AB210575

Mouse/Rat Cytochrome C ELISA Kit

Recombinant
SimpleStep
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(6 Publications)

Mouse/Rat Cytochrome C ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse/Rat Cytochrome C with a sensitivity of 44 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

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Sandwich ELISA - Mouse/Rat Cytochrome C ELISA Kit (AB210575)

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat Cytochrome C ELISA Kit (AB210575)

Interpolated concentrations of native Cytochrome C in mouse NIH/3T3 cell extract samples based on 65 µg/mL extract load, and mouse C2C12 and rat C6 cell extract samples based on 100 µg/mL extract.

The concentrations of Cytochrome C were measured in duplicate and interpolated from the Cytochrome C standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytochrome C concentration was determined to be 10,516 pg/mL in NIH/3T3 extract, 11,096 pg/mL in C2C12 extract, and 13,297 pg/mL in C6 extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat Cytochrome C ELISA Kit (AB210575)

Interpolated concentrations of native Cytochrome C in mouse and rat extract samples.

The concentrations of Cytochrome C were measured in three different dilutions in duplicate and interpolated from the Cytochrome C standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng of Cytochrome C per mg of extract (mean +/-SD, n=3). Cytochrome C concentration was determined to be 601 ng/mg in mouse heart, 622 ng/mg in rat heart, 166 ng/mg in mouse NIH/3T3 cell, 105 ng/mg in mouse C2C12 cell and 136 ng/mg in rat C6 cell extract samples.

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat Cytochrome C ELISA Kit (AB210575)

Interpolated concentrations of native Cytochrome C in mouse heart tissue extract samples based on 15 µg/mL extract load and in rat heart tissue extract samples based on 7 µg/mL extract load.

The concentrations of Cytochrome C were measured in duplicate and interpolated from the Cytochrome C standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytochrome C concentration was determined to be 8,822 pg/mL in mouse heart extract and 4,109 pg/mL in rat heart extract samples.

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat Cytochrome C ELISA Kit (AB210575)

Example of mouse/rat Cytochrome C standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Mouse/Rat Cytochrome C ELISA Kit (AB210575)

Mouse/Rat Cytochrome C standard curve comparison data.

Standard curve comparison between mouse/rat Cytochrome C SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 10-fold increase in sensitivity.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Mouse, Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 44 pg/mL

Range

487.4 - 14000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse/Rat Cytochrome C ELISA Kit (ab210575) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cytochrome C protein in tissue extracts, cell culture media, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat Cytochrome C with 44 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Cytochrome C is 11 kDa mitochondrial intermembrane space electron carrier protein. The oxidized form of the cytochrome C heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome C then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.Cytochrome C also plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial outer membrane permeability resulting in release of cytochrome C into the cytosol. Binding of cytochrome C to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "NIH 3T3 Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" }, { "reproducibilityType": "Intra", "sample": "NIH 3T3 Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": "106 - 112 %", "average": "= 108" }, { "sample": "Cell culture media", "range": "106 - 116 %", "average": "= 111" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome C also referred to as "cytochrome c" or "Cyt C" is a small protein with a molecular weight of approximately 12 kDa. It is a component of the electron transport chain playing an essential mechanical role in cellular respiration. Cytochrome C is expressed in the mitochondria of eukaryotic cells where it functions as a heme protein. The mass of this protein allows efficient electron transfer between Complex III (cytochrome bc1 complex) and Complex IV (cytochrome c oxidase) contributing to ATP synthesis.

Biological function summary

Cytochrome c contributes to the process of oxidative phosphorylation within mitochondria. It is not only an important electron carrier but it also plays a significant role in apoptosis. In the event of cellular stress cytochrome c releases into the cytosol and forms part of the apoptosome complex. This apoptosome activation leads to the caspase cascade driving programmed cell death. Therefore cytochrome C serves as both a mediator of energy production and a regulator of apoptosis.

Pathways

Cytochrome c is integral to the mitochondrial electron transport chain and apoptotic pathways. It interacts with proteins in these pathways such as cytochrome c oxidase in oxidative phosphorylation and Apaf-1 during apoptosis. The electron transport chain's function is dependent on the efficient transfer of electrons facilitated by cytochrome c ensuring ATP production in cellular respiration.

Cytochrome c links to conditions like cancer and neurodegenerative diseases. In cancer its role in the apoptotic pathway often emerges as cancer cells can develop mechanisms to evade apoptosis impacting cytochrome c release. For neurodegenerative diseases disruptions in mitochondrial function and cytochrome c's release can facilitate cell death implicating its role in conditions like Parkinson's disease. The interaction of cytochrome c with proteins such as p53 in response to cellular stress further highlights its involvement in disease pathogenesis.

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Product protocols

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Target data

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

See full target information Cycs

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical research 39:340-355 PubMed39618181

2024

Hepatic SIRT6 protects against cholestatic liver disease primarily inhibiting bile acid synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Zhang,Jiahui Wang,Luyao Yang,Yuyun Shao,Hongjun Peng,Longfeng Jiang,Liang Sheng

Frontiers in pharmacology 15:1398630 PubMed39611167

2024

High-density lipoprotein protects normotensive and hypertensive rats against ischemia-reperfusion injury through differential regulation of mTORC1 and mTORC2 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Reham Al-Othman,Aishah Al-Jarallah,Fawzi Babiker

Biology 11: PubMed35336729

2022

Selenium Nanoparticles with Prodigiosin Rescue Hippocampal Damage Associated with Epileptic Seizures Induced by Pentylenetetrazole in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Naif E Al Omairi,Ashraf Albrakati,Khalaf F Alsharif,Abdulraheem S Almalki,Walaa Alsanie,Zakaria Y Abd Elmageed,Dalia Zaafar,Maha S Lokman,Amira A Bauomy,Saied K Belal,Mohamed M Abdel-Daim,Ahmed E Abdel Moneim,Hussain Alyami,Rami B Kassab

Biomedicines 10: PubMed35203712

2022

Cytoprotective Effect of Idebenone through Modulation of the Intrinsic Mitochondrial Pathway of Apoptosis in Human Retinal Pigment Epithelial Cells Exposed to Oxidative Stress Induced by Hydrogen Peroxide.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Elisabetta Clementi,Michela Pizzoferrato,Giada Bianchetti,Anna Brancato,Beatrice Sampaolese,Giuseppe Maulucci,Giuseppe Tringali

The Prostate 81:1135-1148 PubMed34411320

2021

SIRT3 affects mitochondrial metabolic reprogramming via the AMPK-PGC-1α axis in the development of benign prostatic hyperplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Yongzhi Li,Qian Wang,Jingyu Li,Benkang Shi,Yili Liu,Ping Wang

Molecular medicine reports 22:4254-4264 PubMed32901878

2020

Hyperbaric oxygen protects against myocardial ischemia‑reperfusion injury through inhibiting mitochondria dysfunction and autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Wan Chen,Liwen Lv,Zhihuan Nong,Xiaoyu Chen,Xiaorong Pan,Chunxia Chen

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse/Rat Cytochrome C ELISA Kit

Sandwich ELISA - Mouse/Rat Cytochrome C ELISA Kit (AB210575)

Sandwich ELISA - Mouse/Rat Cytochrome C ELISA Kit (AB210575)

Sandwich ELISA - Mouse/Rat Cytochrome C ELISA Kit (AB210575)

Sandwich ELISA - Mouse/Rat Cytochrome C ELISA Kit (AB210575)

Sandwich ELISA - Mouse/Rat Cytochrome C ELISA Kit (AB210575)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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