Mouse Resistin ELISA Kit

• sELISA

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Sandwich ELISA - Mouse Resistin ELISA Kit (AB205574)

Linearity of dilution of mouse Resistin in serum and cell culture media.

Native mouse Resistin was measured in 2.5% mouse serum diluted in a 2-fold dilution series in Sample Diluent NS. Recombinant mouse Resistin was spiked into 10% cell culture media and diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse Resistin were measured in duplicate and interpolated from the mouse Resistin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

• sELISA

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Sandwich ELISA - Mouse Resistin ELISA Kit (AB205574)

Example of the mouse Resistin standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

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Sandwich ELISA - Mouse Resistin ELISA Kit (AB205574)

Assay specificity is demonstrated on 3T3-L1 cells.

3T3-L1 cells were cultured in the presence and absence 1 μM Dexamethasone, 0.5 mM IBMX, 1 μg/mL insulin for 10 days to generate adipocytes and undifferentiated cells respectively. At the end of the treatment, protein extraction was carried out according to section 11.4 of the booklet. Both 3T3-L1 adipocytes and undifferentiated cell extracts were measured in duplicate at 10 μg/mL using this kit. The Raw O.D. values for each sample are graphed, with the background O.D. shown as the dashed line. The 3T3-L1 adipocytes were measured at 179 pg of Resistin/μg of cell lysate, whereas the 3T3-L1 undifferentiated cells measured below the limit of detection for this assay.

• sELISA

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Sandwich ELISA - Mouse Resistin ELISA Kit (AB205574)

Linearity of mouse Resistin in 3T3-L1 adipocyte cell extract.

Native mouse Resistin was measured in 10 μg/mL of 3T3-L1 adipocyte cell extract diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. The concentrations of mouse Resistin were measured in duplicate and interpolated from the mouse Resistin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

• sELISA

Supplier Data

Sandwich ELISA - Mouse Resistin ELISA Kit (AB205574)

Example of the mouse Resistin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

Supplier Data

Sandwich ELISA - Mouse Resistin ELISA Kit (AB205574)

Representative image of Mouse Resistin ELISA Kit (ab205574)

Components shown from left to right :

- Sample diluent NS

- 10X Wash Buffer PT (ab206977)

- 10X Mouse Resistin Detector Antibody

- Mouse Resistin Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Mouse Resistin Capture Antibody (Lyophilised)

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

• sELISA

Supplier Data

Sandwich ELISA - Mouse Resistin ELISA Kit (AB205574)

Linearity of dilution of mouse Resistin in plasma samples.

Native mouse Resistin was measured in 2.5% mouse plasma citrate, mouse plasma EDTA, and mouse plasma heparin samples diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse Resistin were measured in duplicate and interpolated from the mouse Resistin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

• sELISA

Unknown

Sandwich ELISA - Mouse Resistin ELISA Kit (AB205574)

Mouse Resistin standard curve comparison data.

Standard curve comparison between mouse Resistin SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity with shorter protocol time.