Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
Featured events
Make new connections at our global events.
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Product Insider Program
Be the first to know about our latest product launches - and unlock exclusive offers and discounts.
Mouse/Rat Thyroxine (T4) ELISA Kit is a Competitive ELISA kit for the measurement of Mouse/Rat Thyroxine (T4) in Mouse, Rat in Plasma, Serum samples.
Colorimetric
Plasma, Serum
Competitive
Mouse, Rat
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Mouse/Rat Thyroxine (T4) ELISA Kit is a Competitive ELISA kit for the measurement of Mouse/Rat Thyroxine (T4) in Mouse, Rat in Plasma, Serum samples.
Colorimetric
Plasma, Serum
Competitive
Mouse, Rat
Pre-coated microplate (12 x 8 well strips)
Blue Ice
+4°C
+4°C
+4°C
T4 is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T4 is decreased in hypothyroid patients and is increased in hyperthyroid patients. The mouse/rat thyroxine (T4) kit (ab285258, K7421) is a solid phase competitive ELISA Kit. The samples, and T4 enzyme conjugate are added to the wells coated with anti-T4 polyclonal antibody. T4 in the sample competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound T4 and T4 enzyme conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T4 in the samples. A standard curve is prepared relating color intensity to the concentration of the T4.
Thyroxine acts on tissues throughout the body affecting growth development and metabolism. It is not part of a simple structure but rather influences multiple aspects of endocrinological systems. On a cellular level T4 enters target cells and converts to its active form triiodothyronine (T3) which then binds to thyroid hormone receptors inside the nucleus. This binding alters gene transcription modulating metabolic activity within those cells. Although T4 itself does not form complexes its metabolic conversion and subsequent gene regulatory functions play complex roles in cellular processes.
Thyroxine also known as T4 is a hormone that originates from the thyroid gland. It plays a mechanical role in regulating the basal metabolic rate by controlling the rate of oxygen use by cells. Its molecular weight is approximately 776.87 Da. T4 primarily circulates in the bloodstream bound to carrier proteins such as thyroxine-binding globulin transthyretin and albumin. The thyroid gland synthesizes and releases T4 in response to stimulation by thyroid-stimulating hormone (TSH). This hormone is important for maintaining metabolic processes and occurs both in human and mouse models demonstrating its cross-species significance.
Thyroxine is integral to the hypothalamic-pituitary-thyroid (HPT) axis and metabolic pathway. The HPT axis involves TSH which stimulates the release of T4 forming a feedback loop essential for maintaining homeostasis. T4 impacts various metabolic pathways directly influencing proteins such as deiodinases which convert T4 into the more active T3. Additionally T4 affects pathways involving energy production thermoregulation and lipid metabolism showcasing its widespread importance in physiological processes.
Thyroxine imbalance is directly linked to conditions like hypothyroidism and hyperthyroidism. In hypothyroidism there's insufficient T4 leading to symptoms such as fatigue weight gain and depression. Conversely hyperthyroidism involves excess T4 causing symptoms like weight loss increased heart rate and anxiety. Proteins such as TSH and thyroglobulin are significantly affected by these conditions as their levels can indicate thyroid gland dysfunction. Understanding T4's role provides insights into therapeutic interventions targeting thyroid-related disorders highlighting the importance of effective monitoring through assays such as TSH ELISA and other diagnostic tools.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com