AB223587

Mouse/Rat FGF1 ELISA Kit

Recombinant
SimpleStep
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(3 Publications)

Mouse/Rat FGF1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse/Rat FGF1 with a sensitivity of 2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Fgf-1, Fgfa, Fgf1, Fibroblast growth factor 1, FGF-1, Acidic fibroblast growth factor, Heparin-binding growth factor 1, aFGF, HBGF-1

8 Images

Sandwich ELISA - Mouse/Rat FGF1 ELISA Kit (AB223587)

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat FGF1 ELISA Kit (AB223587)

Interpolated concentrations of native mouse/rat FGF1 in mouse and rat brain tissue extract based on a 31 or 63 μg/mL extract load, respectively. The concentrations of FGF1 were measured in duplicate and interpolated from the FGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF1 concentration was determined to be 697 pg/mL in mouse brain tissue extract and 796 pg/mL in rat brain tissue extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat FGF1 ELISA Kit (AB223587)

Interpolated concentrations of spiked mouse/rat FGF1 in mouse serum, plasma and cell culture supernatant samples.

The concentrations of FGF1 were measured in duplicates, interpolated from the FGF1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 25% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat FGF1 ELISA Kit (AB223587)

Example of mouse/rat FGF1 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat FGF1 ELISA Kit (AB223587)

Example of mouse/rat FGF1 standard curve in Sample Diluent 25BS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat FGF1 ELISA Kit (AB223587)

Example of mouse/rat FGF1 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat FGF1 ELISA Kit (AB223587)

Human FGF1 active protein (hFGF1) was added at the indicated concentrations to 500 pg/ml mouse FGF1 (mFGF1) to test for Interference.

Only 14% interference was observed with the highest dose of hFGF1.

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat FGF1 ELISA Kit (AB223587)

Interpolated concentrations of spiked mouse/rat FGF1 in rat serum and plasma samples.

The concentrations of FGF1 were measured in duplicates, interpolated from the FGF1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 50%, and RAW 264.7 supernatant 95%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat FGF1 ELISA Kit (AB223587)

Interpolated concentrations of spiked FGF1 in mouse and rat urine.

The concentrations of FGF1 were measured in duplicate and interpolated from the FGF1 standard curve and corrected for sample dilution. Undiluted samples are as follows : mouse urine 25%, rat urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Urine, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse, Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 2 pg/mL

Range

16 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse/Rat FGF1 ELISA Kit (ab223587) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGF1 protein in cit plasma, edta plasma, serum, tissue lysate, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat FGF1 with NULL NULL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

FGF1, also known as Fibroblast Growth Factor 1 or FGF acidic, is an important activator for several signaling cascades with roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. It functions as potent mitogen in vitro. In the presence of heparin, FGF1 binds in a 1:2 stoichiometry with FGF1 receptor protein, causing the autophosphorylation and activation of the receptor. This complex then binds to integrin and recruits PTPN11 to begin FGF1 signaling. Because of its possible role in organ development and in tumor growth, FGF1 is an important subject of study for the treatment of diabetes and the diagnosis and treatment of cancer. Mouse and rat FGF1 are identical on an amino acid level.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Brain Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" }, { "reproducibilityType": "Intra", "sample": "Brain Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]

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Recovery

[ { "sample": "Serum", "range": "111 - 119 %", "average": "= 116" }, { "sample": "EDTA Plasma", "range": "102 - 109 %", "average": "= 105" }, { "sample": "Tissue Lysate", "range": "105 - 126 %", "average": "= 118" }, { "sample": "Urine", "range": "95 - 135 %", "average": "= 111" }, { "sample": "Citrate plasma", "range": "111 - 125 %", "average": "= 119" }, { "sample": "Cell culture media", "range": "108 - 125 %", "average": "= 117" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab223587-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB223587-CMP14", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse/Rat FGF1 Capture Antibody", "number":"AB223587-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse/Rat FGF1 Lyophilized Recombinant Protein", "number":"AB223587-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB223587-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB223587-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB223587-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BS", "number":"AB223587-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB223587-CMP13", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB223587-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB223587-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB223587-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB223587-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse/Rat FGF1 Detector Antibody", "number":"AB223587-CMP10", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF1 also known as acidic fibroblast growth factor (aFGF) is a member of the fibroblast growth factor family. This protein has a molecular mass of approximately 17 kDa. FGF1 is expressed in a variety of tissues such as the brain liver and heart and plays an essential role in cell growth development and repair. The FGF1 protein is sometimes studied using techniques such as FGF1 ELISA which helps in quantifying and understanding its expression levels and activity in different contexts.

