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AB285283

Mouse S100B ELISA Kit

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(1 Publication)

Mouse S100B ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse S100B in Mouse in Biofluids samples.

View Alternative Names

Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100b

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Sandwich ELISA - Mouse S100B ELISA Kit (AB285283)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse S100B ELISA Kit (AB285283)

Mouse S100B ELISA Kit.

Typical standard curve and data is provided below for reference only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Other biological fluids

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 46.88 pg/mL

Range

78.13 - 5000 pg/mL

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This Mouse S100B ELISA kit (ab285283, E4754) is based on Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to mouse S100B. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for mouse S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of S100B bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of mouse S100B. The concentration of mouse S100B in the samples can be calculated by comparing the OD of the samples to the standard curve.

This product is manufactured by BioVision, an Abcam company and was previously called E4754 S100B (Mouse) ELISA Kit. E4754-100 is the same size as the 96 test size of ab285283.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.37" } ]
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Recovery

[ { "sample": "Serum", "range": "89 - 105 %", "average": "= 96" }, { "sample": "Plasma", "range": "91 - 106 %", "average": "= 99" } ]
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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285283-96Test", "assetComponentDetails": [ { "size":"1 x 120 µL", "name":"100X HRP-Conjugate", "number":"AB285283-CMP01", "productcode":"" }, { "size":"1 x 120 µL", "name":"100X Biotinylated Detection Antibody", "number":"AB285283-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Standard", "number":"AB285283-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample and Standard Diluent", "number":"AB285283-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB285283-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB285283-CMP08", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Plate Sealer", "number":"AB285283-CMP09", "productcode":"" }, { "size":"1 x 14 mL", "name":"Biotinylated Detection Antibody Diluent", "number":"AB285283-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"Substrate Reagent", "number":"AB285283-CMP10", "productcode":"" }, { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB285283-CMP11", "productcode":"" }, { "size":"1 x 14 mL", "name":"HRP-Conjugate Diluent", "number":"AB285283-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100 beta also known as protein S100 is part of the S100 family of proteins characterized by their calcium-binding properties. The S100 beta protein has an approximate molecular mass of 10.7 kDa. This protein is mainly expressed in astrocytes a type of glial cell in the central nervous system. It acts as a marker for astrocytes and is prominently used in astrocyte immunohistochemistry. Additionally it serves as a valuable marker for identifying and studying astrocyte markers because it can highlight the presence of these cells in various contexts.
Biological function summary

The S100 beta protein participates in several intracellular and extracellular activities influencing cell proliferation and differentiation. It forms dimers and sometimes assembles into larger complexes mediated by the binding of calcium ions which alters its functional state. Within the nervous system S100 beta modulates the dynamics between neurons and glial cells affecting processes such as cell survival and neurite outgrowth. This dual role in both supporting and inhibiting various cellular actions highlights its importance in maintaining neural homeostasis.

Pathways

S100 beta engages in signaling pathways related to inflammation and stress responses. It works within the MAPK and NF-kB pathways playing roles in cellular responses to oxidative stress. These pathways involve interactions with other proteins like p53 which S100 beta can modulate to influence apoptosis. The regulatory properties of S100 beta in these pathways link it with broader networks affecting cell fate decisions under pathological and physiological conditions.

S100 beta is associated with neurodegenerative diseases and cancer. Elevated levels of S100 beta are often found in Alzheimer's disease where it interacts with proteins like amyloid-beta contributing to neuroinflammation and neurotoxicity. In cancer particularly malignant melanoma S100 beta levels serve as a biomarker for tumor progression. Its interactions with p53 and other regulatory proteins highlight its involvement in oncogenic processes emphasizing its potential as a target for therapeutic intervention in these diseases.
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Product protocols

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Target data

Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain (By similarity). Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer (By similarity). Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites (By similarity). Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation (PubMed : 2592414, PubMed : 8202493). Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue (PubMed : 31043739). Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase (By similarity). Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization (By similarity). May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (By similarity).
See full target information S100b
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 11: PubMed35624723

2022

Antioxidant Polyphenols of Medicinal Plant and Caffeic Acid Reduce Cerebrovascular, Inflammatory and Metabolic Disorders Aggravated by High-Fat Diet-Induced Obesity in a Mouse Model of Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Janice Taïlé,Matthieu Bringart,Cynthia Planesse,Jessica Patché,Philippe Rondeau,Bryan Veeren,Patricia Clerc,Anne Gauvin-Bialecki,Steeve Bourane,Olivier Meilhac,David Couret,Marie-Paule Gonthier
View all publications
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websiteProtocolBooklet
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