JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB285259

Mouse/Rat Triiodothyronine (T3) ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse/Rat Triiodothyronine (T3) ELISA Kit is a competitive ELISA designed to quantify Mouse/Rat Triiodothyronine (T3) with a sensitivity of 0.186 ng/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.5 - 1.5 ng/ml

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Rat, Mouse

Assay type

Competitive

Sensitivity

= 0.186 ng/mL

Range

0.5 - 1.5 ng/mL

Assay Platform

Microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Triiodothyronine (T3) is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T3 is decreased in hypothyroid patients and is increased in hyperthyroid patients. The mouse/rat Triiodothyronine (T3) kit (ab285259, K7422) is a solid phase competitive ELISA Kit. The samples, and T3 enzyme conjugate are added to the wells coated with anti-T3 polyclonal antibody. T3 in the sample competes with a T3 enzyme (HRP) conjugate for binding sites. Unbound T3 and T3 enzyme conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T3 in the samples. A standard curve is prepared relating color intensity to the concentration of the T3.
style
left-column

What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285259-96Test", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Assay Diluent", "number":"AB285259-CMP01" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB285259-CMP03" }, { "size":"1 x 1.5 mL", "name":"T3 Enzyme Conjugate", "number":"AB285259-CMP05" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB285259-CMP02" }, { "size":"7 x 1 Vial", "name":"T3 Standard", "number":"AB285259-CMP06" }, { "size":"1 x 1 Unit", "name":"T3 Ab pre-coated Microplate", "number":"AB285259-CMP04" }, { "size":"1 x 12 mL", "name":"TMB Substrate", "number":"AB285259-CMP07" }, { "size":"2 x 0.25 mL", "name":"T3 Controls", "number":"AB285259-CMP08" } ] }, "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285259-96Test", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB285259-CMP02", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"T3 Enzyme Conjugate", "number":"AB285259-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB285259-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Assay Diluent", "number":"AB285259-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate", "number":"AB285259-CMP07", "productcode":"" }, { "size":"7 x 1 Vial", "name":"T3 Standard", "number":"AB285259-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"T3 Ab pre-coated Microplate", "number":"AB285259-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Triiodothyronine (T3) also known as liothyronine is a thyroid hormone with a molecular mass of approximately 651.97 Da. It forms when enzymes called deiodinases especially in the liver and kidneys remove an iodine from thyroxine (T4). T3 is mainly expressed in cells of tissues with high metabolic activity including the heart liver and brain. In plasma T3 travels bound to proteins like thyroxine-binding globulin which helps maintain its stable concentration.
Biological function summary

T3 plays a central role in regulating metabolic processes and energy homeostasis. It acts on nearly every cell in the body influencing protein synthesis increasing basal metabolic rate and consequently affecting development growth and metabolism. T3 exerts its effects by binding to nuclear thyroid hormone receptors forming a hormone-receptor complex that regulates gene expression. This hormone engages in crosstalk with other signaling molecules linking it to broader metabolic networks.

Pathways

T3 integrates into the thyroid hormone signaling pathway influencing various metabolic and physiological functions. This pathway includes the conversion of T4 to T3 by iodothyronine deiodinases. T3 also interacts with the PI3K/Akt pathway an important signaling cascade involved in cellular survival and growth. Proteins like thyroid receptor alpha (TRα) and beta (TRβ) are vital in mediating the effects of T3 as they serve as binding sites for the hormone influencing the pathway's downstream function.

Alterations in T3 levels associate with conditions such as hyperthyroidism and hypothyroidism. Hyperthyroidism results from excess T3 leading to symptoms like increased heart rate and weight loss while hypothyroidism stems from T3 deficiency causing fatigue and weight gain. T3 plays a role in Graves’ disease an autoimmune disorder that can cause hyperthyroidism with thyroid-stimulating hormone receptor antibodies influencing the overproduction of the hormone. Dysregulation of T3 levels also impacts the cardiovascular system through its connection with proteins involved in heart function potentially contributing to heart disease.
style
left-column

Product protocols

style
left-column

Target data

See full target information Liothyronine
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB285258

Mouse/Rat Thyroxine (T4) ELISA Kit

  • 0
  • 0
Assay Kits

AB108685

Triiodothyronine (T3) ELISA Kit

Competitive ELISA - Triiodothyronine (T3) ELISA Kit (AB108685)

  • 4
  • 1
Assay Kits

AB272194

Human CG beta ELISA Kit (CGB3)

Recombinant|SimpleStep

Sandwich ELISA - Human CG beta ELISA Kit (CGB3) (AB272194)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com