JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB195459

Mouse Retinol binding protein 4 ELISA Kit (RBP4)

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Retinol binding protein 4 ELISA Kit (RBP4) is a Sandwich (quantitative) ELISA for the measurement of Mouse Retinol binding protein 4 (RBP4) in Mouse in Biofluids samples.

View Alternative Names

Retinol-binding protein 4, Plasma retinol-binding protein, PRBP, RBP, Rbp4

1 Images
Sandwich ELISA - Mouse Retinol binding protein 4 ELISA Kit (RBP4) (AB195459)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Retinol binding protein 4 ELISA Kit (RBP4) (AB195459)

Representative standard curve using ab195459.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 0.4 ng/mL

Range

0.625 - 40 ng/mL

Assay time

4h

Assay Platform

Microplate

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's Retinol binding protein 4 (RBP4) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of RBP4 concentrations in mouse plasma, serum, urine, cell lysate, and tissue samples.

An RBP4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an RBP4 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of RBP4 captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab195459-1x96Tests", "assetComponentDetails": [ { "size":"1 x 120 µL", "name":"50X Biotinylated Mouse RBP4 Antibody", "number":"AB195459-CMP10", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB195459-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Mouse RBP4 Standard (Lyophilised)", "number":"AB195459-CMP05", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB195459-CMP02", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB195459-CMP08", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB195459-CMP07", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB195459-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Mouse RBP4 Microplate (12 x 8 well strips)", "number":"AB195459-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB195459-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RBP4 or retinol-binding protein 4 is a carrier protein that is essential for the transport of retinol or vitamin A in the bloodstream. Also known by the names RBP or plasma retinol-binding protein this protein has a molecular weight of approximately 21 kDa. RBP4 is mainly expressed in the liver and secreted into the blood where it binds to retinol and protects it from oxidation. The structure of RBP4 allows it to fit into binding pockets facilitating the transport of retinol to peripheral tissues.
Biological function summary

RBP4 plays a role as a transporter of vitamin A from the liver to tissues where it is needed. It forms complexes with transthyretin in the blood which prevents kidney filtration and subsequent loss of retinol. This function ensures the proper delivery and availability of retinol essential for vision cellular differentiation and immune system function. The stability of the retinol-RBP4 complex is important to maintaining sufficient vitamin A levels in the body.

Pathways

RBP4 is significant in the vitamin A metabolism pathway and affects the retinoid signaling pathways. These pathways are critical for processes such as vision and skin health. RBP4 interacts with proteins like lecithin retinol acyltransferase (LRAT) in these pathways which are responsible for esterifying retinol into retinyl esters a storage form of vitamin A. These interactions ensure the efficient transport and storage of vitamin A and that it is accessible when needed by different tissues.

RBP4 is linked to the development of insulin resistance and type 2 diabetes. Elevated RBP4 levels correlate with these conditions and highlight its role in metabolic processes. Studies also show a connection between altered RBP4 levels and obesity with insulin resistance often occurring in conjunction with obesity. The protein is implicated in these metabolic disorders through its interactions with insulin-regulating proteins which influence glucose homeostasis.
style
left-column

Product protocols

style
left-column

Target data

Retinol-binding protein that mediates retinol transport in blood plasma. Delivers retinol from the liver stores to the peripheral tissues. Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane.
See full target information Retinol-binding protein 4
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB207620

Mouse Albumin ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Albumin ELISA Kit (AB207620)

  • 5
  • 1
Assay Kits

AB199082

Mouse Leptin ELISA Kit

SimpleStep

ELISA - Mouse Leptin ELISA Kit (AB199082)

  • 0
  • 0
Assay Kits

AB222511

Mouse C Reactive Protein ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse C Reactive Protein ELISA Kit (AB222511)

  • 0
  • 0
Assay Kits

AB205574

Mouse Resistin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse Resistin ELISA Kit (AB205574)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com