Mouse/Rat Cytochrome C ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse/Rat Cytochrome C with a sensitivity of 44 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
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- Biology 11:2022Selenium Nanoparticles with Prodigiosin Rescue Hippocampal Damage Associated with Epileptic Seizures Induced by Pentylenetetrazole in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaif E Al Omairi,Ashraf Albrakati,Khalaf F Alsharif,Abdulraheem S Almalki,Walaa Alsanie,Zakaria Y Abd Elmageed,Dalia Zaafar,Maha S Lokman,Amira A Bauomy,Saied K Belal,Mohamed M Abdel-Daim,Ahmed E Abdel Moneim,Hussain Alyami,Rami B KassabPubMed 35336729
- Biomedicines 10:2022Cytoprotective Effect of Idebenone through Modulation of the Intrinsic Mitochondrial Pathway of Apoptosis in Human Retinal Pigment Epithelial Cells Exposed to Oxidative Stress Induced by Hydrogen Peroxide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Elisabetta Clementi,Michela Pizzoferrato,Giada Bianchetti,Anna Brancato,Beatrice Sampaolese,Giuseppe Maulucci,Giuseppe TringaliPubMed 35203712
- The Prostate 81:1135-11482021SIRT3 affects mitochondrial metabolic reprogramming via the AMPK-PGC-1α axis in the development of benign prostatic hyperplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongzhi Li,Qian Wang,Jingyu Li,Benkang Shi,Yili Liu,Ping WangPubMed 34411320
- Molecular medicine reports 22:4254-42642020Hyperbaric oxygen protects against myocardial ischemia‑reperfusion injury through inhibiting mitochondria dysfunction and autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWan Chen et. al.PubMed 32901878
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse, Rat
- Range
- 487.4 - 14000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 44 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
Alternative names
Cycs
What's included?
Recommended products
Mouse/Rat Cytochrome C ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse/Rat Cytochrome C with a sensitivity of 44 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse, Rat
- Range
- 487.4 - 14000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 44 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample NIH 3T3 Extract | n 5 | mean - | SD - | C.V. 2.7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample NIH 3T3 Extract | n 3 | mean - | SD - | C.V. 5.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Tissue Extracts | Average % = 108 | Range 106 - 112 % |
Sample type Cell culture media | Average % = 111 | Range 106 - 116 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse/Rat Cytochrome C ELISA Kit (ab210575) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cytochrome C protein in tissue extracts, cell culture media, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat Cytochrome C with 44 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Cytochrome C is 11 kDa mitochondrial intermembrane space electron carrier protein. The oxidized form of the cytochrome C heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome C then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.Cytochrome C also plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial outer membrane permeability resulting in release of cytochrome C into the cytosol. Binding of cytochrome C to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytochrome C also referred to as "cytochrome c" or "Cyt C" is a small protein with a molecular weight of approximately 12 kDa. It is a component of the electron transport chain playing an essential mechanical role in cellular respiration. Cytochrome C is expressed in the mitochondria of eukaryotic cells where it functions as a heme protein. The mass of this protein allows efficient electron transfer between Complex III (cytochrome bc1 complex) and Complex IV (cytochrome c oxidase) contributing to ATP synthesis.
- Biological function summary
Cytochrome c contributes to the process of oxidative phosphorylation within mitochondria. It is not only an important electron carrier but it also plays a significant role in apoptosis. In the event of cellular stress cytochrome c releases into the cytosol and forms part of the apoptosome complex. This apoptosome activation leads to the caspase cascade driving programmed cell death. Therefore cytochrome C serves as both a mediator of energy production and a regulator of apoptosis.
- Pathways
Cytochrome c is integral to the mitochondrial electron transport chain and apoptotic pathways. It interacts with proteins in these pathways such as cytochrome c oxidase in oxidative phosphorylation and Apaf-1 during apoptosis. The electron transport chain's function is dependent on the efficient transfer of electrons facilitated by cytochrome c ensuring ATP production in cellular respiration.
- Associated diseases and disorders
Cytochrome c links to conditions like cancer and neurodegenerative diseases. In cancer its role in the apoptotic pathway often emerges as cancer cells can develop mechanisms to evade apoptosis impacting cytochrome c release. For neurodegenerative diseases disruptions in mitochondrial function and cytochrome c's release can facilitate cell death implicating its role in conditions like Parkinson's disease. The interaction of cytochrome c with proteins such as p53 in response to cellular stress further highlights its involvement in disease pathogenesis.
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5 product images
Sandwich ELISA - Mouse/Rat Cytochrome C ELISA Kit (ab210575)
Mouse/Rat Cytochrome C standard curve comparison data.
Standard curve comparison between mouse/rat Cytochrome C SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 10-fold increase in sensitivity.
Sandwich ELISA - Mouse/Rat Cytochrome C ELISA Kit (ab210575)
Example of mouse/rat Cytochrome C standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse/Rat Cytochrome C ELISA Kit (ab210575)
Interpolated concentrations of native Cytochrome C in mouse heart tissue extract samples based on 15 µg/mL extract load and in rat heart tissue extract samples based on 7 µg/mL extract load.
The concentrations of Cytochrome C were measured in duplicate and interpolated from the Cytochrome C standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytochrome C concentration was determined to be 8,822 pg/mL in mouse heart extract and 4,109 pg/mL in rat heart extract samples.
Sandwich ELISA - Mouse/Rat Cytochrome C ELISA Kit (ab210575)
Interpolated concentrations of native Cytochrome C in mouse NIH/3T3 cell extract samples based on 65 µg/mL extract load, and mouse C2C12 and rat C6 cell extract samples based on 100 µg/mL extract.
The concentrations of Cytochrome C were measured in duplicate and interpolated from the Cytochrome C standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytochrome C concentration was determined to be 10,516 pg/mL in NIH/3T3 extract, 11,096 pg/mL in C2C12 extract, and 13,297 pg/mL in C6 extract.
Sandwich ELISA - Mouse/Rat Cytochrome C ELISA Kit (ab210575)
Interpolated concentrations of native Cytochrome C in mouse and rat extract samples.
The concentrations of Cytochrome C were measured in three different dilutions in duplicate and interpolated from the Cytochrome C standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng of Cytochrome C per mg of extract (mean +/-SD, n=3). Cytochrome C concentration was determined to be 601 ng/mg in mouse heart, 622 ng/mg in rat heart, 166 ng/mg in mouse NIH/3T3 cell, 105 ng/mg in mouse C2C12 cell and 136 ng/mg in rat C6 cell extract samples.
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