Mouse Resistin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Resistin with a sensitivity of 6.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Frontiers in endocrinology 14:12377962023Adiponectin and resistin modulate the progression of Alzheimer´s disease in a metabolic syndrome model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPedro Cisternas,Camila Gherardelli,Joel Gutierrez,Paulina Salazar,Carolina Mendez-Orellana,G William Wong,Nibaldo C InestrosaPubMed 37732123
- Biomedicines 11:2023New Synthesized Activating Transcription Factor 3 Inducer SW20.1 Suppresses Resistin-Induced Metabolic Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTu T Tran,Wei-Jan Huang,Heng Lin,Hsi-Hsien ChenPubMed 37371606
- British journal of pharmacology 178:3924-39422021NLRP3 at the crossroads between immune/inflammatory responses and enteric neuroplastic remodelling in a mouse model of diet-induced obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarolina Pellegrini,Matteo Fornai,Laura Benvenuti,Rocchina Colucci,Valentina Caputi,Pablo Palazon-Riquelme,Maria Cecilia Giron,Anna Nericcio,Francesca Garelli,Vanessa D'Antongiovanni,Cristina Segnani,Chiara Ippolito,Monica Nannipieri,Gloria Lopez-Castejon,Pablo Pelegrin,György Haskó,Nunzia Bernardini,Corrado Blandizzi,Luca AntonioliPubMed 34000757
- Communications biology 2:3892019Adipocyte browning and resistance to obesity in mice is induced by expression of ATF3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChing-Feng Cheng et. al.PubMed 31667363
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 6.5 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes.
Alternative names
Fizz3, Retn, Resistin, Adipose tissue-specific secretory factor, Adipose-specific cysteine-rich secreted protein A12-alpha, Cysteine-rich secreted protein FIZZ3, ADSF
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Mouse Resistin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Resistin with a sensitivity of 6.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 6.5 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 5.1 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 7.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 91 | Range 88 - 95 % |
Sample type Cell culture media | Average % = 100 | Range 98 - 103 % |
Sample type Citrate plasma | Average % = 109 | Range 106 - 115 % |
Sample type EDTA Plasma | Average % = 95 | Range 90 - 103 % |
Sample type Heparin Plasma | Average % = 104 | Range 100 - 111 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse Resistin ELISA Kit (ab205574) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Resistin protein in cell culture extracts, cell culture supernatant, plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Resistin with 6.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mouse Resistin (RETN), also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone), is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family. Mouse Resistin is a 114-amino acid (aa) peptide (with a 20 aa signal sequence and a 94 aa mature segment) containing 11 cysteines that allow the association of several Resistin monomers into macromolecular complexes. Mouse Resistin is 75% and 56% identical to rat and human Resistin, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Resistin also known as FIZZ3 or ADSF is a protein with a mass of approximately 12.5 kDa. It is mainly expressed in adipose tissue and secreted by adipocytes. In human beings it is also found in inflammatory zones like macrophages and the placenta. Resistin functions as a signaling molecule or cytokine which plays roles in inflammation and metabolism.
- Biological function summary
Resistin affects glucose homeostasis and has been shown to modulate insulin resistance. It does not function as part of a larger protein complex but instead acts through specific receptors on cell surfaces. Resistin influences several cellular processes including energy balance and the modulation of inflammatory responses.
- Pathways
Several processes contribute to the detailed function of resistin. It is involved in the insulin signaling pathway and the inflammatory pathway. Resistin interacts with proteins and receptors like TLR4 which have key roles in inflammatory response signaling and metabolic regulation. These interactions can disrupt insulin signaling and glucose uptake in many cell types.
- Associated diseases and disorders
Resistin has connections to type 2 diabetes and obesity. Its role in insulin resistance makes it a significant molecule in the pathophysiology of type 2 diabetes. Additionally resistin can influence obesity through its effect on energy balance and fat distribution. Resistin's relationship with proteins like TNF-alpha and IL-6 further emphasizes its role in linking metabolic syndrome with chronic inflammatory states.
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7 product images
Sandwich ELISA - Mouse Resistin ELISA Kit (ab205574)
Mouse Resistin standard curve comparison data.
Standard curve comparison between mouse Resistin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity with shorter protocol time.
Sandwich ELISA - Mouse Resistin ELISA Kit (ab205574)
Example of the mouse Resistin standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Resistin ELISA Kit (ab205574)
Example of the mouse Resistin standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Resistin ELISA Kit (ab205574)
Linearity of dilution of mouse Resistin in serum and cell culture media.
Native mouse Resistin was measured in 2.5% mouse serum diluted in a 2-fold dilution series in Sample Diluent NS. Recombinant mouse Resistin was spiked into 10% cell culture media and diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse Resistin were measured in duplicate and interpolated from the mouse Resistin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
Sandwich ELISA - Mouse Resistin ELISA Kit (ab205574)
Linearity of dilution of mouse Resistin in plasma samples.
Native mouse Resistin was measured in 2.5% mouse plasma citrate, mouse plasma EDTA, and mouse plasma heparin samples diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse Resistin were measured in duplicate and interpolated from the mouse Resistin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
Sandwich ELISA - Mouse Resistin ELISA Kit (ab205574)
Linearity of mouse Resistin in 3T3-L1 adipocyte cell extract.
Native mouse Resistin was measured in 10 μg/mL of 3T3-L1 adipocyte cell extract diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. The concentrations of mouse Resistin were measured in duplicate and interpolated from the mouse Resistin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
Sandwich ELISA - Mouse Resistin ELISA Kit (ab205574)
Assay specificity is demonstrated on 3T3-L1 cells.
3T3-L1 cells were cultured in the presence and absence 1 μM Dexamethasone, 0.5 mM IBMX, 1 μg/mL insulin for 10 days to generate adipocytes and undifferentiated cells respectively. At the end of the treatment, protein extraction was carried out according to section 11.4 of the booklet. Both 3T3-L1 adipocytes and undifferentiated cell extracts were measured in duplicate at 10 μg/mL using this kit. The Raw O.D. values for each sample are graphed, with the background O.D. shown as the dashed line. The 3T3-L1 adipocytes were measured at 179 pg of Resistin/μg of cell lysate, whereas the 3T3-L1 undifferentiated cells measured below the limit of detection for this assay.
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