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AB193728

Mouse Renin 1 ELISA Kit

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(4 Publications)

Mouse Renin 1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Renin 1 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Atp6ip2, Atp6ap2, Renin receptor, ATPase H(+)-transporting lysosomal accessory protein 2, ATPase H(+)-transporting lysosomal-interacting protein 2, Renin/prorenin receptor

2 Images
Sandwich ELISA - Mouse Renin 1 ELISA Kit (AB193728)
  • sELISA

Unknown

Sandwich ELISA - Mouse Renin 1 ELISA Kit (AB193728)

Example of typical mouse Renin 1 standard curve using Assay Diluent A

Sandwich ELISA - Mouse Renin 1 ELISA Kit (AB193728)
  • sELISA

Unknown

Sandwich ELISA - Mouse Renin 1 ELISA Kit (AB193728)

Example of typical mouse Renin 1 standard curve using 1x Assay Diluent B

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 6 pg/mL

Range

6 - 50000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Renin 1 mouse ELISA kit (ab193728) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Renin 1 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse Renin 1 coated on a 96 well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Renin 1 present in the sample. The wells are washed and biotinylated anti-mouse Renin 1 antibody is added. After washing away unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted into the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color develops in proportion to the amount of Renin 1 bound in each well. Addition of the Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "87 - 132 %", "average": "= 107.3" }, { "sample": "Serum", "range": "112 - 145 %", "average": "= 130.5" }, { "sample": "Plasma", "range": "128 - 142 %", "average": "= 134.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193728-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193728-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193728-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Mouse Renin 1 detection antibody", "number":"AB193728-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB193728-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB193728-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"mouse Renin 1 Standards (lyophilized)", "number":"AB193728-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193728-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated mouse Renin1 Microplate (12 strips x 8 wells)", "number":"AB193728-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193728-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Renin Receptor also known as ATP6AP2 or the (pro)renin receptor is an important protein involved in the regulation of the renin-angiotensin system. It has a molecular mass of approximately 37 kDa. Expressed widely across several tissues including the kidneys heart and brain it acts by binding renin and prorenin leading to their activation and subsequent signaling. This receptor also facilitates cellular responses by increasing the efficiency of angiotensinogen conversion to angiotensin I which is a precursor to the potent vasoconstrictor angiotensin II.
Biological function summary

The Renin Receptor participates in maintaining homeostasis within the renin-angiotensin-aldosterone system. It potentially forms part of a larger receptor complex by interacting with other pathway components. By modulating the activity of renin and prorenin it enhances blood pressure regulation and fluid balance contributing to cardiovascular function and cellular ion homeostasis. Its involvement extends to promoting cell signaling events related to growth development and metabolism through the MAPK pathway.

Pathways

The Renin Receptor integrates into the renin-angiotensin system and the Wnt signaling pathway. In the renin-angiotensin system the receptor interacts with proteins such as angiotensin-converting enzyme (ACE) and angiotensin receptors playing an important role in blood pressure and electrolyte balance. Its engagement in the Wnt pathway suggests a role in development and cellular proliferation highlighting its interaction with proteins involved in cellular communication and embryonic development.

The Renin Receptor is linked to hypertension and cardiovascular diseases. Its overactivity or dysregulation may lead to elevated blood pressure due to increased angiotensin II formation. Additionally it is associated with heart failure where it contributes to pathological cardiac remodeling. The receptor's interaction with ACE and the renin-angiotensin system components highlights its involvement in these cardiovascular disorders suggesting potential targets for therapeutic intervention.
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Product protocols

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Target data

Multifunctional protein which functions as a renin, prorenin cellular receptor and is involved in the assembly of the lysosomal proton-transporting V-type ATPase (V-ATPase) and the acidification of the endo-lysosomal system (By similarity). May mediate renin-dependent cellular responses by activating ERK1 and ERK2 (By similarity). By increasing the catalytic efficiency of renin in AGT/angiotensinogen conversion to angiotensin I, may also play a role in the renin-angiotensin system (RAS) (By similarity). Through its function in V-type ATPase (v-ATPase) assembly and acidification of the lysosome it regulates protein degradation and may control different signaling pathways important for proper brain development, synapse morphology and synaptic transmission (PubMed : 26376863, PubMed : 29127204, PubMed : 30985297).
See full target information Atp6ap2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Bioscience reports 45:157-170 PubMed39869501

2025

Carbonyl reductase 1: a novel regulator of blood pressure in Down syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra J Malbon,Alicja Czopek,Andrew M Beekman,Zoë R Goddard,Aileen Boyle,Jessica R Ivy,Kevin Stewart,Scott G Denham,Joanna P Simpson,Natalie Z Homer,Brian R Walker,Neeraj Dhaun,Matthew A Bailey,Ruth A Morgan

Foods (Basel, Switzerland) 13: PubMed38790847

2024

Modulatory Effects of the Kuwanon-Rich Fraction from Mulberry Root Bark on the Renin-Angiotensin System.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Hae Lee,Heon-Woong Kim,So-Ah Kim,Wan-Taek Ju,Seong-Ryul Kim,Hyun-Bok Kim,Ik-Seob Cha,Seong-Wan Kim,Jong-Woo Park,Sang-Kuk Kang

Cell & bioscience 12:197 PubMed36471394

2022

Activation of Piezo1 downregulates renin in juxtaglomerular cells and contributes to blood pressure homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqiang Yang,Honghui Zeng,Le Wang,Siweier Luo,Yiming Zhou

Frontiers in physiology 10:1118 PubMed31555149

2019

Vitamin D Receptor Deficiency Does Not Affect Blood Pressure and Heart Function.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah M Grundmann,Alexandra Schutkowski,Barbara Schreier,Sindy Rabe,Bettina König,Michael Gekle,Gabriele I Stangl
View all publications
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