AB227899

Mouse/Rat Bcl-xL ELISA Kit

Recombinant
SimpleStep
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(4 Publications)

Mouse/Rat Bcl-xL ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse/Rat Bcl-xL with a sensitivity of 28 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Bcl2l, Bclx, Bcl2l1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X

4 Images

Sandwich ELISA - Mouse/Rat Bcl-xL ELISA Kit (AB227899)

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat Bcl-xL ELISA Kit (AB227899)

Interpolated concentrations of native Bcl-xL in mouse and rat brain tissue extract samples based on a 300 μg/mL extract load.

The concentrations of Bcl-xL were measured in duplicate and interpolated from the Bcl-xL standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Bcl-xL concentration was determined to be 4.957 ng/mL in mouse brain tissue extract, and 3.067 ng/mL in rat brain tissue extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat Bcl-xL ELISA Kit (AB227899)

Interpolated concentrations of native Bcl-xL in C2C12 cell extract, L929 cell extract based on a 300 μg/mL extract load, and PC-12 cell extract samples based on a 150 μg/mL extract load.

The concentrations of Bcl-xL were measured in duplicate and interpolated from the Bcl-xL standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Bcl-xL concentration was determined to be 2.176 ng/mL in C2C12 cell extract, 6.010 ng/mL in L929 cell extract, and 6.022 ng/mL in PC-12 cell extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat Bcl-xL ELISA Kit (AB227899)

Representative image of Mouse/Rat Bcl-xL ELISA Kit (ab227899)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Mouse/Rat Bcl-xL Capture Antibody

- 10X Mouse/Rat Bcl-xL Detector Antibody

- Mouse/Rat Bcl-xL Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Sample diluent 5BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat Bcl-xL ELISA Kit (AB227899)

Example of mouse/rat Bcl-xL standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Mouse, Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 28 pg/mL

Range

0.19 - 12 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse/Rat Bcl-xL ELISA Kit (ab227899) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Bcl-xL protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat Bcl-xL with 28 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "L929", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.1" }, { "reproducibilityType": "Intra", "sample": "L929", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" } ]

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Recovery

[ { "sample": "Cell culture extracts", "range": "81 - 86 %", "average": "= 83" }, { "sample": "Mouse brain extract", "range": "94 - 111 %", "average": "= 105" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab227899-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB227899-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB227899-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB227899-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB227899-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB227899-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB227899-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB227899-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse/Rat Bcl-xL Detector Antibody", "number":"AB227899-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB227899-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB227899-CMP13", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse/Rat Bcl-xL Capture Antibody", "number":"AB227899-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse/Rat Bcl-xL Lyophilized Recombinant Protein", "number":"AB227899-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bcl-XL also known as Bcl-xL or Bcl-Xl protein is a member of the Bcl-2 protein family playing an important role in regulating cell death. Bcl-XL inhibits apoptosis by binding to pro-apoptotic proteins preventing the release of cytochrome c from mitochondria. This protein has a molecular weight of approximately 30 kDa. Bcl-xL is widely expressed in tissues notably in the brain endothelial cells and hematopoietic tissues where it contributes to cell survival.

Biological function summary

This protein acts as an anti-apoptotic molecule within the mitochondria and is part of the Bcl-2 family complex. Bcl-XL modulates the balance between pro-apoptotic and anti-apoptotic signals impacting cell fate. By binding and sequestering pro-apoptotic members such as Bax and Bak Bcl-XL prevents mitochondrial outer membrane permeabilization important for avoiding programmed cell death. This function not only supports cellular longevity but also understanding cancer cell survival.

Pathways

The Bcl-XL protein holds a significant position in the apoptotic signaling pathways. It is particularly involved in the intrinsic pathway where its interactions with mitochondrial proteins Bax and Bak determine cell survival or death. It is interconnected with the PI3K/AKT pathway where AKT kinase activity can upregulate Bcl-XL expression demonstrating how survival signals are transmitted. These interactions highlight its pivotal role in balancing life and death at the cellular level.

Bcl-XL's overexpression often connects to the progression of cancers and resistance to chemotherapy. By inhibiting programmed cell death Bcl-XL allows cancer cells to evade traditional treatments. Furthermore research implicates Bcl-XL in neurodegenerative disorders where its interaction with Bcl-2 proteins disrupts normal apoptotic processes contributing to cellular dysfunction and disease. Understanding Bcl-XL's disease-related roles furthers the development of targeted therapies combating its protective effects in pathological contexts.

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Product protocols

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Target data

Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.. Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles (By similarity). May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (By similarity).. Isoform Bcl-X(S) promotes apoptosis.

See full target information Bcl2l1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed39405093

2024

A new potential strategy for cutaneous squamous cell carcinoma treatment by generating serum-based antibodies from tumor-exposed mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Liu

Folia histochemica et cytobiologica 59:95-107 PubMed33876830

2021

Does repeated gold-nanoparticles administration affect pars distalis hormonal and folliculo-stellate cells in adult male albino rats?

Applications

Unspecified application

Species

Unspecified reactive species

Abeer Ibraheem Omar,Samaa Samir Kamar

Pharmaceuticals (Basel, Switzerland) 14: PubMed33799790

2021

Tioconazole and Chloroquine Act Synergistically to Combat Doxorubicin-Induced Toxicity via Inactivation of PI3K/AKT/mTOR Signaling Mediated ROS-Dependent Apoptosis and Autophagic Flux Inhibition in MCF-7 Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Afnan H El-Gowily,Samah A Loutfy,Ehab M M Ali,Tarek M Mohamed,Mohammed A Mansour

Frontiers in pharmacology 9:347 PubMed29695964

2018

Root Extract of Ameliorates DSS-Induced Ulcerative Colitis by Affecting NF-kappaB Signaling Pathway in a Mouse Model via Synergistic Effects of Polydatin, Resveratrol, and Emodin.

Applications

Unspecified application

Species

Unspecified reactive species

Baohai Liu,Shuangdi Li,Xiaodan Sui,Lianyi Guo,Xingmei Liu,Hongmei Li,Leming Gao,Shusheng Cai,Yanrong Li,Tingting Wang,Xuehua Piao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse/Rat Bcl-xL ELISA Kit

Sandwich ELISA - Mouse/Rat Bcl-xL ELISA Kit (AB227899)

Sandwich ELISA - Mouse/Rat Bcl-xL ELISA Kit (AB227899)

Sandwich ELISA - Mouse/Rat Bcl-xL ELISA Kit (AB227899)

Sandwich ELISA - Mouse/Rat Bcl-xL ELISA Kit (AB227899)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Complementary Products

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise