AB324135

Mouse/Rat ADAMTS1 ELISA Kit- Extracellular

Recombinant
SimpleStep
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Mouse/Rat ADAMTS1 ELISA Kit- Extracellular is a Sandwich (quantitative) ELISA for the measurement of Mouse/Rat ADAMTS1 - Extracellular in Mouse, Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

A disintegrin and metalloproteinase with thrombospondin motifs 1, ADAM-TS 1, ADAM-TS1, ADAMTS-1, Adamts1

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Sandwich ELISA - Mouse/Rat ADAMTS1 ELISA Kit- Extracellular (AB324135)

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat ADAMTS1 ELISA Kit- Extracellular (AB324135)

Recombinant ADAMTS1 was spiked into the following rat samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 25% serum, plasma (citrate), plasma (EDTA), and CSF. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat ADAMTS1 ELISA Kit- Extracellular (AB324135)

Recombinant ADAMTS1 was spiked into the following mouse samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 25% serum, plasma (citrate), plasma (EDTA), and CSF. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat ADAMTS1 ELISA Kit- Extracellular (AB324135)

Example of mouse/rat ADAMTS1 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat ADAMTS1 ELISA Kit- Extracellular (AB324135)

Recombinant ADAMTS1 was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted sample is 50% cell culture media. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Cell culture media, Cerebral Spinal Fluid

Reacts with

Mouse, Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 54.225 pg/mL

Range

100 - 6400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse/Rat ADAMTS1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ADAMTS1 protein in Serum, Cit Plasma, EDTA Plasma, Cell culture media, Cerebral Spinal Fluid. Quantitate Mouse/Rat ADAMTS1 with 54.225 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

Singlewash protocol reduces assay time to 90 minutes or less.
High sensitivity, specificity and reproducibility from superior antibodies.
Fully validated in biological samples.
96wells plate breakable into 12 x 8 wells strips, available in 10pack (10 x 96well plates).

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.6" } ]

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Recovery

[ { "sample": "Serum", "range": "80 - 91 %", "average": "= 85" }, { "sample": "Citrate plasma", "range": "82 - 94 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "81 - 86 %", "average": "= 83" }, { "sample": "Cell culture media", "range": "100 - 110 %", "average": "= 104" }, { "sample": "Cerebral Spinal Fluid", "range": "90 - 103 %", "average": "= 96" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Cleaves aggrecan, a cartilage proteoglycan, at the '1691-Glu-|-Leu-1692' site (within the chondroitin sulfate attachment domain), and may be involved in its turnover. Has angiogenic inhibitor activity (By similarity). Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture (By similarity).

See full target information A disintegrin and metalloproteinase with thrombospondin motifs 1

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websiteProtocolBooklet

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Product promise

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