Biological function summary

FGF1 is involved in numerous cellular processes beyond simple cell proliferation. It binds to fibroblast growth factor receptors (FGFRs) on the cell surface initiating a cascade of downstream signaling events. This protein does not act alone; it is often a part of larger protein complexes that modulate cellular processes such as angiogenesis and wound healing. FGF1 is also implicated in nerve cell differentiation and survival through FGFR-mediated pathways.

Pathways

The FGF1 protein is integrally involved in the MAPK/ERK signaling pathway a critical pathway that regulates cell division growth and differentiation. Through this pathway FGF1 interacts with various proteins including Ras Raf and ERK to propagate the signal necessary for mitogenic and survival responses. FGF1 is also a player in the phosphoinositide 3-kinase (PI3K) pathway which helps control cellular responses to stress and metabolic regulation showing its role in cellular homeostasis.

FGF1 is associated with conditions such as cancer and cardiovascular diseases. The deregulation of FGF1 expression or its signaling pathways can lead to tumor development and progression with FGF1 often interacting with other proteins like the basic fibroblast growth factor (bFGF) in these contexts. In cardiovascular diseases altered FGF1 signaling might contribute to atherosclerosis and cardiac repair processes where its interaction with other growth factors like vascular endothelial growth factor (VEGF) is significant.

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Product protocols

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Target data

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV : ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis.

See full target information Fgf1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:2099 PubMed37055407

2023

High ploidy large cytoplasmic megakaryocytes are hematopoietic stem cells regulators and essential for platelet production.

Applications

Unspecified application

Species

Unspecified reactive species

Shen Y Heazlewood,Tanveer Ahmad,Benjamin Cao,Huimin Cao,Melanie Domingues,Xuan Sun,Chad K Heazlewood,Songhui Li,Brenda Williams,Madeline Fulton,Jacinta F White,Tom Nebl,Christian M Nefzger,Jose M Polo,Benjamin T Kile,Felix Kraus,Michael T Ryan,Yu B Sun,Peter F M Choong,Sarah L Ellis,Minna-Liisa Anko,Susan K Nilsson

Frontiers in neurology 13:986377 PubMed36188412

2022

study of decellularized rat tissues for nerve regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ye,Andong He,Miaoben Wu,Xiaodong Qiu,Zhiwu Chen,Jun Yin,Qinghua Song,Yi Huang,Kailei Xu,Yuye Huang,Peng Wei

Bioscience reports 39: PubMed31138757

2019

Low concentration of rutin treatment might alleviate the cardiotoxicity effect of pirarubicin on cardiomyocytes via activation of PI3K/AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Fei,Yi Sun,Yuyin Duan,Jianming Xia,Songhua Yu,Peigang Ouyang,Teng Wang,Guimin Zhang

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse/Rat FGF1 ELISA Kit

Sandwich ELISA - Mouse/Rat FGF1 ELISA Kit (AB223587)

Sandwich ELISA - Mouse/Rat FGF1 ELISA Kit (AB223587)

Sandwich ELISA - Mouse/Rat FGF1 ELISA Kit (AB223587)

Sandwich ELISA - Mouse/Rat FGF1 ELISA Kit (AB223587)

Sandwich ELISA - Mouse/Rat FGF1 ELISA Kit (AB223587)

Sandwich ELISA - Mouse/Rat FGF1 ELISA Kit (AB223587)

Sandwich ELISA - Mouse/Rat FGF1 ELISA Kit (AB223587)

Sandwich ELISA - Mouse/Rat FGF1 ELISA Kit (AB223587)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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