AB263879

Mouse/Rat B7-H4 ELISA Kit

Recombinant
SimpleStep
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Mouse/Rat B7-H4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse/Rat B7-H4 with a sensitivity of 21 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

B7h4, Vtcn1, V-set domain containing T-cell activation inhibitor 1, B7 homolog 4, Immune costimulatory protein B7-H4, Protein B7S1, T cell costimulatory molecule B7x, B7-H4

3 Images

Sandwich ELISA - Mouse/Rat B7-H4 ELISA Kit (AB263879)

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat B7-H4 ELISA Kit (AB263879)

Representative image of Mouse/Rat B7-H4 ELISA Kit (ab263879)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Mouse/Rat B7-H4 ELISA Kit Capture Antibody

- 10X Mouse/Rat B7-H4 Detector Antibody

- 10X Mouse/Rat B7-H4 ELISA Kit Lyophilised Purified Protein (2 vials)

- Mouse/Rat B7-H4 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat B7-H4 ELISA Kit (AB263879)

Interpolated concentrations of native B7-H4 in mouse uterus tissue extract and rat uterus tissue extract based on a 1,000 μg/mL and 750 μg/mL extract load.

The concentrations of B7-H4 were measured in duplicate and interpolated from the B7-H4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean B7-H4 concentration was determined to be 899.88 mg/mL in mouse uterus tissue extract and 1505.28 mg/mL in rat uterus tissue extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat B7-H4 ELISA Kit (AB263879)

Example of mouse B7-H4 standard curve in 1X Cell Extraction Buffer PTR.

The B7-H4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Tissue Culture Media

Reacts with

Mouse, Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 21 pg/mL

Range

46.88 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse/Rat B7-H4 ELISA Kit (ab263879) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of B7-H4 protein in tissue culture media and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat B7-H4 with 21 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

B7-H4 is a negative regulator of T-cell mediated immune response. Increased protein levels are associated with many forms of cancer, including ovarian, breast, lung, and prostate. In concert with Treg, B7-H4 plays a key role in suppression of tumor-associated antigen-specific T-cell immunity. The immunogen for this kit shares 99.1% and 91% sequence identity with the rat and human homologs, respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.5" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" } ]

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Recovery

[ { "sample": "Tissue Culture Media", "range": "93 - 104 %", "average": "= 100" }, { "sample": "Tissue Extracts", "range": "90 - 100 %", "average": "= 96" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab263879-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB263879-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB263879-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB263879-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB263879-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB263879-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB263879-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB263879-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse/Rat B7-H4 Capture Antibody", "number":"AB263879-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB263879-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse/Rat B7-H4 Lyophilized Recombinant Protein", "number":"AB263879-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse/Rat B7-H4 Detector Antibody", "number":"AB263879-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB263879-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

B7-H4 also known as VTCN1 or B7x is a member of the B7 family of immunoregulatory proteins. It has an approximate molecular mass of 50 kDa. B7-H4 expression is detected on the surface of various cells including B cells T cells monocytes and some cancer cells. Its expression is often inducible; immune cells can express B7-H4 in response to specific conditions or signals in the environment.

Biological function summary

B7-H4 acts as an immune checkpoint molecule that negatively regulates T cell immunity. It does not form a known complex with other proteins but instead interacts directly with T cells. By inhibiting T cell activation and proliferation B7-H4 contributes to the regulation of immune responses helping to maintain self-tolerance and prevent autoimmunity.

Pathways

B7-H4 is involved in the regulation of immune checkpoints prominently in the pathways related to immune tolerance and suppression. It plays a critical role in the pathways that modulate tumor evasion of the immune system. B7-H4 is related to other immune checkpoint proteins such as PD-L1 which are also pivotal in immune regulation and therapy targets.

B7-H4 is connected to cancer progression and autoimmune conditions. Overexpression of B7-H4 in certain cancers such as ovarian and breast cancer associates with tumor immune evasion allowing tumors to grow unchecked by the immune system. Its expression links with proteins like CTLA-4 in maintaining immune suppression. B7-H4 modifications could serve as a potential target in cancer immunotherapy aiming to enhance the immune system's ability to combat malignancies.

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Product protocols

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Target data

Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.

See full target information Vtcn1

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse/Rat B7-H4 ELISA Kit

Sandwich ELISA - Mouse/Rat B7-H4 ELISA Kit (AB263879)

Sandwich ELISA - Mouse/Rat B7-H4 ELISA Kit (AB263879)

Sandwich ELISA - Mouse/Rat B7-H4 ELISA Kit (AB263879)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise