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AB285344

Mouse/rat Prolactin ELISA Kit

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Mouse/rat Prolactin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse/rat Prolactin in Mouse, Rat in Biofluids samples.

View Alternative Names

Prolactin, PRL, Prl

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Mouse, Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 0.2 ng/mL

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This Mouse/rat Prolactin kit (ab285344, K4688) is a solid phase sandwich ELISA Kit. The samples and biotinylated anti-prolactin antibodies are added to the wells coated with polyclonal antibody to prolactin. Prolactin in samples binds to the anti-prolactin polyclonal antibody on the well and the biotinylated anti-prolactin antibody binds to prolactin. Unbound protein and biotin conjugate are washed off by wash buffer. Horseradish peroxidase conjugated streptavidin is added to each wells and the streptavidin is allowed to bind to the biotin in the complex. Unbound enzyme conjugate is washed off. Upon the addition of the substrate, the intensity of color is proportional to the concentration of prolactin in the samples. A standard curve is prepared relating color intensity to the concentration of the prolactin.
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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" } ]
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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285344-96Test", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Streptavidin Enzyme Conjugate", "number":"AB285344-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate", "number":"AB285344-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Concentrate", "number":"AB285344-CMP04", "productcode":"" }, { "size":"6 x 1 Vial", "name":"Prolactin Standard", "number":"AB285344-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Prolactin Mab pre-coated Microplate", "number":"AB285344-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Biotinylated Antibody Reagent", "number":"AB285344-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB285344-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prolactin also known as PRL is a protein hormone with a molecular mass of approximately 23 kDa. It is primarily produced in the anterior pituitary gland but is also found in other tissues such as the placenta mammary glands and immune cells. Mechanically Prolactin functions as a peptide hormone and exerts its effects by binding to the Prolactin receptor a member of the cytokine receptor superfamily. This binding activates the Jak-STAT signaling pathway which is important for mediating the hormone's effects in target cells.
Biological function summary

The functions of this hormone extend beyond its well-known role in lactation. It helps stimulate milk production in the mammary glands by promoting the proliferation and differentiation of mammary epithelial cells. Additionally Prolactin influences reproductive functions metabolism and immune regulation. It is not part of a larger protein complex but interacts with various proteins to perform its functions. Notably it modulates the immune response contributing to tolerance during pregnancy and affecting the balance between pro-inflammatory and anti-inflammatory cytokines.

Pathways

Prolactin plays important roles in the Jak-STAT and MAPK signaling pathways. In these pathways it shares interactions with related proteins such as Growth Hormone and Prolactin Receptor aiding in cell growth differentiation and metabolism regulation. Prolactin's involvement in these pathways highlights its central role in physiological processes linking various hormonal signals to regulate body functions efficiently.

Prolactin's imbalance can lead to conditions such as hyperprolactinemia and prolactinomas both characterized by excessive levels of this hormone in the blood. These conditions are often related to alterations in the pituitary gland function. Prolactin interacts closely with Dopamine a neurotransmitter that inhibits its secretion influencing both its production and release. Dysregulation of this interaction may contribute to the development of these disorders impacting endocrine health and overall well-being.
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Product protocols

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Target data

Prolactin acts primarily on the mammary gland by promoting lactation.
See full target information Prl
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websiteProtocolBooklet
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Complementary Products

Assay Kits

AB238259

Rat Prolactin Receptor / PRL-R ELISA Kit

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Sandwich ELISA - Rat Prolactin Receptor / PRL-R ELISA Kit (AB238259)

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Primary Antibodies

AB188229

Anti-Prolactin/PRL antibody [EPR18018-31]

RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (AB188229)

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Assay Kits

AB263880

Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone)

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ELISA - Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (AB263880)

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Proteins & Peptides

AB243239

Recombinant mouse Prolactin/PRL protein (Active)

Bioactive

SDS-PAGE - Recombinant mouse Prolactin/PRL protein (Active) (AB243239)

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Product promise

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