Mouse RGMA ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse RGMA with a sensitivity of 90 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.31 - 20 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 90 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.
Alternative names
Repulsive guidance molecule A, RGM domain family member A, Rgma
What's included?
Recommended products
Mouse RGMA ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse RGMA with a sensitivity of 90 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.31 - 20 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 90 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 7 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 8.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 110 | Range 105 - 115 % |
Sample type EDTA Plasma | Average % = 112 | Range 105 - 121 % |
Sample type Heparin Plasma | Average % = 108 | Range 105 - 114 % |
Sample type Citrate plasma | Average % = 115 | Range 113 - 120 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse RGMA ELISA Kit (ab243687) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RGMA protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse RGMA with 90 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Repulsive Guidance Molecule A (RGMa) also known as RGM domain family member A is a glycoprotein involved in axon guidance and neuronal differentiation. It has a molecular weight of approximately 37 kDa. RGMa is expressed broadly in the central nervous system particularly in developing nervous tissues. Beyond the CNS RGMa is also found in various non-neuronal tissues. Its role in axonal repulsion is facilitated by its interaction with the Neogenin receptor influencing the directionality and pathfinding of growing nerve fibers.
- Biological function summary
RGMa plays a role in maintaining the structural organization of the nervous system by preventing aberrant nerve growth. RGMa forms part of a larger signaling complex that influences cell differentiation and migration. It acts by binding to Neogenin and recruiting co-receptors such as bone morphogenetic proteins (BMP) which regulate the differentiation and proliferation of neural progenitor cells. This interaction highlights its involvement in maintaining proper neural architecture and functionality.
- Pathways
RGMa engages in the BMP signaling pathway and the axon guidance pathway. Its interaction with BMP proteins affects the Smad signaling cascade ultimately influencing gene expression essential for neuronal differentiation. Furthermore RGMa's influence on axon guidance involves coordination with other guidance cues that help establish neural circuitry during development. Its functional relationship with Neogenin and BMP signaling pathways highlights its regulatory role in neural patterning.
- Associated diseases and disorders
RGMa is linked to conditions such as spinal cord injury and multiple sclerosis. In the context of spinal cord injury increased expression of RGMa contributes to the inhibition of neural regeneration due to its repulsive signaling effects. It interacts with other proteins including Neogenin to exacerbate the inhibitory environment post-injury. In multiple sclerosis RGMa regulates immune cell infiltration into the CNS linking it to disease progression and pathology. Understanding RGMa's role opens potential therapeutic avenues to modulate its activity in these disorders.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Sandwich ELISA - Mouse RGMA ELISA Kit (ab243687)
Mouse RGMA Standard Curve Comparison.
Standard Curve comparison between mouse RGMA SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Mouse RGMA ELISA Kit (ab243687)
Example of mouse RGMA standard curve in Sample Diluent 50BP.
The RGMA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse RGMA ELISA Kit (ab243687)
Interpolated concentrations of native RGMA in mouse serum and plasmas.
The concentrations of RGMA were measured in duplicates, interpolated from the RGMA standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (EDTA) 25%, plasma (citrate) 25% and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RGMA concentration was determined to be 24.1 ng/mL in serum, 15.5 ng/mL in plasma (EDTA), 17.3 ng/mL in plasma (citrate) and 23.8 ng/mL plasma (heparin).
Sandwich ELISA - Mouse RGMA ELISA Kit (ab243687)
Interpolated concentrations of native RGMA in rat serum and plasmas.
The concentrations of RGMA were measured in duplicates, interpolated from the RGMA standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (EDTA) 50%, plasma (citrate) 25% and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RGMA concentration was determined to be 5.3 ng/mL in serum, 4.2 ng/mL in plasma (EDTA), 4.1 ng/mL in plasma (citrate) and 5.3 ng/mL plasma (heparin).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com