrenocorticotropic hormone) Detector Antibody", "number":"AB263880-CMP08" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB263880-CMP03" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB263880-CMP02" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB263880-CMP04" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB263880-CMP05" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB263880-CMP09" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab263880-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB263880-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB263880-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB263880-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB263880-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB263880-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse/Rat ACTH (adrenocorticotropic hormone) Lyophilized Protein", "number":"AB263880-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB263880-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse/Rat ACTH (adrenocorticotropic hormone) Capture Antibody", "number":"AB263880-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB263880-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB263880-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse/Rat ACTH (adrenocorticotropic hormone) Detector Antibody", "number":"AB263880-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information ACTH
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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 7:1004-1017 PubMed40234625

2025

GDF15 links adipose tissue lipolysis with anxiety.

Applications

Unspecified application

Species

Unspecified reactive species

Logan K Townsend,Dongdong Wang,Carly M Knuth,Russta Fayyazi,Ahmad Mohammad,Léa J Becker,Evangelia E Tsakiridis,Eric M Desjardins,Zeel Patel,Celina M Valvano,Junfeng Lu,Alice E Payne,Ofure Itua,Kyle D Medak,Daniel M Marko,Jonathan D Schertzer,David C Wright,Shawn M Beaudette,Katherine M Morrison,André C Carpentier,Denis P Blondin,Rebecca E K MacPherson,Jordan G McCall,Marc G Jeschke,Gregory R Steinberg

iScience 28:111831 PubMed39967868

2025

Stimulation of TRPV1+ peripheral somatosensory nerves suppress inflammation via the somato-autonomic reflex.

Applications

Unspecified application

Species

Unspecified reactive species

Dengcen Song,Zheng Cao,Yong Hu,Fengyu Mao,Cheng Cao,Zijing Liu

Acta neuropathologica communications 13:22 PubMed39910602

2025

X-linked ubiquitin-specific peptidase 11 (USP11) increases susceptibility to Cushing's disease in women.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhang,Yanting Liu,Fang Liu,Kaiyu Guo,Runhua Tang,Jingwei Ye,Li Xue,Zhipeng Su,Zhe Bao Wu

Journal of neuroinflammation 21:302 PubMed39563331

2024

Microglia-derived ADAM9 promote GHRH neurons pyroptosis by Mad2L2-JNK-caspase-1 pathway in subarachnoid hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Mao,Yun Bao,Fan Liu,Qiyun Ye,Junxiang Peng,Jing Nie,Lijun Huang,Yonghong Liao,Yiheng Xing,Dongyang Wu,Ke Wang,Wenfeng Feng,Songtao Qi,Jun Pan,Binghui Qiu

Nature communications 15:8588 PubMed39362888

2024

Targeting osteoblastic 11β-HSD1 to combat high-fat diet-induced bone loss and obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanxin Zhong,Nanxi Li,Shengzheng Wang,Dijie Li,Zhihua Yang,Lin Du,Guangxin Huang,Haitian Li,Wing Sze Yeung,Shan He,Shuting Ma,Zhuqian Wang,Hewen Jiang,Huarui Zhang,Zhanghao Li,Xiaoxin Wen,Song Xue,Xiaohui Tao,Haorui Li,Duoli Xie,Yihao Zhang,Zefeng Chen,Junqin Wang,Jianfeng Yan,Zhengming Liang,Zongkang Zhang,Zhigang Zhong,Zeting Wu,Chao Wan,Chao Liang,Luyao Wang,Sifan Yu,Yuan Ma,Yuanyuan Yu,Fangfei Li,Yang Chen,Baoting Zhang,Aiping Lyu,Fuzeng Ren,Hong Zhou,Jin Liu,Ge Zhang

Biology of sex differences 15:75 PubMed39327618

2024

Sex-dependent effects of acute stress and alcohol exposure during adolescence on mRNA expression of brain signaling systems involved in reward and stress responses in young adult rats.

Applications

Unspecified application

Species

Unspecified reactive species

Carlotta Gobbi,Laura Sánchez-Marín,María Flores-López,Dina Medina-Vera,Francisco Javier Pavón-Morón,Fernando Rodríguez de Fonseca,Antonia Serrano

Endocrinology 165: PubMed39240718

2024

Androgens Suppress Corticosteroid Binding Globulin in Male Mice, Affecting the Endocrine Stress Response.

Applications

Unspecified application

Species

Unspecified reactive species

Vera Sommers,Max Gentenaar,Karel David,Nick Narinx,Vanessa Dubois,Jan Kroon,Frank Claessens,Onno C Meijer

eLife 12: PubMed39082939

2024

A kidney-hypothalamus axis promotes compensatory glucose production in response to glycosuria.

Applications

Unspecified application

Species

Unspecified reactive species

Tumininu S Faniyan,Xinyi Zhang,Donald A Morgan,Jorge Robles,Siresha Bathina,Paul S Brookes,Kamal Rahmouni,Rachel J Perry,Kavaljit H Chhabra

Cell reports 43:114395 PubMed38941187

2024

Macrophages preserve endothelial cell specialization in the adrenal gland to modulate aldosterone secretion and blood pressure.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Fan,Mara Karakone,Shunmugam Nagarajan,Nadine Nagy,Wiebke Mildenberger,Ekaterina Petrova,Laura Catharina Hinte,Mitchell Bijnen,Philipp Häne,Eric Nelius,Jing Chen,Irina Ferapontova,Ferdinand von Meyenn,Francesco Trepiccione,Mesut Berber,David Penton Ribas,Anne Eichmann,Maria-Christina Zennaro,Norihiko Takeda,Jens W Fischer,Ariadni Spyroglou,Martin Reincke,Felix Beuschlein,Johannes Loffing,Melanie Greter,Christian Stockmann

Lab animal 53:148-159 PubMed38806681

2024

Temperature-dependent differences in mouse gut motility are mediated by stress.

Applications

Unspecified application

Species

Unspecified reactive species

Alvin Han,Courtney Hudson-Paz,Beatriz G Robinson,Laren Becker,Amanda Jacobson,Julia A Kaltschmidt,Jennifer L Garrison,Ami S Bhatt,Denise M Monack
View all publications
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