AB202404

Mouse Retinol Binding Protein 4 ELISA Kit

Recombinant
SimpleStep
What is this?

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(5 Publications)

Mouse Retinol Binding Protein 4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Retinol Binding Protein 4 with a sensitivity of 1.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Retinol-binding protein 4, Plasma retinol-binding protein, PRBP, RBP, Rbp4

7 Images

ELISA - Mouse Retinol Binding Protein 4 ELISA Kit (AB202404)

ELISA

Supplier Data

ELISA - Mouse Retinol Binding Protein 4 ELISA Kit (AB202404)

sELISA

Supplier Data

Sandwich ELISA - Mouse Retinol Binding Protein 4 ELISA Kit (AB202404)

Titration of mouse liver extract and mouse brain extract within the working range of the assay.

Interpolated values of RBP4 are plotted for the indicated tissue extracts based on an extract load of 30 μg/mL. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

sELISA

Lab

Sandwich ELISA - Mouse Retinol Binding Protein 4 ELISA Kit (AB202404)

Mouse Retinol Binding Protein 4 standard curve comparison data.

Standard curve comparison between mouse RBP4 SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 25-fold increase in sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Mouse Retinol Binding Protein 4 ELISA Kit (AB202404)

Example of RBP4 standard curve prepared in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Retinol Binding Protein 4 ELISA Kit (AB202404)

Quantitation of RBP4 expression in different tissue culture supernatants.

Background-subtracted data values (mean +/- SD, n = 2) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Retinol Binding Protein 4 ELISA Kit (AB202404)

Titration of mouse serum and plasma within the working range of the assay.

Background-subtracted data values (mean +/- SD, n = 2) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Retinol Binding Protein 4 ELISA Kit (AB202404)

Example of RBP4 standard curve prepared in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 1.6 pg/mL

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Retinol Binding Protein 4 ELISA Kit ab202404 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Retinol Binding Protein 4 in cell culture extracts, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts, urine. This SimpleStep sensitivity is 1.6 pg/mL.

How the assay works

Mouse Retinol Binding Protein 4 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Retinol Binding Protein 4 ELISA Kit ab202404 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-RBP4 antibody [EPR18020-10] - BSA and Azide free (Capture) ab242744
- Anti-RBP4 antibody [EPR18020-115] - BSA and Azide free (Detector) ab242744

Retinol-Binding Protein 4 (RBP4) is a small, highly conserved member of the lipocalin superfamily involved in the transportation of retinol (Vitamin A) in blood. Synthesized in hepatocytes and adipocytes, RBP4 is also an adipokine involved in insulin resistance in the AG4KO mouse model. Elevated serum levels of RBP4 are seen in obesity and Type 2 diabetes.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "97 - 112 %", "average": "= 105" }, { "sample": "Serum", "range": "96 - 106 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "94 - 103 %", "average": "= 99" }, { "sample": "Urine", "range": "94 - 104 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "91 - 96 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "92 - 104 %", "average": "= 99" }, { "sample": "Tissue Homogenate", "range": "104 - 130 %", "average": "= 112" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab202404-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB202404-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB202404-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB202404-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB202404-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB202404-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB202404-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse RBP4 Lyophilized Recombinant Protein", "number":"AB202404-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BC", "number":"AB202404-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse RBP4 Capture Antibody", "number":"AB202404-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse RBP4 Detector Antibody", "number":"AB202404-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB202404-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB202404-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RBP4 or retinol-binding protein 4 is a carrier protein that is essential for the transport of retinol or vitamin A in the bloodstream. Also known by the names RBP or plasma retinol-binding protein this protein has a molecular weight of approximately 21 kDa. RBP4 is mainly expressed in the liver and secreted into the blood where it binds to retinol and protects it from oxidation. The structure of RBP4 allows it to fit into binding pockets facilitating the transport of retinol to peripheral tissues.

Biological function summary

RBP4 plays a role as a transporter of vitamin A from the liver to tissues where it is needed. It forms complexes with transthyretin in the blood which prevents kidney filtration and subsequent loss of retinol. This function ensures the proper delivery and availability of retinol essential for vision cellular differentiation and immune system function. The stability of the retinol-RBP4 complex is important to maintaining sufficient vitamin A levels in the body.

