Mouse Retinol Binding Protein ELISA Kit (RBP)
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Mouse Retinol Binding Protein ELISA Kit (RBP) is a Sandwich (quantitative) ELISA for the measurement of Mouse Retinol Binding Protein (RBP) in Mouse in Biofluids samples.
View Alternative Names
Retinol-binding protein 3, Interphotoreceptor retinoid-binding protein, Interstitial retinol-binding protein, IRBP, Rbp3
- sELISA
Supplier Data
Sandwich ELISA - Mouse Retinol Binding Protein ELISA Kit (RBP) (AB190535)
Representative standard curve using ab190535 Retinol Binding Protein (RBP) Mouse ELISA Kit.
Reactivity data
Product details
Abcam's Retinol Binding Protein (RBP) Mouse ELISA kit (ab190535) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Retinol Binding Protein (RBP) in mouse serum and plasma.
In this assay the Retinol Binding Protein (RBP) present in samples reacts with the anti-Retinol Binding Protein (RBP) antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-RBP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound RBP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of RBP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of RBP in the test sample. The quantity of RBP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Properties and storage information
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Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RBP interacts with the cell membrane receptors to deliver retinol to target cells where it's needed for various physiological processes. It functions either independently or as part of a complex with transthyretin (TTR) in the extracellular space. This interaction with TTR stabilizes RBP and its retinol cargo in circulation and prevents renal clearance. The presence of this complex is significant for maintaining vitamin A homeostasis in the body.
Pathways
RBP serves an important role in retinoid metabolism and transport pathways. In the retinoid pathway RBP ensures the bioavailability of retinol which is further metabolized into active metabolites like retinal and retinoic acid. These metabolites are important for vision and gene regulation. RBP works closely with proteins such as STRA6 (Stimulated by Retinoic Acid Gene 6) a membrane receptor that facilitates retinol uptake into cells further connecting RBP to cellular retinoic acid signaling pathways.
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