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AB285258

Mouse/Rat Thyroxine (T4) ELISA Kit

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(1 Publication)

Mouse/Rat Thyroxine (T4) ELISA Kit is a competitive ELISA used to quantify Mouse/Rat Thyroxine (T4) with a sensitivity of 0.467 µg/dL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Validated on a number of sample types including serum and plasma

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Mouse, Rat

Assay type

Competitive

Results type

Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse/Rat Thyroxine (T4) ELISA Kit ab285258 is a competitive ELISA kit to measure Mouse/Rat Thyroxine (T4) in Serum and Plasma with a sensitivity of 55.8 pg/mL.

How the assay works

Mouse/Rat Thyroxine (T4) ELISA Kit ab285258 is a competitive ELISA. The samples and the diluted T4 enzyme conjugate are added to the wells coated with anti-T4 polyclonal antibody. T4 in the serum competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound T4 and T4 enzyme conjugate is washed off by wash buffer during a wash step. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T4 in the samples. A standard curve is generated relating color intensity to the concentration of the T4.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.

Mouse/Rat Thyroxine (T4) ELISA Kit ab285258 protocol summary:

1. Add samples and standards to wells.
2. Add diluted T4 enzyme conjugate to all wells. Incubate at room temperature.
3. Wash each well and add TMB Solution. Incubate at room temperature.
4. Add Stop Solution to each well. Read immediately.
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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285258-96Test", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Assay Diluent", "number":"AB285258-CMP01", "productcode":"" }, { "size":"2 x 0.25 mL", "name":"Controls", "number":"AB285258-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB285258-CMP02", "productcode":"" }, { "size":"7 x 0.25 mL", "name":"T4 Standard", "number":"AB285258-CMP06", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"T4 Enzyme Conjugate", "number":"AB285258-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Concentrate", "number":"AB285258-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"T4 Ab pre-coated Microplate", "number":"AB285258-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate", "number":"AB285258-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thyroxine also known as T4 is a hormone that originates from the thyroid gland. It plays a mechanical role in regulating the basal metabolic rate by controlling the rate of oxygen use by cells. Its molecular weight is approximately 776.87 Da. T4 primarily circulates in the bloodstream bound to carrier proteins such as thyroxine-binding globulin transthyretin and albumin. The thyroid gland synthesizes and releases T4 in response to stimulation by thyroid-stimulating hormone (TSH). This hormone is important for maintaining metabolic processes and occurs both in human and mouse models demonstrating its cross-species significance.
Biological function summary

Thyroxine acts on tissues throughout the body affecting growth development and metabolism. It is not part of a simple structure but rather influences multiple aspects of endocrinological systems. On a cellular level T4 enters target cells and converts to its active form triiodothyronine (T3) which then binds to thyroid hormone receptors inside the nucleus. This binding alters gene transcription modulating metabolic activity within those cells. Although T4 itself does not form complexes its metabolic conversion and subsequent gene regulatory functions play complex roles in cellular processes.

Pathways

Thyroxine is integral to the hypothalamic-pituitary-thyroid (HPT) axis and metabolic pathway. The HPT axis involves TSH which stimulates the release of T4 forming a feedback loop essential for maintaining homeostasis. T4 impacts various metabolic pathways directly influencing proteins such as deiodinases which convert T4 into the more active T3. Additionally T4 affects pathways involving energy production thermoregulation and lipid metabolism showcasing its widespread importance in physiological processes.

Thyroxine imbalance is directly linked to conditions like hypothyroidism and hyperthyroidism. In hypothyroidism there's insufficient T4 leading to symptoms such as fatigue weight gain and depression. Conversely hyperthyroidism involves excess T4 causing symptoms like weight loss increased heart rate and anxiety. Proteins such as TSH and thyroglobulin are significantly affected by these conditions as their levels can indicate thyroid gland dysfunction. Understanding T4's role provides insights into therapeutic interventions targeting thyroid-related disorders highlighting the importance of effective monitoring through assays such as TSH ELISA and other diagnostic tools.
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Product protocols

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Target data

See full target information DL-Thyroxine
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

European journal of medical research 30:459 PubMed40481542

2025

CBL-mediated AQP1 ubiquitination aggravates kidney-yang deficiency syndrome by promoting lipid metabolism dysregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yifei Wang,Jun Yuan,Wenfang He,Nan Yang,Lanjun Fu,Juan Jin
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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