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AB233615

Mouse/Rat TPA ELISA Kit

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Mouse/Rat TPA ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse/Rat TPA with a sensitivity of 4.98 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Tissue-type plasminogen activator, t-PA, t-plasminogen activator, tPA, Plat

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Sandwich ELISA - Mouse/Rat TPA ELISA Kit (AB233615)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat TPA ELISA Kit (AB233615)

Interpolated concentrations of native tPA in rat serum, plasma and urine samples.

The concentrations of tPA were measured in duplicates, interpolated from the tPA standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 12.5%, and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean tPA concentration in the neat samples was determined to be 1,657 pg/mL in serum, 5,687 pg/mL in plasma (citrate), and 388 pg/mL in urine.

Sandwich ELISA - Mouse/Rat TPA ELISA Kit (AB233615)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat TPA ELISA Kit (AB233615)

Interpolated concentrations of native tPA in mouse and rat serum, plasma and urine samples.

The concentrations of tPA were measured in duplicates, interpolated from the tPA standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (heparin) 12.5%, plasma (EDTA) 12.5%, plasma (citrate) 12.5%, and urine 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean tPA concentration in the neat samples was determined to be 540 pg/mL in serum, 2,567 pg/mL in plasma (heparin), 1,971 pg/mL in plasma (EDTA), 3,141 pg/mL in plasma (citrate), and 2,223 pg/mL in urine.

Sandwich ELISA - Mouse/Rat TPA ELISA Kit (AB233615)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat TPA ELISA Kit (AB233615)

Example of mouse and rat tPA standard curve in Sample Diluent NS.

The tPA standard curve was prepared as described in Section 10.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse, Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4.98 pg/mL

Range

11.72 - 750 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse/Rat TPA ELISA Kit (ab233615) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TPA protein in cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat TPA with 4.98 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

The standard is affinity purified.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.88" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.12" } ]
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Recovery

[ { "sample": "Serum", "range": "95 - 98 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "97 - 106 %", "average": "= 102" }, { "sample": "Urine", "range": "105 - 116 %", "average": "= 112" }, { "sample": "Heparin Plasma", "range": "97 - 101 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "99 - 108 %", "average": "= 104" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab233615-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB233615-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB233615-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB233615-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB233615-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB233615-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse/Rat tPA Detector Antibody", "number":"AB233615-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB233615-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB233615-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse/Rat tPA Capture Antibody", "number":"AB233615-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse/Rat tPA Lyophilized Recombinant Protein", "number":"AB233615-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Plasminogen Activator (tPA) also known as a plasminogen activator acts mechanically to convert plasminogen to plasmin a serine protease. This reaction occurs at the surface of a fibrin clot leading to clot degradation a process known as fibrinolysis. tPA has a molecular mass of approximately 70 kDa. It is mainly expressed in vascular endothelial cells and is released into the bloodstream in response to stimuli such as circulatory stasis or endothelial damage.
Biological function summary

TPA plays a critical role in thrombolysis by breaking down blood clots into their soluble components. It regulates plasminogen function by cleaving this zymogen to yield the active protease plasmin. This function makes tPA integral in maintaining hemostasis and it does not form a part of a larger protein complex. The activity and the regulation of tPA are important for preventing pathologic clotting which can lead to cardiovascular complications.

Pathways

TPA is central to the fibrinolytic pathway. This pathway facilitates the conversion of plasminogen to plasmin enabling clot resolution. In addition tPA interacts with other proteins such as urokinase plasminogen activator (uPA) and inhibitors like plasminogen activator inhibitor-1 (PAI-1). The balance between tPA and its inhibitors is important for the regulation of fibrinolytic activity impacting hemostatic and thrombotic events.

TPA connects closely with conditions like stroke and myocardial infarction due to its thrombolytic properties. In ischemic stroke excessive or insufficient tPA activity can disrupt normal blood flow leading to tissue damage. Additionally in myocardial infarction tPA's role in breaking down clots proves important for restoring coronary blood flow. It is also linked with proteins like fibrinogen as they serve as substrates in the clot degradation process and with PAI-1 which modulates its activity and influences disease progression.
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Product protocols

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Target data

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy (By similarity).
See full target information Plat
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Product promise

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