Skip to main content

Mouse/Rat Thyroxine (T4) ELISA Kit is a Competitive ELISA kit for the measurement of Mouse/Rat Thyroxine (T4) in Mouse, Rat in Plasma, Serum samples.

Be the first to review this product! Submit a review

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Assay type

Competitive

Reactive species

Mouse, Rat

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Application

cELISA

Reactivity

Reacts

Dilution info

-

Notes

-

What's included?

96 Test
Components
20X Wash Concentrate
1 x 25 mL
Assay Diluent
1 x 12 mL
Controls
2 x 0.5 mL
Stop Solution
1 x 12 mL
T4 Ab pre-coated Microplate
1 x 1 Unit
T4 Enzyme Conjugate
1 x 1.5 mL
T4 Standard
7 x 0.25 mL
TMB Substrate
1 x 12 mL

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Mouse/Rat Thyroxine (T4) ELISA Kit is a Competitive ELISA kit for the measurement of Mouse/Rat Thyroxine (T4) in Mouse, Rat in Plasma, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Assay type

Competitive

Reactive species

Mouse, Rat

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

T4 is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T4 is decreased in hypothyroid patients and is increased in hyperthyroid patients. The mouse/rat thyroxine (T4) kit (ab285258, K7421) is a solid phase competitive ELISA Kit. The samples, and T4 enzyme conjugate are added to the wells coated with anti-T4 polyclonal antibody. T4 in the sample competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound T4 and T4 enzyme conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T4 in the samples. A standard curve is prepared relating color intensity to the concentration of the T4.

Supplementary info

Biological function summary

Thyroxine acts on tissues throughout the body affecting growth development and metabolism. It is not part of a simple structure but rather influences multiple aspects of endocrinological systems. On a cellular level T4 enters target cells and converts to its active form triiodothyronine (T3) which then binds to thyroid hormone receptors inside the nucleus. This binding alters gene transcription modulating metabolic activity within those cells. Although T4 itself does not form complexes its metabolic conversion and subsequent gene regulatory functions play complex roles in cellular processes.

Activity summary

Thyroxine also known as T4 is a hormone that originates from the thyroid gland. It plays a mechanical role in regulating the basal metabolic rate by controlling the rate of oxygen use by cells. Its molecular weight is approximately 776.87 Da. T4 primarily circulates in the bloodstream bound to carrier proteins such as thyroxine-binding globulin transthyretin and albumin. The thyroid gland synthesizes and releases T4 in response to stimulation by thyroid-stimulating hormone (TSH). This hormone is important for maintaining metabolic processes and occurs both in human and mouse models demonstrating its cross-species significance.

Pathways

Thyroxine is integral to the hypothalamic-pituitary-thyroid (HPT) axis and metabolic pathway. The HPT axis involves TSH which stimulates the release of T4 forming a feedback loop essential for maintaining homeostasis. T4 impacts various metabolic pathways directly influencing proteins such as deiodinases which convert T4 into the more active T3. Additionally T4 affects pathways involving energy production thermoregulation and lipid metabolism showcasing its widespread importance in physiological processes.

Associated diseases and disorders

Thyroxine imbalance is directly linked to conditions like hypothyroidism and hyperthyroidism. In hypothyroidism there's insufficient T4 leading to symptoms such as fatigue weight gain and depression. Conversely hyperthyroidism involves excess T4 causing symptoms like weight loss increased heart rate and anxiety. Proteins such as TSH and thyroglobulin are significantly affected by these conditions as their levels can indicate thyroid gland dysfunction. Understanding T4's role provides insights into therapeutic interventions targeting thyroid-related disorders highlighting the importance of effective monitoring through assays such as TSH ELISA and other diagnostic tools.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

0 product images

    Downloads

    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com

    There was a problem

    We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com