Mouse/Rat ADAMTS1 ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse/Rat ADAMTS1 - Intracellular in Cell Lysate, Tissue Extracts samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse, Rat
- Range
- 140.625 - 9000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 51.27 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cleaves aggrecan, a cartilage proteoglycan, at the '1691-Glu-|-Leu-1692' site (within the chondroitin sulfate attachment domain), and may be involved in its turnover. Has angiogenic inhibitor activity (By similarity). Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture (By similarity).
Alternative names
A disintegrin and metalloproteinase with thrombospondin motifs 1, ADAM-TS 1, ADAM-TS1, ADAMTS-1, Adamts1
What's included?
Mouse/Rat ADAMTS1 ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse/Rat ADAMTS1 - Intracellular in Cell Lysate, Tissue Extracts samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse, Rat
- Range
- 140.625 - 9000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 51.27 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 8 | C.V. = 4.7 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 3 | C.V. = 2.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 93 | Range 90 - 97 % |
Sample type Tissue Extracts | Average % = 101 | Range 98 - 106 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse/Rat ADAMTS1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ADAMTS1 protein in Cell Lysate, Tissue extracts. Quantitate Mouse/Rat ADAMTS1 with 51.27 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Singlewash protocol reduces assay time to 90 minutes or less.
- High sensitivity, specificity and reproducibility from superior antibodies.
- Fully validated in biological samples.
- 96wells plate breakable into 12 x 8 wells strips, available in 10pack (10 x 96well plates).
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Mouse/Rat ADAMTS1 ELISA Kit- Intracellular (ab324136)
Example of mouse/rat ADAMTS1 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse/Rat ADAMTS1 ELISA Kit- Intracellular (ab324136)
Interpolated concentration of native ADAMTS1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: NIH/3T3 cell extract 125 µg/mL, mouse kidney tissue extract 1,000 µg/mL, and rat lung tissue extract 1,000 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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