Mouse Relaxin 1 ELISA Kit

Product details

The Mouse Relaxin 1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213885) is designed for the quantitative measurement of Mouse Relaxin 1 in cell culture supernatants, serum and plasma (heparin or EDTA ).

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for Relaxin 1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: R23-R54 (B) & E161-C185 (A)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Relaxin 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Relaxin 1 amount of sample captured in plate.

Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to mouse chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix (cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.