Pathways

RBP4 is significant in the vitamin A metabolism pathway and affects the retinoid signaling pathways. These pathways are critical for processes such as vision and skin health. RBP4 interacts with proteins like lecithin retinol acyltransferase (LRAT) in these pathways which are responsible for esterifying retinol into retinyl esters a storage form of vitamin A. These interactions ensure the efficient transport and storage of vitamin A and that it is accessible when needed by different tissues.

RBP4 is linked to the development of insulin resistance and type 2 diabetes. Elevated RBP4 levels correlate with these conditions and highlight its role in metabolic processes. Studies also show a connection between altered RBP4 levels and obesity with insulin resistance often occurring in conjunction with obesity. The protein is implicated in these metabolic disorders through its interactions with insulin-regulating proteins which influence glucose homeostasis.

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Product protocols

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Target data

Retinol-binding protein that mediates retinol transport in blood plasma. Delivers retinol from the liver stores to the peripheral tissues. Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane.

See full target information Retinol-binding protein 4

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 15:1357932 PubMed38681142

2024

Parental recovered acute kidney injury causes prenatal renal dysfunction and fetal growth restriction with sexually dimorphic implications for adult offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica F Hebert,Yoshio Funahashi,Jacqueline M Emathinger,Megan N Nickerson,Tahnee Groat,Nicole K Andeen,Susan B Gurley,Michael P Hutchens

Endocrinology 163: PubMed35552670

2022

Intestinal Enteroendocrine Cell Signaling: Retinol-binding Protein 2 and Retinoid Actions.

Applications

Unspecified application

Species

Unspecified reactive species

Rossana M Calderon,Christopher A Smith,Emily L Miedzybrodzka,Josie A Silvaroli,Marcin Golczak,Fiona M Gribble,Frank Reimann,William S Blaner

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:15788-15804 PubMed33105029

2020

Knockout of Cyp26a1 and Cyp26b1 during postnatal life causes reduced lifespan, dermatitis, splenomegaly, and systemic inflammation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica M Snyder,Guo Zhong,Cathryn Hogarth,Weize Huang,Traci Topping,Jeffrey LaFrance,Laura Palau,Lindsay C Czuba,Michael Griswold,Gabriel Ghiaur,Nina Isoherranen

Laboratory investigation; a journal of technical m 98:512-524 PubMed29330472

2018

Amyloid deposition in a mouse model humanized at the transthyretin and retinol-binding protein 4 loci.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangshun Li,Yanyi Lyu,Jingling Shen,Yanshuang Mu,Lixia Qiang,Li Liu,Kimi Araki,Bruno P Imbimbo,Ken-Ichi Yamamura,Shoude Jin,Zhenghua Li

Laboratory investigation; a journal of technical m 97:395-408 PubMed28134916

2017

Rescue of retinal morphology and function in a humanized mouse at the mouse retinol-binding protein locus.

Applications

Unspecified application

Species

Unspecified reactive species

Li Liu,Tomohiro Suzuki,Jingling Shen,Shigeharu Wakana,Kimi Araki,Ken-Ichi Yamamura,Lei Lei,Zhenghua Li

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Retinol Binding Protein 4 ELISA Kit

ELISA - Mouse Retinol Binding Protein 4 ELISA Kit (AB202404)

Sandwich ELISA - Mouse Retinol Binding Protein 4 ELISA Kit (AB202404)

Sandwich ELISA - Mouse Retinol Binding Protein 4 ELISA Kit (AB202404)

Sandwich ELISA - Mouse Retinol Binding Protein 4 ELISA Kit (AB202404)

Sandwich ELISA - Mouse Retinol Binding Protein 4 ELISA Kit (AB202404)

Sandwich ELISA - Mouse Retinol Binding Protein 4 ELISA Kit (AB202404)

Sandwich ELISA - Mouse Retinol Binding Protein 4 ELISA Kit (AB202404)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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